Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1976 Apr;13(4):1258–1265. doi: 10.1128/iai.13.4.1258-1265.1976

Major outer membrane protein of Campylobacter fetus: physical and immunological characterization.

E C McCoy, H A Wiltberger, J Winter
PMCID: PMC420747  PMID: 819374

Abstract

The outer membrane proteins of Campylobacter fetus have been isolated by extraction of cell envelopes both in Triton X-100 and ethylenediaminetetraacetate (EDTA) and in sodium dodecyl sulfate (SDS). Each method yielded a major protein component, which migrated identically in SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and proved comparable in electrophoretic and molecular weight characteristics to the analagous protein from Escherichia coli. In addition, the surface of SDS-extracted C. fetus cells displayed a subunit structure similar to that observed in E. coli. The major envelope protein isolated with SDS appeared antigenically identical with one of the proteins isolated with Trition-EDTA on the basis of immunodiffusion reactions with specific antisera. Antibodies directed to the major envelope protein were not reactive in agglutination, immobilization, bactericidal, or opsonization reactions. Strains of C. fetus belonging to each of the three O serotypes possessed major envelope proteins comparable in SDS-PAGE but distinguished antigencally in a fashion paralleling the O serotype classification.

Full text

PDF
1258

Images in this article

1261Image
on p.1261
1261Image
on p.1261
1262Image
on p.1262
1263Image
on p.1263
1264Image
on p.1264

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berg R. L., Jutila J. W., Firehammer B. D. A revised classification of Vibrio fetus. Am J Vet Res. 1971 Jan;32(1):11–22. [PubMed] [Google Scholar]
  2. Braun V., Rehn K. Chemical characterization, spatial distribution and function of a lipoprotein (murein-lipoprotein) of the E. coli cell wall. The specific effect of trypsin on the membrane structure. Eur J Biochem. 1969 Oct;10(3):426–438. doi: 10.1111/j.1432-1033.1969.tb00707.x. [DOI] [PubMed] [Google Scholar]
  3. Braun V., Wolff H. The murein-lipoprotein linkage in the cell wall of Escherichia coli. Eur J Biochem. 1970 Jun;14(2):387–391. doi: 10.1111/j.1432-1033.1970.tb00301.x. [DOI] [PubMed] [Google Scholar]
  4. Cho C. T., Feng K. K. Non-immunological precipitation of serum by sodium dodecyl sulfate in agar diffusion. Appl Microbiol. 1974 Oct;28(4):557–560. doi: 10.1128/am.28.4.557-560.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Duncan J. R., Wilkie B. N., Hiestand F., Winter A. J. The serum and secretory immunoglobulins of cattle: characterization and quantitation. J Immunol. 1972 Apr;108(4):965–976. [PubMed] [Google Scholar]
  6. Frasch C. E., Gotschlich E. C. An outer membrane protein of Neisseria meningitidis group B responsible for serotype specificity. J Exp Med. 1974 Jul 1;140(1):87–104. doi: 10.1084/jem.140.1.87. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Glauert A. M., Thornley M. J. The topography of the bacterial cell wall. Annu Rev Microbiol. 1969;23:159–198. doi: 10.1146/annurev.mi.23.100169.001111. [DOI] [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. McCoy E. C., Doyle D., Burda K., Corbeil L. B., Winter A. J. Superficial antigens of Campylobacter (Vibrio) fetus: characterization of antiphagocytic component. Infect Immun. 1975 Mar;11(3):517–525. doi: 10.1128/iai.11.3.517-525.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. McCoy E. C., Doyle D., Wiltberger H., Burda K., Winter A. J. Flagellar ultrastructure and flagella-associated antigens of Campylobacter fetus. J Bacteriol. 1975 Apr;122(1):307–315. doi: 10.1128/jb.122.1.307-315.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Miura T., Mizushima S. Separation and properties of outer and cytoplasmic membranes in Escherichia coli. Biochim Biophys Acta. 1969;193(2):268–276. doi: 10.1016/0005-2736(69)90188-6. [DOI] [PubMed] [Google Scholar]
  12. O'NEILL G. J., TODD J. P. Extraction of nucleic acid-free lipopolysaccharides from gram-negative bacteria. Nature. 1961 Apr 22;190:344–345. doi: 10.1038/190344b0. [DOI] [PubMed] [Google Scholar]
  13. OSBORN M. J. STUDIES ON THE GRAM-NEGATIVE CELL WALL. I. EVIDENCE FOR THE ROLE OF 2-KETO- 3-DEOXYOCTONATE IN THE LIPOPOLYSACCHARIDE OF SALMONELLA TYPHIMURIUM. Proc Natl Acad Sci U S A. 1963 Sep;50:499–506. doi: 10.1073/pnas.50.3.499. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Osborn M. J., Gander J. E., Parisi E., Carson J. Mechanism of assembly of the outer membrane of Salmonella typhimurium. Isolation and characterization of cytoplasmic and outer membrane. J Biol Chem. 1972 Jun 25;247(12):3962–3972. [PubMed] [Google Scholar]
  15. Rosenbusch J. P. Characterization of the major envelope protein from Escherichia coli. Regular arrangement on the peptidoglycan and unusual dodecyl sulfate binding. J Biol Chem. 1974 Dec 25;249(24):8019–8029. [PubMed] [Google Scholar]
  16. Schnaitman C. A. Comparison of the envelope protein compositions of several gram-negative bacteria. J Bacteriol. 1970 Dec;104(3):1404–1405. doi: 10.1128/jb.104.3.1404-1405.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Schnaitman C. A. Effect of ethylenediaminetetraacetic acid, Triton X-100, and lysozyme on the morphology and chemical composition of isolate cell walls of Escherichia coli. J Bacteriol. 1971 Oct;108(1):553–563. doi: 10.1128/jb.108.1.553-563.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Schnaitman C. A. Examination of the protein composition of the cell envelope of Escherichia coli by polyacrylamide gel electrophoresis. J Bacteriol. 1970 Nov;104(2):882–889. doi: 10.1128/jb.104.2.882-889.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Schnaitman C. A. Outer membrane proteins of Escherichia coli. 3. Evidence that the major protein of Escherichia coli O111 outer membrane consists of four distinct polypeptide species. J Bacteriol. 1974 May;118(2):442–453. doi: 10.1128/jb.118.2.442-453.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Schnaitman C. A. Outer membrane proteins of Escherichia coli. I. Effect of preparative conditions on the migration of protein in polyacrylamide gels. Arch Biochem Biophys. 1973 Aug;157(2):541–552. doi: 10.1016/0003-9861(73)90673-5. [DOI] [PubMed] [Google Scholar]
  21. Schnaitman C. A. Protein composition of the cell wall and cytoplasmic membrane of Escherichia coli. J Bacteriol. 1970 Nov;104(2):890–901. doi: 10.1128/jb.104.2.890-901.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Schnaitman C., Smith D., de Salsas M. F. Temperate Bacteriophage Which Causes the Production of a New Major Outer Membrane Protein by Escherichia coli. J Virol. 1975 May;15(5):1121–1130. doi: 10.1128/jvi.15.5.1121-1130.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Schurig G. D., Hall C. E., Burda K., Corbeil L. B., Duncan J. R., Winter A. J. Persistent genital tract infection with Vibrio fetus intestinalis associated with serotypic alteration of the infecting strain. Am J Vet Res. 1973 Nov;34(11):1399–1403. [PubMed] [Google Scholar]
  24. WEIDEL W., FRANK H., MARTIN H. H. The rigid layer of the cell wall of Escherichia coli strain B. J Gen Microbiol. 1960 Feb;22:158–166. doi: 10.1099/00221287-22-1-158. [DOI] [PubMed] [Google Scholar]
  25. WEISSBACH A., HURWITZ J. The formation of 2-keto-3-deoxyheptonic acid in extracts of Escherichia coli B. I. Identification. J Biol Chem. 1959 Apr;234(4):705–709. [PubMed] [Google Scholar]
  26. Weber K., Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [PubMed] [Google Scholar]
  27. Winter A. J. An antigenic analysis of Vibrio fetus. 3. Chemical, biologic, and antigenic properties of the endotoxin. Am J Vet Res. 1966 May;27(118):653–658. [PubMed] [Google Scholar]
  28. Winter A. J., Katz W., Martin H. H. Murein (peptidoglycan) structure of Vibrio fetus. Comparison of a venereal and an intestinal strain. Biochim Biophys Acta. 1971 Jul 20;244(1):58–64. doi: 10.1016/0304-4165(71)90120-6. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES