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. 1995 May 23;92(11):5154–5158. doi: 10.1073/pnas.92.11.5154

Human TAFII31 protein is a transcriptional coactivator of the p53 protein.

H Lu 1, A J Levine 1
PMCID: PMC41867  PMID: 7761466

Abstract

The p53 protein activates transcription of a target gene by binding to a specific DNA response element and interacting with the transcriptional apparatus of RNA polymerase II. The amino-terminal domain of p53 interacts with a component of the TFIID basal transcription complex. The human TATA-binding-protein-associated factor TAFII31, a component of TFIID, has been identified as a critical protein required for p53-mediated transcriptional activation. TAFII31 and p53 proteins bind to each other via amino acid residues in the amino-terminal domain of p53 that are essential for transcription. Antibodies directed against TAFII31 protein inhibit p53-activated but not basal transcription in vitro. These results demonstrate that TAFII31 is a coactivator for the p53 protein.

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Selected References

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