Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1976 Mar;13(3):825–829. doi: 10.1128/iai.13.3.825-829.1976

Erythrocyte antinuclear antibodies in sera of chickens hyperimmunized with group A streptococcal vaccine.

M I Luster, G A Leslie
PMCID: PMC420684  PMID: 773827

Abstract

Chickens hyperimmunized with group A streptococcal vaccine often synthesize high levels of antibody to group A streptococcal carbohydrate (SACHO). Indirect immunofluorescent analysis revealed that sera of these chickens also contain antinuclear antibodies capable of reacting with chicken erythrocyte nuclei (EANA) at titers up to 2,560. Removal of the anti-SACHO antibodies from hyperimmune serum did not significantly reduce EANA titers, indicating that anti-SACHO antibodies are not responsible for the EANA reactions. The time course for antibody development as well as the immunoglobulin class distribution of EANA and anti-SACHO antibodies were very similar. Localization of EANA activity to Fab fragments indicated that the immunfluorescent reaction represents an antigen-antibody reaction. Findings point out an important consequence of using chicken immunoglobulin Y in immunofluorescent assays. The purified Fc fragment of immunoglobulin Y is cytophilic for the substrate when tested at high concentrations, which suggests that some cytophilic immunoglobulins are involved in the reaction.

Full text

PDF
825

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Albini B., Wick G. Erythrocyte-specific anti-nuclear factors in chickens of the obese strain (OS). Immunology. 1971 Dec;21(6):957–966. [PMC free article] [PubMed] [Google Scholar]
  2. Braun D. G., Krause R. M. Die Morphologie von Kaninchenorganen nach Immunisierung mit Streptokokken-Vaccine. Z Immunitatsforsch Allerg Klin Immunol. 1969 Dec;139(1):104–117. [PubMed] [Google Scholar]
  3. Dreesman G., Benedict A. A. Properties of papain-digested chicken 7 S gamma-globulin. J Immunol. 1965 Nov;95(5):855–866. [PubMed] [Google Scholar]
  4. Friou G. J. Antinuclear antibodies: diagnostic significance and methods. Arthritis Rheum. 1967 Apr;10(2):151–159. doi: 10.1002/art.1780100210. [DOI] [PubMed] [Google Scholar]
  5. Ginsburg I. Mechanisms of cell and tissue injury induced by group A streptococci: relation to poststreptococcal sequelae. J Infect Dis. 1972 Oct;126(4):419–456. doi: 10.1093/infdis/126.4.419. [DOI] [PubMed] [Google Scholar]
  6. Iványi J. Cytophilic antibodies in passive aibody induced immune suppression or enhancement. Nature. 1970 May 9;226(5245):550–551. doi: 10.1038/226550b0. [DOI] [PubMed] [Google Scholar]
  7. Jones J. M., Schwab J. H. Immune response to cell wall and tissue antigens in rabbits injured with streptococcal cell wall fragments. Int Arch Allergy Appl Immunol. 1970;39(4):445–448. doi: 10.1159/000230373. [DOI] [PubMed] [Google Scholar]
  8. Kubo R. T., Benedict A. A. Unusual conditions for crystallization of the Fc fragment of chicken IgG. J Immunol. 1969 Jun;102(6):1523–1525. [PubMed] [Google Scholar]
  9. Leslie G. A., Benedict A. A. Structural and antigenic relationships between avian immunoglobulins. II. Properties of papain- and pepsin-digested chicken, pheasant and quail IgG-immunoglobulins. J Immunol. 1970 Apr;104(4):810–817. [PubMed] [Google Scholar]
  10. Leslie G. A., Carwile H. F. Immune response of rats to group A streptococcal vaccine. Infect Immun. 1973 May;7(5):781–785. doi: 10.1128/iai.7.5.781-785.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Leslie G. A., Clem L. W. Chicken antibodies to group A streptococcal carbohydrate. J Immunol. 1973 Jan;110(1):191–196. [PubMed] [Google Scholar]
  12. Leslie G. A., Clem L. W. Phylogen of immunoglobulin structure and function. 3. Immunoglobulins of the chicken. J Exp Med. 1969 Dec 1;130(6):1337–1352. doi: 10.1084/jem.130.6.1337. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Leslie G. A., Hattier P. Detection and quantitation of chicken antibodies to streptococcal carbohydrate by high-salt reverse radial immunodiffusion. J Immunol Methods. 1974 Jan;3(1):53–58. doi: 10.1016/0022-1759(74)90033-7. [DOI] [PubMed] [Google Scholar]
  14. Leslie G. A., Martin L. N. Studies on the secretory immunologic system of fowl. 3. Serum and secretory IgA of the chicken. J Immunol. 1973 Jan;110(1):1–9. [PubMed] [Google Scholar]
  15. MAKINODAN T., WOLFE H. R., GOODMAN M., RUTH R. Precipitin production in chickens. VII. The relation between circulating antibody and anaphylactic shock. J Immunol. 1952 Mar;68(3):219–226. [PubMed] [Google Scholar]
  16. Martin L. N., Leslie G. A. IgM-forming cells as the immediate precursor of IgA-producing cells during ontogeny of the immunoglobulin-producing system of the chicken. J Immunol. 1974 Jul;113(1):120–126. [PubMed] [Google Scholar]
  17. Ohanian S. H., Schwab J. H. Persistence of group a streptococcal cell walls related to chronic inflammation of rabbit dermal connective tissue. J Exp Med. 1967 Jun 1;125(6):1137–1148. doi: 10.1084/jem.125.6.1137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Osterland C. K., Miller E. J., Karakawa W. W., Krause R. M. Characteristics of streptococcal group-specific antibody isolated from hyperimmune rabbits. J Exp Med. 1966 Apr 1;123(4):599–614. doi: 10.1084/jem.123.4.599. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. PORTER R. R. The hydrolysis of rabbit y-globulin and antibodies with crystalline papain. Biochem J. 1959 Sep;73:119–126. doi: 10.1042/bj0730119. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Rotta J., Krause R. M., Lancefield R. C., Everly W., Lackland H. New approaches for the laboratory recognition of M types of group A streptococci. J Exp Med. 1971 Nov 1;134(5):1298–1315. doi: 10.1084/jem.134.5.1298. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Stankus R. P., Leslie G. A. Nonprecipitating and electrophoretically restricted antibodies to carbohydrate of group A Streptococcus in the rat. J Infect Dis. 1974 Aug;130(2):169–173. doi: 10.1093/infdis/130.2.169. [DOI] [PubMed] [Google Scholar]
  22. The T. H., Feltkamp T. E. Conjugation of fluorescein isothiocyanate to antibodies. II. A reproducible method. Immunology. 1970 Jun;18(6):875–881. [PMC free article] [PubMed] [Google Scholar]
  23. Thivolet J., Monier J. C., Ruel J. P., Richard M. H. Antinuclear autoantibodies in Swiss mice thymectomized at birth. Nature. 1967 Jun 10;214(5093):1134–1136. doi: 10.1038/2141134a0. [DOI] [PubMed] [Google Scholar]
  24. ten Veen J. H., Feltkamp T. E. Formalinized chicken red cell nuclei as a simple antigen for standardized antinuclear factor determination. Clin Exp Immunol. 1969 Dec;5(6):673–678. [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES