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. 2014 Sep 10;53(37):5831–5833. doi: 10.1021/bi500966b

Figure 1.

Figure 1

Bovine retina HITS-CLIP library production and analysis. (a) Autoradiograms of labeled protein–RNA complexes generated by CLIP using either normal IgG or the Argonaute-specific 2A8 mouse monoclonal antibody. (b and c) PCR amplification of cDNA derived from Argonaute CLIP on bovine retina. (d and e) Bovine retina Argonaute HITS-CLIP and transcriptome sequencing results for the miRNA target genes Ppp2ca and Rgs9bp were mapped onto the UCSC genome browser. High-stringency enrichment analysis requiring high-confidence peaks in all three biological replicates identified a single high-confidence miRNA target site in each transcript.