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. 2014 Jul 23;5(16):6716–6733. doi: 10.18632/oncotarget.2236

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Copyright: © 2014 Wang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

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(A) Changes in mRNA expression of the indicated genes in two EIF5A2-suppressed HCC cell lines were assessed by real-time qPCR. (B) Analysis of MMP-2 activity in TCM by gelatin zymography. TCM was collected from tumor cells transfected with NC or shRNA-EIF5A2 or with control vector or eIF5A2 over-expression vector. Recombinant human MMP2 (rHuMMP2) as the positive control. (C) Analysis of MMP-2 expression in xenograft tumor tissue sections. (D) Inhibition of MMP-2 (final concentration of anti-MMP2 antibody: 6ug/ml) repressed the pro-angiogenic effect of EIF5A2 over-expression in SMMC7721 cells. (E) Re-expression of MMP-2 in EIF5A2 knockdown PLC8024 cells restored the pro-angiogenic effect of EIF5A2.