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. 1976 Feb;13(2):417–424. doi: 10.1128/iai.13.2.417-424.1976

Influenza infection in ferrets: role of serum antibody in protection and recovery.

P A Small Jr, R H Waldman, J C Bruno, G E Gifford
PMCID: PMC420629  PMID: 1262059

Abstract

The passive administration of ferret antiserum to Ao (H0N1) influenza virus failed to protect the recipient ferrets from subsequent infection with homologous virus. This susceptibility to infection was observed even when the passively acquired serum hemagglutination inhibition (HI) titer was similar to peak convalescent titers. It is therefore concluded that serum antibody alone is probably not a major factor in the prevention of influenza infection. This does not rule out a possible role for serum antibody in prevention of illness. Subsequent to infection, ferrets that had received passive antisera failed to develop high levels of serum HI antibody. In fact, many had no detectable serum antibody (less than 1:8). These animals shed virus for periods of time quite similar to those of infected control animals, which did develope serum antibody. From these data it was concluded that detectable serum HI antibody does not play a significant role in the recovery of ferrets from influenza infection. Interferon was present in high concentrations in the secretions a few days prior to cessation of virus shedding, but it is not clear whether this was the cause of the recovery or merely a concomitant event. Twenty-one days after initial infection two-thirds of the ferrets that had received passive antibody and all control animals were immune to reinfection with the homologous influenza virus. Since the former group had little or no detectable serum HI antibody but most members were immune, there must be some other host mechanism to account for the immunity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARON S., ISAACS A. Mechanism of recovery from viral infection in the chick embryo. Nature. 1961 Jul 1;191:97–98. doi: 10.1038/191097a0. [DOI] [PubMed] [Google Scholar]
  2. Barber W. H., Small P. A., Jr Dissemination of influenza virus between anatomically isolated sites in ferrets. Infect Immun. 1974 Mar;9(3):530–533. doi: 10.1128/iai.9.3.530-533.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Beare A. S., Hobson D., Reed S. E., Tyrrell D. A. A comparison of live and killed influenza-virus vaccines. Report to the Medical Research Council's Committee on Influenza and other Respiratory Virus Vaccines. Lancet. 1968 Aug 24;2(7565):418–422. doi: 10.1016/s0140-6736(68)90463-7. [DOI] [PubMed] [Google Scholar]
  4. Beare A. S., Tyrrell D. A., Hobson D., Howells C. H., Pereira M. S., Pollock T. M., Tyler L. E. Live influenza B vaccine in volunteers. A report to the Medical Research Council by their Committee on Influenza and Other Respiratory Virus Vaccines. J Hyg (Lond) 1969 Mar;67(1):1–11. doi: 10.1017/s002217240004136x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. CHANOCK R. M., PARROTT R. H., COOK K., ANDREWS B. E., BELL J. A., REICHELDERFER T., KAPIKIAN A. Z., MASTROTA F. M., HUEBNER R. J. Newly recognized myxoviruses from children with respiratory disease. N Engl J Med. 1958 Jan 30;258(5):207–213. doi: 10.1056/NEJM195801302580502. [DOI] [PubMed] [Google Scholar]
  6. Edmondson W. P., Jr, Rothenberg R., White P. W., Gwaltney J. M., Jr A comparison of subcutaneous, nasal, and combined influenza vaccination. II. Protection against natural challenge. Am J Epidemiol. 1971 Jun;93(6):480–486. doi: 10.1093/oxfordjournals.aje.a121282. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. FAZEKAS de ST GROTH S., DONNELLEY M. Studies in experimental immunology of influenza. IV. The protective value of active immunization. Aust J Exp Biol Med Sci. 1950 Jan;28(1):61–75. doi: 10.1038/icb.1950.5. [DOI] [PubMed] [Google Scholar]
  8. FAZEKAS de ST GROTH S. Influenza: a study in mice. Lancet. 1950 Jun 17;1(6616):1101–1105. doi: 10.1016/s0140-6736(50)91904-0. [DOI] [PubMed] [Google Scholar]
  9. GRESSER I., DULL H. B. A VIRUS INHIBITOR IN PHARYNGEAL WASHINGS FROM PATIENTS WITH INFLUENZA. Proc Soc Exp Biol Med. 1964 Jan;115:192–196. doi: 10.3181/00379727-115-28867. [DOI] [PubMed] [Google Scholar]
  10. Haff R. F., Schriver P. W., Engle C. G., Stewart R. C. Pathogenesis of influenza in ferrets. I. Tissue and blood manifestations of disease. J Immunol. 1966 Apr;96(4):659–667. [PubMed] [Google Scholar]
  11. Haff R. F., Schriver P. W., Stewart R. C. Pathogenesis of influenza in ferrets: nasal manifestations of disease. Br J Exp Pathol. 1966 Oct;47(5):435–444. [PMC free article] [PubMed] [Google Scholar]
  12. Jao R. L., Wheelock E. F., Jackson G. G. Production of interferon in volunteers infected with Asian influenza. J Infect Dis. 1970 Apr;121(4):419–426. doi: 10.1093/infdis/121.4.419. [DOI] [PubMed] [Google Scholar]
  13. LIU C. Studies of influenza infection in ferrets by means of fluorescein-labelled antibody. I. The pathogenesis and diagnosis of the disease. J Exp Med. 1955 Jun 1;101(6):665–676. doi: 10.1084/jem.101.6.665. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. LOOSLI C. G., HAMRE D., BERLIN B. S. Air-borne influenza virus A infections in immunized animals. Trans Assoc Am Physicians. 1953;66:222–230. [PubMed] [Google Scholar]
  15. Lien K. S., Jacobs J., Marcus E. A., Strik R. V. The protective effect of intranasal immunization with inactivated influenza virus vaccine. Postgrad Med J. 1973 Mar;49(569):175–179. [PMC free article] [PubMed] [Google Scholar]
  16. Liew F. Y., Parish C. R. Regulation of the immune response by antibody. I. Suppression of antibody formation and concomitant enhancement of cell-mediated immunity by passive antibody. Cell Immunol. 1972 May;4(1):66–85. doi: 10.1016/0008-8749(72)90006-8. [DOI] [PubMed] [Google Scholar]
  17. Marois P., Boudreault A., DiFranco E., Pavilanis V. Response of ferrets and monkeys to intranasal infection with human, equine and avian influenza viruses. Can J Comp Med. 1971 Jan;35(1):71–76. [PMC free article] [PubMed] [Google Scholar]
  18. McLaren C., Potter C. W., Jennings R. Immunity to influenza in ferrets. X. Intranasal immunization of ferrets with inactivated influenza A virus vaccines. Infect Immun. 1974 Jun;9(6):985–990. doi: 10.1128/iai.9.6.985-990.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Morris J. A., Kasel J. A., Saglam M., Knight V., Loda F. A. Immunity to influenza to antibody levels. N Engl J Med. 1966 Mar 10;274(10):527–535. doi: 10.1056/NEJM196603102741001. [DOI] [PubMed] [Google Scholar]
  20. PRZESMYCKI F., SAWICKI L., DOBROWOLSKA H. Vaccination against influenza in Poland, 1953/56. Bull World Health Organ. 1959;20(2-3):333–353. [PMC free article] [PubMed] [Google Scholar]
  21. Potter C. W., Oxford J. S., Shore S. L., McLaren C., Stuart-Harris C. Immunity to influenza in ferrets. I. Response to live and killed virus. Br J Exp Pathol. 1972 Apr;53(2):153–167. [PMC free article] [PubMed] [Google Scholar]
  22. Potter C. W., Shore S. L., McLaren C. Immunity to influenza in ferrets. 3. Proteins in nasal secretions. J Infect Dis. 1972 Oct;126(4):387–393. doi: 10.1093/infdis/126.4.387. [DOI] [PubMed] [Google Scholar]
  23. Rouse B. T., Ditchfield W. J. The response of ponies to Myxovirus influenzae A-equi 2. I. Serum and nasal antibody titres following exposure. Can J Comp Med. 1970 Jan;34(1):1–5. [PMC free article] [PubMed] [Google Scholar]
  24. SEVER J. L. Application of a microtechnique to viral serological investigations. J Immunol. 1962 Mar;88:320–329. [PubMed] [Google Scholar]
  25. SUGG J. Y., NAGAKI D. Tissue tropism of a strain of influenza A virus in ferrets. J Immunol. 1955 Jan;74(1):46–50. [PubMed] [Google Scholar]
  26. Shore S. L., Potter C. W., McLaren C. Immunity to influenza in ferrets. IV. Antibody in nasal secretions. J Infect Dis. 1972 Oct;126(4):394–400. doi: 10.1093/infdis/126.4.394. [DOI] [PubMed] [Google Scholar]
  27. Stuart-Harris C. Influenza--the problems and the future. Med J Aust. 1973 Jun 2;(Suppl):42–47. [PubMed] [Google Scholar]
  28. Uhr J. W., Möller G. Regulatory effect of antibody on the immune response. Adv Immunol. 1968;8:81–127. doi: 10.1016/s0065-2776(08)60465-4. [DOI] [PubMed] [Google Scholar]
  29. Virelizier J. L. Host defenses against influenza virus: the role of anti-hemagglutinin antibody. J Immunol. 1975 Aug;115(2):434–439. [PubMed] [Google Scholar]
  30. Waldman R. H., Bond J. O., Levitt L. P., Hartwig E. C., Prather E. C., Baratta R. L., Neill J. S., Small P. A., Jr An evaluation of influenza immunization: influence of route of administration and vaccine strain. Bull World Health Organ. 1969;41(3):543–548. [PMC free article] [PubMed] [Google Scholar]
  31. Waldman R. H., Henney C. S. Cell-mediated immunity and antibody responses in the respiratory tract after local and systemic immunization. J Exp Med. 1971 Aug 1;134(2):482–494. doi: 10.1084/jem.134.2.482. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Waldman R. H., Mann J. J., Small P. A., Jr Immunization against influenza. Prevention of illness in man by aerosolized inactivated vaccine. JAMA. 1969 Jan 20;207(3):520–524. doi: 10.1001/jama.207.3.520. [DOI] [PubMed] [Google Scholar]

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