FIG 7 .

Nix glycoside hydrolases of X. campestris pv. campestris are partially epistatic to the nagA phenotype during plant infection and growth in cabbage xylem sap. (A and B) X. campestris pv. campestris strains (OD₆₀₀ = 0.05) were inoculated by piercing leaves of Arabidopsis thaliana ecotype Sf-2. (A) Disease index scored at 8 days postinoculation. Results are given as percentages of the value for the wild-type (wt) strain. Standard deviations were obtained from at least 16 inoculated leaves from four plants. (B) IGC for the inoculated area. Results are given as CFU per cm² of leaves. Standard deviations were obtained from at least 3 independent samples. (C) Growth of X. campestris pv. campestris strains in xylem sap from Brassica oleracea cv. Bartolo F1 cabbage (OD₆₀₀ = 0.01 at time zero). (D) Growth of X. campestris pv. campestris strains in MME minimal medium containing chitotetraose (0.1% [wt/vol]). Standard deviations were obtained from at least three independent experiments. (E) Growth of X. campestris pv. campestris strains in MMC minimal medium (OD₆₀₀ = 0.1 at time zero). (C and E) At different time points, CFU were counted by plating serial dilutions. Standard deviations were obtained from at least three independent experiments. Values significantly different (P < 0.0001) from that for the wt strain (a), both the wt and ΔnagA ampG*I strains (b), and the ΔnagA strain (c) are indicated.