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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Glia. 2014 Jul 17;62(12):2034–2043. doi: 10.1002/glia.22724

Figure 1.

Figure 1

Effects of DPI on cell viability and DA uptake capacity in primary midbrain neuron-glia cultures incubated with different concentrations of DPI for 48 h. (A) Cell viability was evaluated by MTT assays. (B) Representative images of cells immunostained with Neu-N, Iba-1 and GFAP antibodies indicate lesions on the neurons, microglia and astroglia after micromolar, but not subpicomolar, DPI exposure. The inserts show amplified microglia in each group. (C) [3H]-DA uptake analysis revealed a decrease in neurotransmitter uptake capacity after micromolar, but not subpicomolar, DPI exposure. The results are expressed as a percentage of the controls (mean ± SEM) from three experiments performed in duplicate and were analyzed using one-way ANOVA, followed by Bonferroni’s post hoc multiple comparison test. **p < 0 .01; Bar = 50 μm.