Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Oct;141(20):4018–4030. doi: 10.1242/dev.115709

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

PMC Copyright notice

Fig. 7. — PU.1 expression is induced during the EHT in vivo. (A) Fli1, PU.1 and Myb single-cell RT-qPCR gene expression data from Swiers et al. (2013) in the AGM region and vitelline and umbilical artery (AGM+VUA) for VEcad⁺ cell populations from E10.5 embryos carrying a Runx1+23kb-GFP enhancer reporter. Gene expression levels are displayed as a heatmap of ΔCt relative to housekeeping genes (Ubc and Atp5a1). (B) Genotype schematics and flow cytometry plots displaying VEcad versus PU.1-YFP expression for the Ter119⁻ (Ly76⁻) cell population from the AGM+VUA of E10.5 WT (left) and homozygous PU.1-YFP transgenic (right) embryos. Data were collected from pooled embryos and are representative of two E10-10.5 embryo litters. (C) Flow cytometry plots displaying CD41 versus CD45 expression for the populations within the VEcad/PU.1-YFP gates in B for the homozygous PU.1-YFP transgenic embryos.