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. 2014 Nov;20(11):1815–1826. doi: 10.1261/rna.044321.114

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© 2014 Tian et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article, published in RNA, is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 5. — Equilibrium fitting tests a register-shift “excited state” model for the 126–235 RNA. (A) Linear fitting of P4a/shift-P4a equilibrium based on G204. An enlarged view of G204 reactivities is on the right. Averaged reactivity profiles of P4a-stabilized (blue) and shift-P4a-stablized (green) double mutants are plotted. SDs are shown, N = 5. (B) Model of 126–235 RNA secondary structure in solution, including P4a/shift-P4a dynamics.