Abstract
This investigation examined the theoretical and practical parameters requisite to preparatory istachophoretic separation of blastomycin purified derivative. It resulted in a relatively simple, two-step procedure for preparation of blastomycin antigens in milligram quantitites that exhibited sensitivity and specificity in experimentally infected guinea pigs. Analysis of the nine isotachophoretic fractions for skin test sensitivity and specificity provided some insight into the generally accepted unreliability of blastomycin when used for immunological evaluation.
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- Catsimpoolas N. Micro isoelectric focusing in polyacrylamide gel columns. Anal Biochem. 1968 Dec;26(3):480–482. doi: 10.1016/0003-2697(68)90219-4. [DOI] [PubMed] [Google Scholar]
- Cox R. A., Larsh H. W. Yeast- and mycelial-phase antigens of Blastomyces dermatitidis: comparison using disc gel electrophoresis. Infect Immun. 1974 Jul;10(1):48–53. doi: 10.1128/iai.10.1.48-53.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Furcolow M. L., Chick E. W., Busey J. F., Menges R. W. Prevalence and incidence studies of human and canine blastomycosis. 1. Cases in the United States, 1885-1968. Am Rev Respir Dis. 1970 Jul;102(1):60–67. doi: 10.1164/arrd.1970.102.1.60. [DOI] [PubMed] [Google Scholar]
- Goodman N. L., Spouse R. F., Larsh H. W. Histoplasmin potency as affected by culture age. Sabouraudia. 1968 Oct;6(4):273–284. doi: 10.1080/00362176885190551. [DOI] [PubMed] [Google Scholar]
- Griffith A., Catsimpoolas N. General aspects of analytical isotachophoresis of proteins in polyacrylamide gels. Anal Biochem. 1972 Jan;45(1):192–201. doi: 10.1016/0003-2697(72)90019-x. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- MENGES R. W., FURCOLOW M. L., SELBY L. A., ELLIS H. R., HABERMANN R. T. CLINICAL AND EPIDEMIOLOGIC STUDIES ON SEVENTY-NINE CANINE BLASTOMYCOSIS CASES IN ARKANSAS. Am J Epidemiol. 1965 Mar;81:164–179. doi: 10.1093/oxfordjournals.aje.a120505. [DOI] [PubMed] [Google Scholar]