Abstract
Intravenous injection of the thermostable direct hemolysin (lethal toxin) produced by Vibrio parahaemolyticus caused rapid death of rats. Studies by electroencephalography and electrocardiography showed that after intravenous injection of the toxin the electroencephalogram remained normal for quite a long time after the heart of the animals had stopped beating. Depression of intraatrial and intraventricular conduction of electrical activation, including atrioventricular block, was observed in electrocardiograms of animals injected with the toxin. The toxin was also found to be toxic to cultured mouse heart cells. When it was added to the medium, the beating rhythm of the cultured heart cells increased temporarily and then soon stopped abruptly. The effect of the toxin on cultured mouse heart cells was blocked by preincubation of the toxin with a ganglioside mixture. From these results it is concluded that the thermostable direct hemolysin (lethal toxin) had cardiotoxic activity, and thus administration of the toxin causes rapid death of animals.
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- BERNHEIMER A. W., CARLSON A. S., FREEMAN E. B., KELLNER A. Loss of myocardial contractility induced in isolated mammalian hearts by streptolysin O. J Exp Med. 1956 Sep 1;104(3):361–373. doi: 10.1084/jem.104.3.361. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Goshima K. Further studies on preservation of the beating rhythm of myocardial cells in culture. Exp Cell Res. 1975 May;92(2):339–349. doi: 10.1016/0014-4827(75)90388-2. [DOI] [PubMed] [Google Scholar]
- Goshima K. Synchronized beating of and electrotonic transmission between myocardial cells mediated by heterotypic strain cells in monolayer culture. Exp Cell Res. 1969 Dec;58(2):420–426. doi: 10.1016/0014-4827(69)90523-0. [DOI] [PubMed] [Google Scholar]
- HALBERT S. P., BIRCHER R., DAHLE E. The analysis of streptococcal infections. V. Cardiotoxicity of streptolysin O for rabbits in vivo. J Exp Med. 1961 Apr 1;113:759–784. doi: 10.1084/jem.113.4.759. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Halpern B. N., Rahman S. Studies on the cardiotoxicity of streptolysin O. Br J Pharmacol Chemother. 1968 Mar;32(3):441–452. doi: 10.1111/j.1476-5381.1968.tb00445.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Honda T., Taga S., Takeda T., Hasibuan M. A., Takeda Y., Miwatani T. Identification of lethal toxin with the thermostable direct hemolysin produced by Vibrio parahaemolyticus, and some physicochemical properties of the purified toxin. Infect Immun. 1976 Jan;13(1):133–139. doi: 10.1128/iai.13.1.133-139.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kingdon G. C., Sword C. P. Cardiotoxic and Lethal Effects of Listeria monocytogenes Hemolysin. Infect Immun. 1970 Apr;1(4):373–379. doi: 10.1128/iai.1.4.373-379.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Takeda R., Honda T., Sakural J., Otomo N. Inhibition of hemolytic activity of the thermostable direct hemolysin of Vibrio parahaemolyticus by ganglioside. Infect Immun. 1975 Oct;12(4):931–933. doi: 10.1128/iai.12.4.931-933.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]