Abstract
The induced auxotrophy for p-aminobenzoic acid (PABA) resulted in a complete loss of virulence of Aspergillus fumigatus for normal as well as cortisone-treated mice. The PABA-requiring mutant of A. fumigatus survived in vivo for 4 to 7 days without causing any infection. However, it showed conditional virulence in animals receiving PABA in very small quantities. Repeated inoculations of the viable spores of the avirulent mutant strain gave favorable results in building immunity against intravenous challenge of the virulent strain. The immunogenicity of the PABA-requiring mutant was comparable with that of a wild strain of the fungus in agar gel double-diffusion tests using clinical and hyperimmune sera and in skin tests on patients with allergic bronchopulmonary aspergillosis.
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- Azuma I., Kimura H., Hirao F., Tsubura E., Yamamura Y. Biochemical and immunological studies on aspergillus. II. Immunological properties of protein and polysaccharide fractions obtained from Aspergillus fumigatus. Mycopathol Mycol Appl. 1969 May 23;37(3):289–303. doi: 10.1007/BF02051363. [DOI] [PubMed] [Google Scholar]
- BURROWS T. W. Biochemical properties of virulent and avirulent strains of bacteria: Salmonella typhosa and Pasteurella pestis. Ann N Y Acad Sci. 1960 Nov 21;88:1125–1135. doi: 10.1111/j.1749-6632.1960.tb20102.x. [DOI] [PubMed] [Google Scholar]
- BUXTON E. W. Heterokaryosis and parasexual recombination in pathogenic strains of Fusarium oxysporum. J Gen Microbiol. 1956 Aug;15(1):133–139. doi: 10.1099/00221287-15-1-133. [DOI] [PubMed] [Google Scholar]
- Chung K. J., Hill W. B. Studies on the pink, adenine-deficient strains of Candida albicans. I. Cultural and morphological characteristics. Sabouraudia. 1970 May;8(1):48–59. [PubMed] [Google Scholar]
- Donkersloot J. A., Mateles R. I. Enrichment of auxotrophic mutants of Aspergillus flavus by tritium suicide. J Bacteriol. 1968 Nov;96(5):1551–1555. doi: 10.1128/jb.96.5.1551-1555.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GARBER E. D. The host as a growth medium. Ann N Y Acad Sci. 1960 Nov 21;88:1187–1194. doi: 10.1111/j.1749-6632.1960.tb20108.x. [DOI] [PubMed] [Google Scholar]
- Ivánovics G., Marjai E., Dobozy A. The growth of purine mutants of Bacillus anthracis in the body of the mouse. J Gen Microbiol. 1968 Sep;53(2):147–162. doi: 10.1099/00221287-53-2-147. [DOI] [PubMed] [Google Scholar]
- PANOS C., AJL S. J. METABOLISM OF MICROORGANISMS AS RELATED TO THEIR PATHOGENICITY. Annu Rev Microbiol. 1963;17:297–328. doi: 10.1146/annurev.mi.17.100163.001501. [DOI] [PubMed] [Google Scholar]
- PAPPAGIANIS D., LEVINE H. B., SMITH C. E., BERMAN R. J., KOBAYASHI G. S. Immunization of mice with viable Cocidioides immitis. J Immunol. 1961 Jan;86:28–34. [PubMed] [Google Scholar]
- Purnell D. M. The effects of specific auxotrophic mutations on the virulence of Aspergillus nidulans for mice. Mycopathol Mycol Appl. 1973 Jul 31;50(3):195–203. doi: 10.1007/BF02053368. [DOI] [PubMed] [Google Scholar]
- ROBERTS C. F. A replica plating technique for the isolation of nutritionally exacting mutants of a filamentous fungus (Aspergillus nidulans). J Gen Microbiol. 1959 Jun;20(3):540–548. doi: 10.1099/00221287-20-3-540. [DOI] [PubMed] [Google Scholar]
- STROMNAES O., GARBER E. D. Heterocaryosis and the parasexual cycle in Aspergillus fumigatus. Genetics. 1963 May;48:653–662. doi: 10.1093/genetics/48.5.653. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sandhu D. K., Sandhu R. S., Damodaran V. N., Randhawa H. S. Pathogenicity of Aspergillus species isolated from human respiratory tract. Indian J Chest Dis. 1969 Jul;11(3):115–121. [PubMed] [Google Scholar]
- Walch H. A., Kalvoda A. Immunization of mice with induced mutants of Coccidioides immitis. I. Characterization of mutants and preliminary studies of their use as viable vaccines. Sabouraudia. 1971 Nov;9(3):173–184. [PubMed] [Google Scholar]