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. 1976 Jan;13(1):16–21. doi: 10.1128/iai.13.1.16-21.1976

Effect of glucose and sucrose on survival in batch culture of Streptococcus mutans C67-1 and a noncariogenic mutant, C67-25.

H D Donoghue, H N Newman
PMCID: PMC420570  PMID: 2556

Abstract

The growth and survival of two strains of Streptococcus mutans in 5% (wt/vol) glucose or sucrose broth was investigated. S. mutans strain C67-1 showed little loss of viability after 30 h of incubation in batch culture in the presence of either sugar. S. mutans strain C67-25, a noncariogenic mutant of C67-1 that has lost the ability of the latter to produce sticky, insoluble extracellular polysaccharide when grown in sucrose broth, showed a dramatic loss of viability after 30 h of incubation in either glucose or sucrose broth, the effect being most marked in the presence of glucose. The loss of viability was shown to be due to acid production. Insoluble extracellular polysaccharide production appears to be a phenomenon favoring the survival of organisms subjected to high sucrose levels. Other factors must be involved, however, since there are differences between the two strains as regards their survival in glucose broth.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bowen W. H. The effects of calcium, magnesium and manganese on dextran production by a cariogenic streptococcus. Arch Oral Biol. 1971 Jan;16(1):115–119. doi: 10.1016/0003-9969(71)90142-7. [DOI] [PubMed] [Google Scholar]
  2. Bowen W. H. The trace element requirements of cariogenic and non-cariogenic streptococci. Arch Oral Biol. 1968 Jun;13(6):713–714. doi: 10.1016/0003-9969(68)90151-9. [DOI] [PubMed] [Google Scholar]
  3. Carlsson J. A levansucrase from Streptococcus mutans. Caries Res. 1970;4(2):97–113. doi: 10.1159/000259632. [DOI] [PubMed] [Google Scholar]
  4. Coykendall A. L., Specht P. A., Samol H. H. Streptococcus mutans in a wild, sucrose-eating rat population. Infect Immun. 1974 Jul;10(1):216–219. doi: 10.1128/iai.10.1.216-219.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Critchley P., Wood J. M., Saxton C. A., Leach S. A. The polymerisation of dietary sugars by dental plaque. Caries Res. 1967;1(2):112–129. doi: 10.1159/000259506. [DOI] [PubMed] [Google Scholar]
  6. De Stoppelaar J. D., Van Houte J., Backer Dirks O. The relationship between extracellular polysaccharide-producing streptococci and smooth surface caries in 13-year-old children. Caries Res. 1969;3(2):190–199. doi: 10.1159/000259582. [DOI] [PubMed] [Google Scholar]
  7. Donoghue H. D., Tyler J. E. Antagonisms amongst streptococci isolated from the human oral cavity. Arch Oral Biol. 1975 May-Jun;20(5-6):381–387. doi: 10.1016/0003-9969(75)90031-x. [DOI] [PubMed] [Google Scholar]
  8. Dugan P. R., MacMillan C. B., Pfister R. M. Aerobic heterotrophic bacteria indigenous to pH 2.8 acid mine water: predominant slime-producing bacteria in acid streamers. J Bacteriol. 1970 Mar;101(3):982–988. doi: 10.1128/jb.101.3.982-988.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Dunny G. M., Birch N., Hascall G., Clewell D. B. Isolation and characterization of plasmid deoxyribonucleic acid from Streptococcus mutans. J Bacteriol. 1973 Jun;114(3):1362–1364. doi: 10.1128/jb.114.3.1362-1364.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gibbons R. J., Banghart S. B. Synthesis of extracellular dextran by cariogenic bacteria and its presence in human dental plaque. Arch Oral Biol. 1967 Jan;12(1):11–23. doi: 10.1016/0003-9969(67)90137-9. [DOI] [PubMed] [Google Scholar]
  11. Gibbons R. J., Berman K. S., Knoettner P., Kapsimalis B. Dental caries and alveolar bone loss in gnotobiotic rats infected with capsule forming streptococci of human origin. Arch Oral Biol. 1966 Jun;11(6):549–560. doi: 10.1016/0003-9969(66)90220-2. [DOI] [PubMed] [Google Scholar]
  12. Gibbons R. J., Fitzgerald R. J. Dextran-induced agglutination of Streptococcus mutans, and its potential role in the formation of microbial dental plaques. J Bacteriol. 1969 May;98(2):341–346. doi: 10.1128/jb.98.2.341-346.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Gibbons R. J. Formation and significance of bacterial polysaccharides in caries etiology. Caries Res. 1968;2(2):164–171. doi: 10.1159/000259554. [DOI] [PubMed] [Google Scholar]
  14. Greer S. B., Hsiang W., Musil G., Zinner D. D. Viruses of cariogenic streptococci. J Dent Res. 1971 Nov-Dec;50(6):1594–1604. doi: 10.1177/00220345710500064101. [DOI] [PubMed] [Google Scholar]
  15. HAMMOND B. F., WILLIAMS N. B. STUDIES ON ENCAPSULATED LACTOBACILLI. I. UTILIZATION OF CAPSULAR MATERIAL BY LACTOBACILLUS CASEI. Arch Oral Biol. 1964 May-Jun;9:341–349. doi: 10.1016/0003-9969(64)90066-4. [DOI] [PubMed] [Google Scholar]
  16. Hammond B. F. Intracellular polysaccharide production by human oral strains of Lactobacillus casei. Arch Oral Biol. 1971 Mar;16(3):323–338. doi: 10.1016/0003-9969(71)90024-0. [DOI] [PubMed] [Google Scholar]
  17. Harris R. H., Mitchell R. The role of polymers in microbial aggregation. Annu Rev Microbiol. 1973;27:27–50. doi: 10.1146/annurev.mi.27.100173.000331. [DOI] [PubMed] [Google Scholar]
  18. Higuchi M., Endo K., Hoshino E., Araya S. Preferential induction of rough variants in Streptococcus mutans by ethidium bromide. J Dent Res. 1973 Sep-Oct;52(5):1070–1075. doi: 10.1177/00220345730520051401. [DOI] [PubMed] [Google Scholar]
  19. Kelstrup J., Funder-Nielsen T. D. Molecular interactions between the extracellular polysaccharides of Streptococcus mutans. Arch Oral Biol. 1972 Dec;17(12):1659–1670. doi: 10.1016/0003-9969(72)90228-2. [DOI] [PubMed] [Google Scholar]
  20. Klein J. P., Frank R. M. Mise en évidence de virus dans les bactéries cariogénes de la plaque dentaire. J Biol Buccale. 1973 Mar;1(1):79–85. [PubMed] [Google Scholar]
  21. Miller C. H. Degradation of sucrose by whole cells and plaque of Actinomyces naeslundii. Infect Immun. 1974 Dec;10(6):1280–1291. doi: 10.1128/iai.10.6.1280-1291.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Newman H. N. Microbial films in nature. Microbios. 1974 Mar-Apr;9(36):247–257. [PubMed] [Google Scholar]
  23. Newman H. N., Poole D. F. Structural and ecological aspects of dental plaque. Soc Appl Bacteriol Symp Ser. 1974;3(0):111–134. [PubMed] [Google Scholar]
  24. Parsons A. B., Dugan P. R. Production of extracellular polysaccharide matrix by Zoogloea ramigera. Appl Microbiol. 1971 Apr;21(4):657–661. doi: 10.1128/am.21.4.657-661.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. ROREM E. S. Uptake of rubidium and phosphate ions by polysaccharide-producing bacteria. J Bacteriol. 1955 Dec;70(6):691–701. doi: 10.1128/jb.70.6.691-701.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Rogers A. H. Bacteriocin production and susceptibility among strains of Streptococcus mutans grown in the presence of sucrose. Antimicrob Agents Chemother. 1974 Nov;6(5):547–550. doi: 10.1128/aac.6.5.547. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Schachtele C. F., Loken A. E., Schmitt M. K. Use of specifically labeled sucrose for comparison of extracellular glucan and fructan metabolism by oral streptococci. Infect Immun. 1972 Feb;5(2):263–266. doi: 10.1128/iai.5.2.263-266.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Tanzer J. M., Freedman M. L., Fitzgerald R. J., Larson R. H. Diminished virulence of glucan synthesis-defective mutants of Streptococcus mutans. Infect Immun. 1974 Jul;10(1):197–203. doi: 10.1128/iai.10.1.197-203.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Tanzer J. M., Wood W. I., Krichevsky M. I. Linear growth kinetics of plaque-forming streptococci in the presence of sucrose. J Gen Microbiol. 1969 Sep;58(1):125–133. doi: 10.1099/00221287-58-1-125. [DOI] [PubMed] [Google Scholar]
  30. WILKINSON J. F. The extracellualr polysaccharides of bacteria. Bacteriol Rev. 1958 Mar;22(1):46–73. doi: 10.1128/br.22.1.46-73.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. de Stoppelaar J. D., König K. G., Plasschaert A. J., van der Hoeven J. S. Decreased cariogenicity of a mutant of Streptococcus mutans. Arch Oral Biol. 1971 Aug;16(8):971–975. doi: 10.1016/0003-9969(71)90186-5. [DOI] [PubMed] [Google Scholar]
  32. van Houte J., Winkler K. C., Jansen H. M. Iodophilic polysaccharide synthesis, acid production and growth in oral streptococci. Arch Oral Biol. 1969 Jan;14(1):45–61. doi: 10.1016/0003-9969(69)90020-x. [DOI] [PubMed] [Google Scholar]
  33. van der Hoeven J. S. A slime-producing microorganism in dental plaque of rats, selected by glucose feeding. Chemical composition of extracellular slime elaborated by Actinomyces viscosus, strain Nyl. Caries Res. 1974;8(3):193–210. doi: 10.1159/000260109. [DOI] [PubMed] [Google Scholar]

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