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. 1995 May 9;92(10):4596–4600. doi: 10.1073/pnas.92.10.4596

Intrinsic transcript cleavage activity of RNA polymerase.

M Orlova 1, J Newlands 1, A Das 1, A Goldfarb 1, S Borukhov 1
PMCID: PMC41991  PMID: 7538676

Abstract

The GreA and GreB transcript cleavage factors of Escherichia coli suppress elongation arrest and may have a proofreading role in transcription. With the use of E. coli greA-greB- mutant, RNA polymerase is demonstrated to possess substantial intrinsic transcript cleavage activity. Mildly alkaline pH mimics the effect of the Gre proteins by inducing transcript cleavage in ternary complexes and antagonizing elongation arrest through a cleavage-and-restart reaction. Thus, transcript cleavage constitutes the second enzymological activity of RNA polymerase along with polymerization/pyrophosphorolysis of RNA, whereas the Gre proteins merely enhance this intrinsic property.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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