Abstract
BRCA1 is a breast/ovarian cancer susceptibility gene on human chromosome 17q21. We describe a complete and detailed physical map of a 500-kb region of genomic DNA containing the BRCA1 gene and the partial cloning in phage P1 artificial chromosomes. Approximately 70 exons were isolated from this region, 11 of which were components of the BRCA1 gene. Analysis of the other exons revealed a rho-related G protein and the interferon-induced leucine-zipper protein IFP-35.
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- Anderson L. A., Friedman L., Osborne-Lawrence S., Lynch E., Weissenbach J., Bowcock A., King M. C. High-density genetic map of the BRCA1 region of chromosome 17q12-q21. Genomics. 1993 Sep;17(3):618–623. doi: 10.1006/geno.1993.1381. [DOI] [PubMed] [Google Scholar]
- Bange F. C., Vogel U., Flohr T., Kiekenbeck M., Denecke B., Böttger E. C. IFP 35 is an interferon-induced leucine zipper protein that undergoes interferon-regulated cellular redistribution. J Biol Chem. 1994 Jan 14;269(2):1091–1098. [PubMed] [Google Scholar]
- Brown M. A., Nicolai H., Xu C. F., Griffiths B. L., Jones K. A., Solomon E., Hosking L., Trowsdale J., Black D. M., McFarlane R. Regulation of BRCA1. Nature. 1994 Dec 22;372(6508):733–733. doi: 10.1038/372733a0. [DOI] [PubMed] [Google Scholar]
- Buckler A. J., Chang D. D., Graw S. L., Brook J. D., Haber D. A., Sharp P. A., Housman D. E. Exon amplification: a strategy to isolate mammalian genes based on RNA splicing. Proc Natl Acad Sci U S A. 1991 May 1;88(9):4005–4009. doi: 10.1073/pnas.88.9.4005. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Campbell I. G., Nicolai H. M., Foulkes W. D., Senger G., Stamp G. W., Allan G., Boyer C., Jones K., Bast R. C., Jr, Solomon E. A novel gene encoding a B-box protein within the BRCA1 region at 17q21.1. Hum Mol Genet. 1994 Apr;3(4):589–594. doi: 10.1093/hmg/3.4.589. [DOI] [PubMed] [Google Scholar]
- Easton D. F., Bishop D. T., Ford D., Crockford G. P. Genetic linkage analysis in familial breast and ovarian cancer: results from 214 families. The Breast Cancer Linkage Consortium. Am J Hum Genet. 1993 Apr;52(4):678–701. [PMC free article] [PubMed] [Google Scholar]
- Hall J. M., Lee M. K., Newman B., Morrow J. E., Anderson L. A., Huey B., King M. C. Linkage of early-onset familial breast cancer to chromosome 17q21. Science. 1990 Dec 21;250(4988):1684–1689. doi: 10.1126/science.2270482. [DOI] [PubMed] [Google Scholar]
- Ioannou P. A., Amemiya C. T., Garnes J., Kroisel P. M., Shizuya H., Chen C., Batzer M. A., de Jong P. J. A new bacteriophage P1-derived vector for the propagation of large human DNA fragments. Nat Genet. 1994 Jan;6(1):84–89. doi: 10.1038/ng0194-84. [DOI] [PubMed] [Google Scholar]
- Jones K. A., Black D. M., Brown M. A., Griffiths B. L., Nicolai H. M., Chambers J. A., Bonjardim M., Xu C. F., Boyd M., McFarlane R. The detailed characterisation of a 400 kb cosmid walk in the BRCA1 region: identification and localisation of 10 genes including a dual-specificity phosphatase. Hum Mol Genet. 1994 Nov;3(11):1927–1934. [PubMed] [Google Scholar]
- Miki Y., Swensen J., Shattuck-Eidens D., Futreal P. A., Harshman K., Tavtigian S., Liu Q., Cochran C., Bennett L. M., Ding W. A strong candidate for the breast and ovarian cancer susceptibility gene BRCA1. Science. 1994 Oct 7;266(5182):66–71. doi: 10.1126/science.7545954. [DOI] [PubMed] [Google Scholar]
- Nobes C., Hall A. Regulation and function of the Rho subfamily of small GTPases. Curr Opin Genet Dev. 1994 Feb;4(1):77–81. doi: 10.1016/0959-437x(94)90094-9. [DOI] [PubMed] [Google Scholar]
- Peltoketo H., Isomaa V., Vihko R. Genomic organization and DNA sequences of human 17 beta-hydroxysteroid dehydrogenase genes and flanking regions. Localization of multiple Alu sequences and putative cis-acting elements. Eur J Biochem. 1992 Oct 1;209(1):459–466. doi: 10.1111/j.1432-1033.1992.tb17310.x. [DOI] [PubMed] [Google Scholar]