Abstract
The yield of purified type III polysaccharide of group B Streptococcus was significantly improved by modification of the growth medium. Culture of organisms in standard Todd-Hewitt broth resulted in acid accumulation during the exponential phase of growth and poor yield of type III polysaccharide when extracted from cells by washing with neutral buffer solution. By increasing the buffering capacity of the broth medium, acid accumulation was prevented, and the number of viable cells was increased at the stationary phase of growth. Further, by increasing the concentration of glucose in the buffered medium, the yield of type III polysaccharide was increased two to three times. Electron microscopic investigations of cells grown in the modified broth medium demonstrated a thicker microcapsule than was found in organisms grown in standard broth.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Baker C. J., Barrett F. F. Group B streptococcal infections in infants. The importance of the various serotypes. JAMA. 1974 Nov 25;230(8):1158–1160. [PubMed] [Google Scholar]
- Devoe I. W., Gilchrist J. E. Release of endotoxin in the form of cell wall blebs during in vitro growth of Neisseria meningitidis. J Exp Med. 1973 Nov 1;138(5):1156–1167. doi: 10.1084/jem.138.5.1156. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Freimer E. H. Type-specific polysaccharide antigens of group B streptococci. II. The chemical basis for serological specificity of the type II HCl antigen. J Exp Med. 1967 Mar 1;125(3):381–392. doi: 10.1084/jem.125.3.381. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kasper D. L., Winkelhake J. L., Zollinger W. D., Brandt B. L., Artenstein M. S. Immunochemical similarity between polysaccharide antigens of Escherichia coli 07: K1(L):NM and group B Neisseria meningitidis. J Immunol. 1973 Jan;110(1):262–268. [PubMed] [Google Scholar]
- Lancefield R. C., Freimer E. H. Type-specific polysaccharide antigens of group B streptococci. J Hyg (Lond) 1966 Jun;64(2):191–203. doi: 10.1017/s0022172400040456. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Luft J. H. Fine structures of capillary and endocapillary layer as revealed by ruthenium red. Fed Proc. 1966 Nov-Dec;25(6):1773–1783. [PubMed] [Google Scholar]
- Rowley D. Endotoxins and bacterial virulence. J Infect Dis. 1971 Mar;123(3):317–327. doi: 10.1093/infdis/123.3.317. [DOI] [PubMed] [Google Scholar]
- Swanson J., Gotschlich E. C. Electron microscopic studies on streptococci. II. Group A carbohydrate. J Exp Med. 1973 Jul 1;138(1):245–258. doi: 10.1084/jem.138.1.245. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wilkinson H. W., Eagon R. G. Type-specific antigens of group B type Ic streptococci. Infect Immun. 1971 Nov;4(5):596–604. doi: 10.1128/iai.4.5.596-604.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]