Figure 5. Effect of SKi on the phosphorylation status of signaling pathways controlling survival, proliferation.

and stress response. (A) Molt-4, Jurkat and CEM-R cells were treated with SKi for 40 h. Then, cells were collected, lysed, and analyzed by western blotting. Protein (50 μg) was loaded in each lane.β-tubulin was used as a loading control. (B-C) Primary cells from T-ALL patients were treated with SKi (10 μM) for 24 h, stained with various antibodies or Annexin-V/PI and subjected to cytometric analysis.