Table 2. Summary of Targeted Proteomic Analyses of ETI Protein Complexes.
| Target | Tag | System | Purification Strategya | Proteins Identified | Reference | |
|---|---|---|---|---|---|---|
| HopN1 (T3SE) | 6xHIS | N. benthamiana | In vitro pulldown, Ni2+-IMAC | PsbQ | 72 | |
| RPS2 (NB-LRR-protein) | HA-PreScission-Biotin (HBP) | A. thaliana | Biotin/Streptavidin AP | RIN4 (At3g25070) AtHIR1 (At1g69840) AtHIR2 (At3g01290) |
BSK1 (At4g35230) BSK8 (At5g41260) |
73 |
| Prf (NB-LRR-protein) | SBP-FLAG | Tomato | TAP, Streptavidin AP/FLAG IAP | Pto Fen |
Pth2 Pth3 |
80 |
| Pto (Monitored by Prf) | FLAG | Tomato | FLAG IAP | Prf Fen |
Pth3 Pth 5 |
|
| RIN4 (Monitored by RPM1/RPS2) | A. thaliana | Native IAP | RIPK (AT2G05940) | 50 | ||
| MOS4 (mediates NB-LRR protein signaling) | HA | A. thaliana | HA IAP | AtCDC5 (At1g09770) PRL1 (At4g15900) |
MAC3A (At1g04510) MAC3B (At2G33340) |
86 |
Note: aAP: affinity purification, IAP: immunoaffinity purification