Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1976 Jan;13(1):211–220. doi: 10.1128/iai.13.1.211-220.1976

Variability in the structural polypeptides of herpes simplex virus 1 strains: potential application in molecular epidemiology.

L Pereira, E Cassai, R W Honess, B Roizman, M Terni, A Nahmias
PMCID: PMC420597  PMID: 175016

Abstract

This paper reports on the variability of structural polypeptides of 53 strains of herpes simplex virus 1 isolated from Italy, Uganda, South Africa, and various locations in the United States. Most strains were passaged a limited number of times at low multiplicity outside the human host; a few strains were characterized by numberous passages at variable multiplicities in cell culture and experimental animals. The acrylamide gel electrophoresis of polypeptides from purified virions revealed seven variable polypeptides. Virion polypeptides (VP) 7, 11, 13, 14, 15.2 and 23 were present in at least two isotypic forms characterized by fast and slow electrophoretic mobilities. VP8 could not be detected in three strains. In addition, VP13, 15.2, and 23 in some strains were either absent or comigrated with other polypeptides. A variety of tests showed that the variability in electrophoretic mobility of polypeptides was reproducible and could not be attributed to artifacts of purification, solubilization, or electrophoresis. Attempts to classify the strains on the basis of electrophoretic mobility of five or all seven variable polypeptides yielded 14 and 19 groups, respectively. The bulk of the strains (41 to 53) fell into six groups. Not all possible permutations of variable polypeptides were observed. Comparison of early and late passages of laboratory strains showed that in the few instances tested the variability could not be attributed to the propagation of the virus outside the human host. Clustering of strains on the basis of country of origin was not observed. Some clustering of isolates on the basis of site of isolation was observed, and the data do suggest that further analyses of isolates for evidence of a correlation between the site of localization on the human body and the structural polypeptides might be useful. Electrophoretic characterization of structural polypeptides has the potential of becoming a powerful tool for epidemiological studies of herpes simplex virus infections.

Full text

PDF
211

Images in this article

215Image
on p.215
216Image
on p.216
217Image
on p.217

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Antonelli A., Vignali C. Ricerche sulla localizzazione del virus dell'herpes simplex dopo guarigione delle recidive cutanee. Coltivazione di cellule della zona colpital da eruzione recidivante. Riv Ist Sieroter Ital. 1968 Jan-Feb;43(1):43–51. [PubMed] [Google Scholar]
  2. Becker W. B., Kipps A., McKenzie D. Disseminated herpes simplex virus infection. Its pathogenesis based on virological and pathological studies in 33 cases. Am J Dis Child. 1968 Jan;115(1):1–8. doi: 10.1001/archpedi.1968.02100010003001. [DOI] [PubMed] [Google Scholar]
  3. Bone D. R., Courtney R. J. A temperature-sensitive mutant of herpes simplex virus type 1 defective in the synthesis of the major capsid polypeptide. J Gen Virol. 1974 Jul;24(1):17–27. doi: 10.1099/0022-1317-24-1-17. [DOI] [PubMed] [Google Scholar]
  4. Cassai E. N., Sarmiento M., Spear P. G. Comparison of the virion proteins specified by herpes simplex virus types 1 and 2. J Virol. 1975 Nov;16(5):1327–1331. doi: 10.1128/jvi.16.5.1327-1331.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cassai E. N., Terni M., Califano A. Caratterizzazione di un virus dell'Herpes simplex isolato da un tumore labiale insorto sulla sede di recidive erpetiche. Tumori. 1974 Jul-Aug;60(4):325–336. doi: 10.1177/030089167406000408. [DOI] [PubMed] [Google Scholar]
  6. DULBECCO R., VOGT M. Plaque formation and isolation of pure lines with poliomyelitis viruses. J Exp Med. 1954 Feb;99(2):167–182. doi: 10.1084/jem.99.2.167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ejercito P. M., Kieff E. D., Roizman B. Characterization of herpes simplex virus strains differing in their effects on social behaviour of infected cells. J Gen Virol. 1968 May;2(3):357–364. doi: 10.1099/0022-1317-2-3-357. [DOI] [PubMed] [Google Scholar]
  8. Goodheart C. R., Plummer G., Waner J. L. Density difference of DNA of human herpes simplex viruses, types I and II. Virology. 1968 Jul;35(3):473–475. doi: 10.1016/0042-6822(68)90225-0. [DOI] [PubMed] [Google Scholar]
  9. HOGGAN M. D., ROIZMAN B. The isolation and properties of a variant of Herpes simplex producing multinucleated giant cells in monolayer cultures in the presence of antibody. Am J Hyg. 1959 Sep;70:208–219. doi: 10.1093/oxfordjournals.aje.a120071. [DOI] [PubMed] [Google Scholar]
  10. Hayward G. S., Frenkel N., Roizman B. Anatomy of herpes simplex virus DNA: strain differences and heterogeneity in the locations of restriction endonuclease cleavage sites. Proc Natl Acad Sci U S A. 1975 May;72(5):1768–1772. doi: 10.1073/pnas.72.5.1768. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Heine J. W., Honess R. W., Cassai E., Roizman B. Proteins specified by herpes simplex virus. XII. The virion polypeptides of type 1 strains. J Virol. 1974 Sep;14(3):640–651. doi: 10.1128/jvi.14.3.640-651.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Keller J. M., Spear P. G., Roizman B. Proteins specified by herpes simplex virus. 3. Viruses differing in their effects on the social behavior of infected cells specify different membrane glycoproteins. Proc Natl Acad Sci U S A. 1970 Apr;65(4):865–871. doi: 10.1073/pnas.65.4.865. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kieff E. D., Bachenheimer S. L., Roizman B. Size, composition, and structure of the deoxyribonucleic acid of herpes simplex virus subtypes 1 and 2. J Virol. 1971 Aug;8(2):125–132. doi: 10.1128/jvi.8.2.125-132.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Nahmias A. J., Chiang W. T., Del Buono I., Duffey A. Typing of Herpesvirus hominis strains by a direct immunofluorescent technique. Proc Soc Exp Biol Med. 1969 Oct;132(1):386–390. doi: 10.3181/00379727-132-34221. [DOI] [PubMed] [Google Scholar]
  15. Nahmias A. J., Roizman B. Infection with herpes-simplex viruses 1 and 2. II. N Engl J Med. 1973 Oct 4;289(14):719–725. doi: 10.1056/NEJM197310042891404. [DOI] [PubMed] [Google Scholar]
  16. ROIZMAN B., AURELIAN L. ABORTIVE INFECTION OF CANINE CELLS BY HERPES SIMPLEX VIRUS. I. CHARACTERIZATION OF VIRAL PROGENY FROM CO-OPERATIVE INFECTION WITH MUTANTS DIFFERING IN CAPACITY TO MULTIPLY IN CANINE CELLS. J Mol Biol. 1965 Mar;11:528–538. doi: 10.1016/s0022-2836(65)80008-0. [DOI] [PubMed] [Google Scholar]
  17. Rawls W. E., Laurel D., Melnick J. L., Glicksman J. M., Kaufman R. H. A search for viruses in smegma, premalignant and early malignant cervical tissues. The isolation of Herpesviruses with distinct antigenic properties. Am J Epidemiol. 1968 May;87(3):647–655. doi: 10.1093/oxfordjournals.aje.a120855. [DOI] [PubMed] [Google Scholar]
  18. Roizman B., Keller J. M., Spear P. G., Terni M., Nahmias A., Dowdle W. Variability, structural glycoproteins, and classification of herpes simplex viruses. Nature. 1970 Sep 19;227(5264):1253–1254. doi: 10.1038/2271253a0. [DOI] [PubMed] [Google Scholar]
  19. Roizman B., Spear P. G. Preparation of herpes simplex virus of high titer. J Virol. 1968 Jan;2(1):83–84. doi: 10.1128/jvi.2.1.83-84.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. SCHMIDT N. J., LENNETTE E. H. Colorimetric neutralization test for herpes simplex, with observations on neutralizing and complement-fixing antibody levels in human sera. J Immunol. 1961 Feb;86:137–145. [PubMed] [Google Scholar]
  21. Seth P., Rawls W. E., Duff R., Rapp F., Adam E., Melnick J. L. Antigenic differences between isolates of herpesvirus type 2. Intervirology. 1974;3(1-2):1–14. doi: 10.1159/000149738. [DOI] [PubMed] [Google Scholar]
  22. Spear P. G., Roizman B. Proteins specified by herpes simplex virus. V. Purification and structural proteins of the herpesvirion. J Virol. 1972 Jan;9(1):143–159. doi: 10.1128/jvi.9.1.143-159.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Tarro G., Sabin A. B. Virus-specific, labile, nonvirion antigen in herpesvirus-infected cells. Proc Natl Acad Sci U S A. 1970 Mar;65(3):753–760. doi: 10.1073/pnas.65.3.753. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Terni M., Bordin S. Osservazioni sull'effetto citopatico die virus dell'Herpes simplex. Riv Ist Sieroter Ital. 1967 Mar-Apr;42(2):83–93. [PubMed] [Google Scholar]
  25. Terni M., Roizman B. Variability of herpes simplex virus: isolation of two variants from simultaneous eruptions at different sites. J Infect Dis. 1970 Feb;121(2):212–216. doi: 10.1093/infdis/121.2.212. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES