Abstract
Human islet amyloid polypeptide (hIAPP or amylin) is a polypeptide hormone produced in the pancreatic β-cells that plays a role in glycemic control. hIAPP is deficient in type 1 and type 2 diabetes and is a promising adjunct to insulin therapy. However, hIAPP rapidly forms amyloid, and its strong tendency to aggregate limits its usefulness. The process of hIAPP amyloid formation is toxic to cultured β-cells and islets, and islet amyloid formation in vivo has been linked to β-cell death and islet graft failure. An analogue of hIAPP with a weakened tendency to aggregate, denoted pramlintide (PM), has been approved for clinical applications, but suffers from poor solubility, particularly at physiological pH, and its unfavorable solubility profile prevents coformulation with insulin. We describe a strategy for rationally designing analogues of hIAPP with improved properties; key proline mutations are combined with substitutions that increase the net charge of the molecule. An H18R/G24P/I26P triple mutant and an H18R/A25P/S28P/S29P quadruple mutant are significantly more soluble at neutral pH than hIAPP or PM. They are nonamyloidogenic and are not toxic to rat INS β-cells. The approach is not limited to these examples; additional analogues can be designed using this strategy. To illustrate this point, we show that an S20R/G24P/I26P triple mutant and an H18R/I26P double mutant are nonamyloidogenic and significantly more soluble than human IAPP or PM. These analogues and second-generation derivatives are potential candidates for the coformulation of IAPP with insulin and other polypeptides.
Insulin therapy is the most widely used treatment for diabetes. Despite improvements in insulin therapy over the past few decades, the goal of achieving complete glycemic control in diabetic patients has still not been achieved. Postprandial hyperglycemia persists in diabetes, even with aggressive insulin therapy, due, in part, to the imbalance of secreted hormones that normally work together to achieve normal glucose homeostasis.1
Human islet amyloid polypeptide (hIAPP or amylin) is a neuropancreatic hormone produced in the pancreatic β-cells. The polypeptide is stored in the insulin secretory granule and cosecreted with insulin.2,3 Mature hIAPP is 37 residues in length and contains an amidated C-terminus and a disulfide bond between Cys-2 and Cys-7 (Figure 1). hIAPP normally complements insulin by suppressing postprandial glucagon secretion, helping to regulate the rate of gastric emptying, and inducing satiety.4−7 The polypeptide forms amyloid in the pancreatic islets of Langerhans in type 2 diabetes by an unknown mechanism. hIAPP amyloid deposits are associated with reduced β-cell mass, and islet amyloidosis is believed to contribute to type 2 diabetes.2,8−11 Islet amyloidosis also contributes to the failure of islet transplantation, and recent work has shown that prevention of islet amyloid formation enhances graft survival.12,13 hIAPP also aggregates aggressively in vitro and is one of the most aggregation prone naturally occurring sequences known.
hIAPP is deficient in both type 1 and type 2 diabetes patients14−16 and is a promising adjunct to insulin therapy. However, clinical use of hIAPP is not practical because of its aggressive tendency to aggregate in vitro, leading to difficulties in formulation and storage, and because some hIAPP aggregates are toxic to β-cells.
Not all species form islet amyloid, and the ability to do so correlates with the primary sequence of IAPP. A number of nonamyloidgenic IAPP variants contain multiple proline substitutions, a well-known breaker of β-structure within the segment of residues 20–29.17,18 Early work led to the suggestion that the sequence in this region controls the amyloidogenicity of the polypeptide. The situation has subsequently been shown to be more complex. Variations outside of the segment of residues 20–29 can influence significantly the amyloidogenicity of IAPP, and multiple proline substitutions in other segments of the molecule can inhibit amyloid formation.19,20 In addition, the charge state of residue 18 and the charge state of the N-terminus play an important role in modifying the tendency to aggregate and form amyloid.21 Nevertheless, substitutions within the region of residues 20–29 are very important in determining the amyloidogencity of IAPP.20,22−24 Analysis of the relationship between sequence and amyloidogenicity has led to the development of IAPP analogues, based upon rat IAPP (rIAPP), that are less prone to aggregation than hIAPP.
rIAPP differs from the human polypeptide at six positions, with five of the substitutions, including three prolines, found between residues 20 and 29. rIAPP is not toxic, does not form amyloid, and is more soluble than hIAPP, due in part to the three prolines at positions 25, 28, and 29.18,25 These residues are Ala, Ser, and Ser, respectively, in the human polypeptide (Figure 1). The sequence differences between rat and human IAPP have been exploited to develop a more soluble analogue of hIAPP, pramlintide (PM, also known as Symlin), that has been clinically approved as an adjunct to insulin therapy, in which residues 25, 28, and 29 of hIAPP are substituted with proline (Figure 1). These substitutions render the human peptide nonamyloidgenic under typical conditions. Clinical studies show that PM retains the activity of hIAPP, and the addition of PM to insulin therapy improves postprandial glycemic control in patients with type 1 and type 2 diabetes.1,26−30
PM still has solubility issues, particularly at physiological pH. PM is more soluble at acidic pH where the N-terminus and His-18 will be fully protonated and is formulated under these conditions, while insulin is formulated at near neutral pH. This prevents the coformulation of insulin and PM, leading to increased cost in combination therapies and potentially reducing patient compliance, due to the need for multiple injections. There is also interest in the coformulation of IAPP or PM with other proteins, and IAPP analogues with improved solubility should be useful in this context, as well.31−33
Here, we describe an approach for rationally designing analogues of hIAPP with improved solubility that involves minimal substitutions. The strategy relies on mutating select residues to proline and making an additional substitution to increase the net charge of the molecule. We demonstrate the approach with an H18R/G24P/I26P triple mutant (TM-a) and an H18R/Ala-25/Ser-28/Ser-29 quadruple mutant (QM). Both are significantly more soluble than hIAPP or PM, and both are nonamyloidogenic and are not toxic to INS-1 β-cells. The approach is not limited to these two cases, and additional analogues can be designed using this strategy (Figure 1). We illustrate this point with an S20R/G24P/I26P triple mutant (TM-b) and an H18R/I26P double mutant (DM).
Materials and Methods
Peptide Synthesis
All peptides were synthesized on a 0.1 mmol scale using a CEM microwave peptide synthesizer. 9-Fluorenylmethoxycarbonyl (Fmoc) chemistry was utilized. 5-(4′-Fmoc-aminomethyl-3′,5-dimethoxyphenol) valeric acid (PAL-PEG) resin was used to afford an amidated C-terminus. For hIAPP, TM-a, TM-b, and DM, Fmoc-protected pseudoproline (oxazolidine) dipeptide derivatives were incorporated to improve the yield as previously described.34 For PM and QM, only Fmoc-Ala-Thr(ΨMe, Mepro)-OH and Fmoc-Leu-Ser(ΨMe, Mepro)-OH were utilized. Standard Fmoc reaction cycles were used as previously described.35 The first residue attached to the resin, all β-branched residues, and all pseudoproline dipeptide derivatives were double-coupled. The peptides were cleaved from the resin using standard trifluoroacetic acid (TFA) methods.
Oxidation and Purification of Peptides
Crude peptides collected after cleavage were dissolved in 20% (v/v) acetic acid and lyophilized. This step was repeated several times before oxidation and purification to improve the solubility of the peptides. The peptides were oxidized in 100% dimethyl sulfoxide at room temperature to form the disulfide bond and then purified via reverse-phase high-performance liquid chromatography (RP-HPLC) using a Vydac C18 preparative column.36 The masses of the pure peptides was confirmed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry: hIAPP, expected 3903.6, observed 3904.6; PM, expected 3949.3, observed 3949.2; QM, expected 3969.4, observed 3968.1; TM-a, expected 3946.9, observed 3945.7; TM-b, expected 3997.4, observed, 3997.1; DM, expected 3907.3, observed, 3908.1.
Sample Preparation
Each peptide was dissolved in 100% hexafluoroisoproponal (HFIP) to make a 1.6 mM stock solution. Stock solutions were filtered using a 0.45 μM Acrodisc syringe filter with a GHP membrane, and the required amount of peptide was lyophilized overnight to remove HFIP. Dry peptide was dissolved into the appropriate buffer for the fluorescence assays.
Fluorescence Assays
Thioflavin-T binding assays, conducted without stirring at 25 °C, were utilized to monitor amyloid formation kinetics. Fluorescence measurements were performed using a Beckman Coulter DTX 880 plate reader with a multimode detector using an excitation wavelength of 430 nm and an emission wavelength of 485 nm. Samples were prepared by dissolving the dry peptide into Tris-HCl buffer and a thioflavin-T solution immediately before the measurement. The final concentrations were 16 μM hIAPP or 160 μM analogue (each one) and 32 μM thioflavin-T in 20 mM Tris-HCl (pH 7.4).
Solubility Measurements
Dry peptides were dissolved in PBS buffer containing 10 mM PO43–, 137 mM NaCl, and 2.7 mM KCl (pH 7.4) at different initial concentrations and incubated for 7 days at 25 °C without being stirred. Each sample was then centrifuged using a Beckman Coulter Microfuge 22R centrifuge at 24 °C for 20 min. The relative centrifugal force used was 1.75 × 104g. The solubility of each sample was approximated by measuring the absorbance of the corresponding supernatant at 280 nm measured using a Beckman Coulter DU 730 UV/vis spectrophotometer. All of the peptides contain a single Tyr, three Phe residues, and a disulfide bond and no Trp; thus, their extinction coefficients at 280 nm are identical.
Liquid Chromatography–Mass Spectroscopy (LC–MS) Experiments
Samples were prepared by dissolving insulin, at an equimolar ratio, with either PM, DM, or TM-a in PBS buffer (pH 7.4) at an initial concentration of 500 μM. Aliquots were removed at time zero (control). Additional aliquots were removed after incubation for 24 h and centrifuged (17500g for 20 min). Aliquots were immediately frozen with liquid nitrogen and lyophilized. LC–UV–MS measurements were taken by redissolving the samples and immediately injecting them. Samples were analyzed using an Agilent 1260 HPLC instrument and an Agilent G6224A TOF mass spectrometer. The HPLC method used a Kinetex C18 column [100 Å, 2.6 μm, 100 mm × 2.1 mm (Phenomenex)] at 35 °C and a flow rate of 0.60 mL/min. The HPLC solvents were (A) H2O [0.1% (v/v) acetic acid and 0.1% (v/v) TFA] and (B) CH3CN [0.1% (v/v) acetic acid and 0.1% (v/v) TFA]. The HPLC solvent gradient consisted of the following: 10% B from 0 to 1.5 min, 10 to 50% B from 1.5 to 21.5 min, and 50 to 95% B from 21.5 to 31.5. Mass spectra were acquired in the range of m/z 300–3200 with internal calibration using five standards in profile mode at 5 Hz. Electrospray ionization in the positive mode was used: cap voltage of 4000 V, gas temperature of 325 °C, drying gas of 12 L/min, nebulizer of 55 psi, and fragmentor voltage of 200 V.
UV chromatograms were acquired with a diode array detector (DAD) operating with a 10 Hz acquisition rate and a 2 nm bandwidth in the range of 205–500 nm. The absorbance was recorded at 215 ± 8 and 280 ± 8 nm with a 500 ± 50 nm reference wavelength to obtain integrated UV areas. ESI+, TIC (total ion current) mass chromatograms were integrated, and averaged mass spectra were acquired from the integrated peaks with background subtraction. The mass spectra of the target peptides were observed in predominantly the +3 and +4 charge states. The resolution of the mass spectrometer in the described state and in this m/z range is ∼13000. This resolution allows the isotopic distribution of the peptide m/z peaks in these charge states to be fully resolved. Mass spectra were deconvoluted using the Agilent Resolved Isotope Deconvolution algorithm. The monoisotopic, neutral masses were determined with an accuracy of ±10 ppm. The extracted ion chromatograms (EIC) were used with the two most intense m/z ions from the +3 and +4 charge states for relative quantitation from the mass chromatograms.
Transmission Electron Microscopy (TEM)
TEM images were collected at the Life Science Microscopy Center at the State University of New York at Stony Brook. Aliquots (15 μL) of the samples used for fluorescence assays were removed at the end of each kinetic experiment, blotted on a carbon-coated 200-mesh copper grid for 1 min, and then negatively stained with saturated uranyl acetate for 1 min.
Circular Dichroism (CD)
Far-UV CD experiments were performed on an Applied Photophysics Chirascan CD spectrophotometer at 25 °C. Aliquots from the kinetic experiments were removed at the end of each experiment, and the spectra were recorded as the average of three repeats over a range of 190–260 nm, at 1 nm intervals. A 0.1 cm quartz cuvette was used, and a background spectrum was subtracted from the data.
Cell Culture
Transformed rat insulinoma-1 (INS-1) pancreatic β-cells were grown in RPMI 1640 supplemented with 10% fetal bovine serum (FBS), 11 mM glucose, 10 mM Hepes, 2 mM l-glutamine, 1 mM sodium pyruvate, 50 μM β-mercaptoethanol, 100 units/mL penicillin, and 100 units/mL streptomycin. Cells were maintained at 37 °C under 5% CO2.
AlamarBlue Cell Viability Assays
Cytotoxicity was measured by AlamarBlue reduction assays. INS-1 β-cells were seeded at a density of 30000 cells/well in 96-well plates and cultured for 24 h prior to being stimulated with wild-type hIAPP and mutant IAPP peptides. Peptides dissolved in RPMI were added directly to cells and incubated on cells for 24 and 48 h. AlamarBlue was diluted 10-fold in culture media and incubated on cells for an additional 5 h at 37 °C. The fluorescence (excitation at 530 nm and emission at 590 nm) was measured on a Beckman Coulter DTX880 fluorescent plate reader. Values were calculated relative to those of control cells treated with buffer only. All values represent means ± the standard error of the mean (n = 4).
Results and Discussion
Design of Soluble Analogues
The rate of amyloid formation by hIAPP is strongly dependent on pH and is significantly faster above neutral pH, because of changes in the ionization state of the N-terminus and His-18.21,37−39 PM and hIAPP both contain a His residue at position 18, while rIAPP contains an Arg. These observations led us to suspect that the solubility of PM could be significantly increased by ensuring that residue 18 remains positively charged in the pH range of interest. The two choices for substitution among the genetically encoded amino acids are Lys and Arg. Either would accomplish this goal; however, the Arg replacement is found in a number of IAPP sequences, while the Lys replacement is not. In addition, the amino group in Lys is generally more reactive than the guanidino group of Arg because of its lower pKa; thus, we chose to replace His-18 in the human peptide with Arg to generate the quadruple mutant, H18R/A25P/S28P/S29P IAPP, denoted QM. This mutant can also be viewed as an H18R mutant of pramlintide.
It is natural to inquire if three proline substitutions are required and if the proline substitutions need to be located at positions 25, 28, and 29; thus, we designed a second analogue that contains only two prolines in addition to the H18R substitution. A single proline mutation at position 24 or 26 of hIAPP weakens the tendency of the peptide to aggregate and converts the peptide into a moderately effective inhibitor of amyloid formation by wild-type hIAPP.22,40 The motivation for originally choosing these sites was that substitutions with proline were shown to have large effects on amyloid formation by a set of 10-residue fragments of hIAPP.41 However, each of the single-proline mutants of hIAPP still forms nonamyloid aggregates at neutral pH, even at low concentrations; thus, we prepared an analogue (TM-a) in which both residues 24 and 26 were mutated to proline and which included the H18R mutation.
The Analogues Do Not Form Amyloid in a Homogenous Solution
We first tested the propensity of the different analogues to form amyloid at pH 7.4 using thioflavin-T fluorescence assays and transmission electron microscopy (TEM). Thioflavin-T is a small dye that undergoes an increase in quantum yield upon binding to amyloid fibrils and provides a convenient probe for monitoring the kinetics of amyloid formation. The dye does not perturb the kinetics of hIAPP amyloid formation under the conditions used here. Amyloid formation follows a sigmoidal time course consisting of a lag phase, in which few or no fibrils are formed, followed by a growth phase and then a saturation phase in which amyloid fibrils are in equilibrium with the soluble peptide. Amyloid formation by hIAPP reached the saturation phase within 40 h under the conditions used here, while none of the analogues tested (PM, TM-a, and QM) formed any detectable amyloid during the time course of the experiments (∼140 h) as indicated by flat fluorescence curves and by TEM, even though they were examined at a concentration 10-fold higher than that of hIAPP (Figure 2). The thioflavin-T fluorescence signal derives from the fibril-bound dye, and the intensity is highly dependent on how well the dye binds; hence, thioflavin-T studies can sometimes be misleading.42 Therefore, we conducted TEM measurements of the samples collected at the end of each kinetic experiment. TEM images of hIAPP showed typical amyloid fibril morphology, while no fibrils were found in the TEM images of samples of the three analogues (PM, TM-a, and QM) (Figure 3).
The Analogues Are Significantly More Soluble Than PM at Neutral pH
We next compared the apparent solubilities of PM, TM-a, and QM at pH 7.4. Each peptide was incubated in PBS buffer containing 10 mM PO43–, 137 mM NaCl, and 2.7 mM KCl for 7 days at three different initial concentrations (100 μM, 500 μM, and 1 mM), and the solution was then centrifuged. The apparent solubility of each sample was approximated by the absorbance of the supernatant measured at 280 nm. The extinction coefficients of all of the polypeptides are identical at 280 nm because they contain the same aromatic residues and each contains a disulfide bond; thus, the absorbance of the supernatant is a direct readout of the relative solubility of the different samples. A sample of hIAPP, prepared at an initial concentration of 1 mM, was used as a control. At 100 μM, there were detectable differences in the amount of peptide in the soluble fraction. The supernatant of the QM sample had an absorbance, 0.16, higher than that of TM-a (0.10) or PM (0.11) (Figure 4). At higher concentrations (500 μM and 1 mM), the amount of peptide remaining in solution was significantly higher for both analogues than for PM. At 1 mM, the absorbances of the soluble fractions of TM-a and QM were 1.2 and 1.3, respectively, while the value for the PM sample was only 0.35. In comparison, the absorbance of the supernatant of the 1 mM hIAPP sample was just 0.11 (Figure 4). For the samples prepared at an initial concentration of 500 μM, the absorbances of the supernatants of the TM-a and QM solutions were 0.61 and 0.72, respectively, and both were significantly higher than that of PM (0.33) (Figure 4).
Neither TM-a nor QM Is Toxic to β-Cells
We tested the effects of the analogues on cell viability using rat INS-1 β-cells, a pancreatic cell line that is commonly employed in studies of hIAPP toxicity. Cell viability was monitored by AlamarBlue assays for peptide samples at 21 and 42 μM. hIAPP was used as a positive control. Incubating INS-1 β-cells with 21 μM hIAPP led to clearly detectable toxicity; cell viability was reduced to 62 ± 4% relative to that of the medium alone control after incubation for 24 h and to 54 ± 5% after incubation for 48 h. In contrast, incubation of cells with either TM-a or QM at 21 μM barely decreased cell viability (Figure 5). Increasing the hIAPP concentration to 42 μM resulted in even more significant effects; cell viability was reduced to only 14 ± 9% after incubation for 24 h and 9 ± 9% after incubation for 48 h. In striking contrast, no obvious cytotoxicity was observed for TM-a or QM at 42 μM (Figure 5). These results demonstrate that, unlike hIAPP, TM-a and QM are not toxic to cells at the concentrations examined.
The Strategy Can Be Extended To Include Charged Substitutions at Different Sites
We wanted to test if a charge substitution at a different site would also lead to an enhanced solubility and reduced amyloidogenicity. We chose to target Ser-20. Substitution of a glycine at this site leads to accelerated amyloid formation, while replacement with a Lys has been shown to slow but not prevent amyloid formation.43
We prepared a S20R/G24P/I26P triple mutant (TM-b) and tested both its ability to form amyloid and its apparent solubility at neutral pH. Thioflavin-T fluorescence experiments and TEM confirmed that TM-b did not form amyloid fibrils during the time course of the experiments, even at a peptide concentration of 160 μM (Figure 6). We then compared the apparent solubility of TM-b with PM and TM-a at pH 7.4 using the same method described above (Figure 7). TM-b was more soluble than PM and TM-a. The absorbances of the soluble fractions of samples of TM-b, TM-a, and PM prepared at an initial concentration of 100 μM were 0.16, 0.10, and 0.11, respectively. Examination of samples prepared at higher initial concentrations revealed that TM-b was much more soluble than PM and somewhat more soluble than TM-a. We tested samples prepared at initial concentrations of 500 μM and 1 mM. The absorbances of the supernatants of samples of TM-b prepared at initial concentrations of 500 μM and 1 mM were 0.79 and 1.5, respectively, while the values for PM were 0.33 and 0.35, respectively.
Multiple Proline Substitutions Are Not Required To Design Soluble Analogues
Single-proline substitutions have been reported to reduce the amyloidgenicity of hIAPP.22 To test if multiple proline substitutions together with the charged mutations are required to improve the solubility at neutral pH, we tested an H18R/I26P double mutant of hIAPP (DM). This peptide did not form amyloid during the time course of the experiments, as demonstrated by a flat thioflavin-T fluorescence curve and by TEM (Figure 8). DM was more soluble at neutral pH than PM, even though this peptide has only one proline substitution, while PM has three (Figure 9). The absorbances of the soluble fractions of 1 mM, 500 μM, and 100 μM samples of DM measured after incubation for 1 week were 1.2 for the 1 mM sample, 0.67 for the 500 μM sample, and 0.10 for the 100 μM sample.
Designed Analogues Are More Soluble Than Pramlintide in the Presence of Insulin
We examined a subset of the analogues in the presence of insulin to test if they are more soluble than PM when insulin is present. We chose TM-a and DM for comparison to PM, because TM-a and DM differ from each other only at position 24, which is a proline in TM-a. Comparison of the two analogues, thus, tests if multiple Pro substitutions are required for increased solubility in the presence of insulin. The three polypeptides were individually incubated with equimolar insulin at an initial concentration of 500 μM in PBS buffer containing 10 mM PO43–, 137 mM NaCl, and 2.7 mM KCl at pH 7.4, and the amount of each IAPP analogue remaining in solution after 24 h was analyzed. Samples were centrifuged and aliquots of the supernatant analyzed by LC–MS. Insulin could be separated from the analogues by LC (Supporting Information), allowing quantification of the amount of each IAPP analogue remaining in solution. The amount of PM in the soluble fraction was notably reduced after 24 h, with more than 37% of the material having been lost, as judged by the absorbance at 280 nm, or by the extracted ion chromatography (EIC) peak intensity (Supporting Information). The two designed analogues were more soluble than PM in the presence of insulin, and considerably less material was lost to the insoluble fraction (<15% for TM-a and <17% for DM). Any difference between the two designed analogues was modest, as judged by the absorbance of the samples, indicating that a single, strategically placed, proline together with a charge substitution is capable of generating an analogue that has improved solubility in the presence of insulin.
Conclusions
We have developed a simple strategy for designing nonamyloidgenic, nontoxic analogues of hIAPP with solubility at neutral pH significantly better than that of PM. The strategy includes a minimum combination of mutations that increases the net charge of the peptide with single or multiple proline substitutions. Our results demonstrate that the approach is not limited to a specific site and there is no strict requirement for the number of proline substitutions. In this case, we have localized the proline substitutions within the region of residues 20–29; however, it is known that multiple proline substitutions outside this segment can reduce amyloidogenicity, and thus, the approach may be even more general.19 It is likely that N-methylated amino acids could also be used, because they, like proline, are β-sheet breakers.44,45 Nongenetically encoded amino acids such as ornithine could likely be used instead of Lys or Arg. Of course using just the genetically encoded amino acids facilities peptide production by recombinant means. Analogues such as the ones described here, and next-generation variants, are potential adjuncts to insulin therapy that will allow coformulation with insulin.
Acknowledgments
We thank Dr. Ping Cao for assistance with the toxicity assays. We thank Mr. Tyler Wilkinson for assistance with peptide synthesis and purification.
Glossary
Abbreviations
- CD
circular dichroism
- DM
H18R/I26P double mutant
- EIC
extracted ion chromatograms
- FBS
fetal bovine serum
- Fmoc
9-fluorenylmethoxycarbonyl
- HFIP
hexafluoroisoproponal
- hIAPP
human islet amyloid polypeptide
- INS-1
rat insulinoma-1
- LC–MS
liquid chromatography–mass spectrometry
- PAL-PEG
5-(4′-Fmoc-aminomethyl-3′,5-dimethoxyphenol) valeric acid
- PM
pramlintide
- QM
H18R/Ala-25/Ser-28/Ser-29 quadruple mutant
- rIAPP
rat IAPP
- RP-HPLC
reverse-phase high-performance liquid chromatography
- TEM
transmission electron microscopy
- TM-a
H18R/G24P/I26P triple mutant
- TM-b
S20R/G24P/I26P triple mutant.
Supporting Information Available
LC chromatographs from LC–MS measurements of mixtures of insulin with PM, TM-a, and DM recorded for freshly dissolved (control, time zero) samples and for samples that were incubated for 24 h with insulin; plots showing the normalized integrated intensity of the extracted ion chromatography peak intensity of PM, TM-a, and DM for freshly dissolved (control, time zero) samples and samples that were incubated for 24 h with insulin; and plots showing the normalized integrated absorbance at 280 nm of PM, TM-a, and DM for freshly dissolved (control, time zero) samples and samples that were incubated for 24 with insulin. This material is available free of charge via the Internet at http://pubs.acs.org.
This work was supported by National Institutes of Health Grant GM078114 to D.P.R.
The authors declare a competing financial interest. A patent for the design and application of soluble IAPP analogues has been submitted.
Funding Statement
National Institutes of Health, United States
Supplementary Material
References
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