MICROBIOLOGY Correction for “Kaposi’s sarcoma-associated herpesvirus LANA recruits the DNA polymerase clamp loader to mediate efficient replication and virus persistence,” by Qiming Sun, Toshiki Tsurimoto, Franceline Juillard, Lin Li, Shijun Li, Erika De León Vázquez, She Chen, and Kenneth Kaye, which appeared in issue 32, August 12, 2014, of Proc Natl Acad Sci USA (111:11816–11821; first published July 28, 2014; 10.1073/pnas.1404219111).
The authors note that Fig. 3 appeared incorrectly. The corrected figure and its legend appear below.
Fig. 3.
LANA interaction with RFC is critical for LANA-mediated episome persistence. (A) BJAB or BJAB/LANA outgrowth in microtiter plates after seeding at 1,000, 100, or 10 cells per well in the presence or absence of RFC1 knockdown (KD). Averages of three experiments are shown. Error bars indicate SD. (B) G418-resistant outgrowth of BJAB or BJAB/LANA cells after p8TR transfection with or without RFC1 knockdown. Averages of three experiments, with SD, are shown. (C) Gardella gel analysis (27) assessing the presence of episomal DNA in BJAB or BJAB/LANA cells with or without RFC1 KD after 20 d of G418 selection. Numbers refer to independently derived G418-resistant cell lines expanded from individual microtiter wells. The two leftmost lanes have increasing amounts of naked p8TR plasmid. O, gel origin. (D) Western blot analysis for LANA, RFC1, or Tub in cell lines used for Gardella gel analysis (27) in C. The asterisk indicates nonspecific bands. (E) LANA immunostaining in the indicated cell lines from C with or without RFC1 KD. Cell lines 1, 5, and 6 (WT, Upper) or cell lines 9, 1, and 3 (RFC1 KD, Lower) contain successively lower levels of episomal DNA as observed in C. Broad nuclear LANA staining indicates episome loss (arrowheads), whereas LANA dots (circled cells) indicate sites of episomes. (Magnification: 630×.) (F) Quantification of average percentage of cells containing LANA dots. Averages of three experiments, with SD, are shown.