Figure 8.

SNXA does not affect NIMX^CDC2 histone ¹H kinase activity. To determine if snxA1 suppresses nimX2 by affecting NIMX protein levels or activity, snxA+/nimX+ (lanes 1 and 5), nimX2 (lanes 2 and 6), snxA1 (lanes 3 and 7) and snxA1/nimX2 (lanes 4,8) were grown at the nimX2 permissive temperature (32°) or germinated at 32° and shifted to restrictive temperature (42°) for 2 hr. Histone ¹H kinase assays were performed on anti-NIMX immunoprecipitates followed by SDS-PAGE. A total of 100 ug total protein was separated by SDS-PAGE, blotted to nitrocellulose, and detected with anti-NIMX antibody E77.