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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 2014 Sep 12;111(39):14307. doi: 10.1073/pnas.1417135111

Correction for Kuscu et al., CRL4-like Clr4 complex in Schizosaccharomyces pombe depends on an exposed surface of Dos1 for heterochromatin silencing

PMCID: PMC4191748

BIOCHEMISTRY Correction for “CRL4-like Clr4 complex in Schizosaccharomyces pombe depends on an exposed surface of Dos1 for heterochromatin silencing,” by Canan Kuscu, Mikel Zaratiegui, Hyun Soo Kim, David A. Wah, Robert A. Martienssen, Thomas Schalch, and Leemor Joshua-Tor, which appeared in issue 5, February 4, 2014, of Proc Natl Acad Sci USA (111:1795–1800; first published January 21, 2014; 10.1073/pnas.1313096111).

The authors note that Fig. 2 and its corresponding legend appeared incorrectly. The corrected figure and its corrected legend appear below. In addition, the authors note that on page 1797, right column, last paragraph, Fig. 2C should appear as Fig. 2B.

Fig. 2.

Fig. 2.

The WD40 repeat domain of Dos1 is essential but not sufficient for heterochromatin formation at the S. pombe centromere. (A) Schematic diagram of S. pombe centromere 1. The position of the centromeric otr1R::ura4 reporter insertion used in this study is indicated. Comparative growth assay of the serially diluted dos1 null strain with the centromeric otr1R::ura4 reporter expressing the indicated Dos1 fragments from a plasmid. Strains were examined for growth on pombe glutamate media (PMG) lacking leucine and supplemented with 1 g/L 5-FOA (+FOA –Leu), PMG media lacking uracil and leucine (−Ura −Leu), and PMG media lacking leucine (–Leu). Cells were always grown on a PMG medium lacking leucine to select for Dos1 expressing plasmid. (B) OSS-Rik1AC was coexpressed with FLAG-Dos1 truncations and pulled down with Strep-Tactin beads to detect whether the interactions are still preserved in Dos1 truncations.


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