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| Method | Hanging-drop vapour diffusion |
| Plate type | 24-well plates from Hampton Research |
| Temperature (C) | 20 |
| Protein concentration (mgml1) | 68 |
| Buffer composition of protein solution | 20mM TrisHCl pH 8.0, 500mM NaCl |
| Composition of reservoir solution | 0.4M formate dihydrate, 0.2M NDSB-221, 0.1M bis-tris pH 5.0 |
| Volume and ratio of drop | 2l, 1:1 |
| Volume of reservoir (l) | 500 |
