Fig.7. 78Fc as an NIR optical imaging tracer in the human TEM1-expressing tumor vascular model.

Human (A) and mouse TEM1 mRNA (B) expression in control and hTEM1 expressing tumor grafts. TEM1 mRNA levels were evaluated with qRT-PCR. TEM1 expression in normal human or murine cervix was normalized to 1. 10⁷ MS1-huTEM1 or control MS1 cells were injected subcutaneously into nude mice on the left or right flanks, respectively (n=3), and tumor grafts were harvested after the indicated time (2-3 weeks). huTEM1-positive tumor grafts express muTEM1 at similar levels to that of control tumor grafts. C, in vivo bioluminescence imaging (BLI) and 78Fc750- or IgG-750-based live NIR optical imaging in huTEM1-positive and control tumor grafts (n=3 per group). Green circle, MS1-TEM1 tumor; yellow circle, MS1 control tumor. D (Also in Sup videos), 78Fc750 BLI and NIR tomographic imaging in mice grafted with MS1-huTEM1 (lower) and MS1 (upper) in nude mice. Bioluminescent signals overlap with infrared signals only in the huTEM1-positive tumors. From left, picture (area inside the purple square is used for 3D dual modality imaging to regenerate mice surface), 3D reconstructions of bioluminescence (DLIT) and NIR fluorescence (FLIT); superimposed DLIT with FLIT. n=5.