Skip to main content
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1995 Apr 25;92(9):3859–3863. doi: 10.1073/pnas.92.9.3859

Degradation of the cleaved leader peptide of thiolase by a peroxisomal proteinase.

F Authier 1, J J Bergeron 1, W J Ou 1, R A Rachubinski 1, B I Posner 1, P A Walton 1
PMCID: PMC42061  PMID: 7731996

Abstract

A peroxisomal location for insulin-degrading enzyme (IDE) has been defined by confocal immunofluorescence microscopy of stably transfected CHO cells overexpressing IDE and digitonin-permeabilization studies in normal nontransfected fibroblasts. The functional significance of IDE in degrading cleaved leader peptides of peroxisomal proteins targeted by the type II motif was evaluated with a synthetic peptide corresponding to the type II leader peptide of prethiolase. The peptide effectively competed for degradation and cross-linking of the high-affinity substrate 125I-labeled insulin to IDE. Direct proteolysis of the leader peptide of prethiolase was confirmed by HPLC; degradation was inhibited by immunodepletion with an antibody to IDE. Phylogenetic analysis of proteinases related to IDE revealed sequence similarity to mitochondrial processing peptidases.

Full text

PDF
3859

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Affholter J. A., Hsieh C. L., Francke U., Roth R. A. Insulin-degrading enzyme: stable expression of the human complementary DNA, characterization of its protein product, and chromosomal mapping of the human and mouse genes. Mol Endocrinol. 1990 Aug;4(8):1125–1135. doi: 10.1210/mend-4-8-1125. [DOI] [PubMed] [Google Scholar]
  2. Alexson S. E., Fujiki Y., Shio H., Lazarow P. B. Partial disassembly of peroxisomes. J Cell Biol. 1985 Jul;101(1):294–304. doi: 10.1083/jcb.101.1.294. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Authier F., Rachubinski R. A., Posner B. I., Bergeron J. J. Endosomal proteolysis of insulin by an acidic thiol metalloprotease unrelated to insulin degrading enzyme. J Biol Chem. 1994 Jan 28;269(4):3010–3016. [PubMed] [Google Scholar]
  4. Baumeister H., Müller D., Rehbein M., Richter D. The rat insulin-degrading enzyme. Molecular cloning and characterization of tissue-specific transcripts. FEBS Lett. 1993 Feb 15;317(3):250–254. doi: 10.1016/0014-5793(93)81286-9. [DOI] [PubMed] [Google Scholar]
  5. Bodnar A. G., Rachubinski R. A. Cloning and sequence determination of cDNA encoding a second rat liver peroxisomal 3-ketoacyl-CoA thiolase. Gene. 1990 Jul 16;91(2):193–199. doi: 10.1016/0378-1119(90)90088-9. [DOI] [PubMed] [Google Scholar]
  6. Corpet F. Multiple sequence alignment with hierarchical clustering. Nucleic Acids Res. 1988 Nov 25;16(22):10881–10890. doi: 10.1093/nar/16.22.10881. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cousineau B., Cerpa C., Lefebvre J., Cedergren R. The sequence of the gene encoding elongation factor Tu from Chlamydia trachomatis compared with those of other organisms. Gene. 1992 Oct 12;120(1):33–41. doi: 10.1016/0378-1119(92)90006-b. [DOI] [PubMed] [Google Scholar]
  8. Finch P. W., Wilson R. E., Brown K., Hickson I. D., Emmerson P. T. Complete nucleotide sequence of the Escherichia coli ptr gene encoding protease III. Nucleic Acids Res. 1986 Oct 10;14(19):7695–7703. doi: 10.1093/nar/14.19.7695. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ichihara S., Beppu N., Mizushima S. Protease IV, a cytoplasmic membrane protein of Escherichia coli, has signal peptide peptidase activity. J Biol Chem. 1984 Aug 10;259(15):9853–9857. [PubMed] [Google Scholar]
  10. Ichihara S., Beppu N., Mizushima S. Protease IV, a cytoplasmic membrane protein of Escherichia coli, has signal peptide peptidase activity. J Biol Chem. 1984 Aug 10;259(15):9853–9857. [PubMed] [Google Scholar]
  11. Kuo W. L., Gehm B. D., Rosner M. R. Cloning and expression of the cDNA for a Drosophila insulin-degrading enzyme. Mol Endocrinol. 1990 Oct;4(10):1580–1591. doi: 10.1210/mend-4-10-1580. [DOI] [PubMed] [Google Scholar]
  12. Kuo W. L., Gehm B. D., Rosner M. R. Cloning and expression of the cDNA for a Drosophila insulin-degrading enzyme. Mol Endocrinol. 1990 Oct;4(10):1580–1591. doi: 10.1210/mend-4-10-1580. [DOI] [PubMed] [Google Scholar]
  13. Kuo W. L., Gehm B. D., Rosner M. R., Li W., Keller G. Inducible expression and cellular localization of insulin-degrading enzyme in a stably transfected cell line. J Biol Chem. 1994 Sep 9;269(36):22599–22606. [PubMed] [Google Scholar]
  14. Meulenberg J. J., Sellink E., Riegman N. H., Postma P. W. Nucleotide sequence and structure of the Klebsiella pneumoniae pqq operon. Mol Gen Genet. 1992 Mar;232(2):284–294. doi: 10.1007/BF00280008. [DOI] [PubMed] [Google Scholar]
  15. Ossendorp B. C., Wirtz K. W. The non-specific lipid-transfer protein (sterol carrier protein 2) and its relationship to peroxisomes. Biochimie. 1993;75(3-4):191–200. doi: 10.1016/0300-9084(93)90077-6. [DOI] [PubMed] [Google Scholar]
  16. Ou W. J., Ito A., Okazaki H., Omura T. Purification and characterization of a processing protease from rat liver mitochondria. EMBO J. 1989 Sep;8(9):2605–2612. doi: 10.1002/j.1460-2075.1989.tb08400.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rawlings N. D., Barrett A. J. Homologues of insulinase, a new superfamily of metalloendopeptidases. Biochem J. 1991 Apr 15;275(Pt 2):389–391. doi: 10.1042/bj2750389. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Silver P., Watts C., Wickner W. Membrane assembly from purified components. I. Isolated M13 procoat does not require ribosomes or soluble proteins for processing by membranes. Cell. 1981 Aug;25(2):341–345. doi: 10.1016/0092-8674(81)90052-0. [DOI] [PubMed] [Google Scholar]
  19. Subramani S. Protein import into peroxisomes and biogenesis of the organelle. Annu Rev Cell Biol. 1993;9:445–478. doi: 10.1146/annurev.cb.09.110193.002305. [DOI] [PubMed] [Google Scholar]
  20. Subramani S. Protein import into peroxisomes and biogenesis of the organelle. Annu Rev Cell Biol. 1993;9:445–478. doi: 10.1146/annurev.cb.09.110193.002305. [DOI] [PubMed] [Google Scholar]
  21. Swinkels B. W., Gould S. J., Bodnar A. G., Rachubinski R. A., Subramani S. A novel, cleavable peroxisomal targeting signal at the amino-terminus of the rat 3-ketoacyl-CoA thiolase. EMBO J. 1991 Nov;10(11):3255–3262. doi: 10.1002/j.1460-2075.1991.tb04889.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Van Veldhoven P. P., Just W. W., Mannaerts G. P. Permeability of the peroxisomal membrane to cofactors of beta-oxidation. Evidence for the presence of a pore-forming protein. J Biol Chem. 1987 Mar 25;262(9):4310–4318. [PubMed] [Google Scholar]
  23. Waechter F., Bentley P., Bieri F., Stäubli W., Völkl A., Fahimi H. D. Epoxide hydrolase activity in isolated peroxisomes of mouse liver. FEBS Lett. 1983 Jul 25;158(2):225–228. doi: 10.1016/0014-5793(83)80583-3. [DOI] [PubMed] [Google Scholar]
  24. Waechter F., Bentley P., Bieri F., Stäubli W., Völkl A., Fahimi H. D. Epoxide hydrolase activity in isolated peroxisomes of mouse liver. FEBS Lett. 1983 Jul 25;158(2):225–228. doi: 10.1016/0014-5793(83)80583-3. [DOI] [PubMed] [Google Scholar]
  25. Walton P. A., Gould S. J., Feramisco J. R., Subramani S. Transport of microinjected proteins into peroxisomes of mammalian cells: inability of Zellweger cell lines to import proteins with the SKL tripeptide peroxisomal targeting signal. Mol Cell Biol. 1992 Feb;12(2):531–541. doi: 10.1128/mcb.12.2.531. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Wanders R. J., Kos M., Roest B., Meijer A. J., Schrakamp G., Heymans H. S., Tegelaers W. H., van den Bosch H., Schutgens R. B., Tager J. M. Activity of peroxisomal enzymes and intracellular distribution of catalase in Zellweger syndrome. Biochem Biophys Res Commun. 1984 Sep 28;123(3):1054–1061. doi: 10.1016/s0006-291x(84)80240-5. [DOI] [PubMed] [Google Scholar]
  27. Wanders R. J., Kos M., Roest B., Meijer A. J., Schrakamp G., Heymans H. S., Tegelaers W. H., van den Bosch H., Schutgens R. B., Tager J. M. Activity of peroxisomal enzymes and intracellular distribution of catalase in Zellweger syndrome. Biochem Biophys Res Commun. 1984 Sep 28;123(3):1054–1061. doi: 10.1016/s0006-291x(84)80240-5. [DOI] [PubMed] [Google Scholar]
  28. Witte C., Jensen R. E., Yaffe M. P., Schatz G. MAS1, a gene essential for yeast mitochondrial assembly, encodes a subunit of the mitochondrial processing protease. EMBO J. 1988 May;7(5):1439–1447. doi: 10.1002/j.1460-2075.1988.tb02961.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. van den Bosch H., Schutgens R. B., Wanders R. J., Tager J. M. Biochemistry of peroxisomes. Annu Rev Biochem. 1992;61:157–197. doi: 10.1146/annurev.bi.61.070192.001105. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES