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. 2014 May 13;10(7):1285–1300. doi: 10.4161/auto.28789

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Copyright © 2014 Landes Bioscience

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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graphic file with name auto-10-1285-g9.jpg — Figure 9. The MAPK11/12/13/14 (p38 MAPK) inhibitor SB203580 decreases HYF127c/Cu-induced autophagy. (A) The function of 652 genes (fold > 1 or fold < −1, P < 0.01) analyzed by DAVID. (B) IPA analysis of phosphorylation pathways possibly involved in HYF127c/Cu-induced autophagy and cell death. (C) Phosphorylation of MAPK11/12/13/14 (p38 MAPK) and its downstream target protein MAPKAPK2 in HYF127c/Cu-treated HeLa cells. (D) Effect of the combination of SB203580 and HYF127c/Cu on cellular viability in HeLa cells, (n = 3, *P < 0.05). (E) Decreased punctate distribution of EGFP-LC3 in cells treated with HYF127c/Cu and SB203580 (i). Bar, 20 μM. The percentage of cells with evident accumulation of EGFP-LC3 dots (ii) and the average number of EGFP-LC3 dots in cells (iii) (n = 3, *P < 0.05). (F) Western blot results of SQSTM1 and LC3 in cells treated with both HYF127c/Cu and SB203580 or treated with HYF127c/Cu alone. (G) The effect of SB203580 on cellular viability of HYF127c/Cu-treated Atg7^+/+ and atg7^−/− cells (n = 3, *P < 0.05).