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. 2014 Oct;141(20):4018–4030. doi: 10.1242/dev.115709

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© 2014. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 6. — The PU.1-14kb element is active at sites of mouse definitive haematopoiesis in vivo. (A) Schematic of the PU.1 locus highlighting the relevant cis-regulatory elements. (B) Reporter constructs used for transient transgenic embryo generation. (C) Representative lacZ⁺ whole-mount images and section images (original magnification: 40×) of the dorsal aorta of E11.5 transgenic embryos carrying the reporters illustrated above in B. Insets (original magnification: 100×) show cell clusters budding from the ventral side of the dorsal aorta. The number of lacZ⁺ embryos/number of total PCR⁺ embryos analysed is indicated in each whole-mount image.