Skip to main content

Some NLM-NCBI services and products are experiencing heavy traffic, which may affect performance and availability. We apologize for the inconvenience and appreciate your patience. For assistance, please contact our Help Desk at info@ncbi.nlm.nih.gov.

Infection and Immunity logoLink to Infection and Immunity
. 1976 Feb;13(2):337–344. doi: 10.1128/iai.13.2.337-344.1976

Evidence for a one-hit theory in the immune bactericidal reaction and demonstration of a multi-hit response for hemolysis by streptolysin O and Clostridium perfringens theta-toxin.

K Inoue, Y Akiyama, T Kinoshita, Y Higashi, T Amano
PMCID: PMC420617  PMID: 177364

Abstract

An analytical method was developed for estimating the number of hits necessary to lyse or kill cells in which various concentrations of the cells are treated with a constant amount of the lytic or killing agent in a constant reaction volume. The reaction may be due to a single-component agent or occur by a sequential chain of reactions due to a multi-component agent, even including side, abortive, or counter-reactions. It was clearly shown by this method that immune bactericidal reactions followed a one-hit theory. It was shown by this method that streptolysin O required four or five hits for hemolysis and Clostridium perfringens theta-toxin required two hits. These results were confirmed by both logarithmic dose-response and survival analyses. It was also shown that streptolysin O and theta-toxin can act complementarily on accumulation of the hits for hemolysis.

Full text

PDF
337

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alouf J. E., Raynaud M. Action de la streptolysine O sur les membranes cellulaires . II.--Cinétique de la lyse érythrocytaire. Ann Inst Pasteur (Paris) 1968 Jul;115(1):97–121. [PubMed] [Google Scholar]
  2. ECHOLS H., GAREN A., GAREN S., TORRIANI A. Genetic control of repression of alkaline phosphatase in E. coli. J Mol Biol. 1961 Aug;3:425–438. doi: 10.1016/s0022-2836(61)80055-7. [DOI] [PubMed] [Google Scholar]
  3. Higashi Y., Chazono M., Inoue K., Yanagase Y., Amano T. Complementation of theta-toxinogenicity between mutants of two groups of Clostridium perfringens. Biken J. 1973 Mar;16(1):1–9. [PubMed] [Google Scholar]
  4. Inoue K., Takamizawa A., Yano K., Amano T. Chemical studies on damages of Escherichia coli by the immune bactericidal reaction. I. Release and degradation of phospholipids from damaged bacteria. Biken J. 1974 Dec;17(4):127–134. [PubMed] [Google Scholar]
  5. Kanbayashi Y., Hotta M., Koyama J. Kinetic study on streptolysin O. J Biochem. 1972 Feb;71(2):227–237. doi: 10.1093/oxfordjournals.jbchem.a129759. [DOI] [PubMed] [Google Scholar]
  6. WEBBER B. B., DESERRES F. J. INDUCTION KINETICS AND GENETIC ANALYSIS OF X-RAY-INDUCED MUTATIONS IN THE AD-3 REGION OF NEUROSPORA CRASSA. Proc Natl Acad Sci U S A. 1965 Feb;53:430–437. doi: 10.1073/pnas.53.2.430. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES