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. 1976 Apr;13(4):1266–1272. doi: 10.1128/iai.13.4.1266-1272.1976

Antibody-mediated immobilization of Campylobacter fetus: inhibition by a somatic antigen.

E C McCoy, H A Wiltberger, A J Winter
PMCID: PMC420748  PMID: 819375

Abstract

Immobilization tests were conducted on a wild-type strain of Campylobacter fetus subsp. intestinalis and on a mutant lacking an antiphagocytic cell surface component. Highly effective immobilization of the mutant, both as single cells and clumps of cells, was produced with an antiserum containing antibodies specific for the flagellar hook and filament and for the O antigen. Damage to flagellar hooks after reaction with this antiserum was observed only with cells of the mutant. Single-cell immobilization of the mutant was also produced with an antiserum specific for a heat-stable somatic antigen which was distinct from the O antigen and was exposed on the cell surface only of the mutant. Minimal immobilization of the wild strain was brought about by either of these antisera. It was shown also that O antibodies had no effect on the motility of either the wild strain or mutant. These findings indicate that antibody-mediated immobilization may be brought about by effects on the flagellar hook or cell body, as well as on the flagellar filament. Furthermore, the protection from immobilization afforded the bacterium by the antiphagocytic surface structure suggests a dual function for this virulence factor in the infected animal.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berg H. C., Anderson R. A. Bacteria swim by rotating their flagellar filaments. Nature. 1973 Oct 19;245(5425):380–382. doi: 10.1038/245380a0. [DOI] [PubMed] [Google Scholar]
  2. DePamphilis M. L., Adler J. Purification of intact flagella from Escherichia coli and Bacillus subtilis. J Bacteriol. 1971 Jan;105(1):376–383. doi: 10.1128/jb.105.1.376-383.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. DiPierro J. M., Doetsch R. N. Enzymatic reversibility of flagellar immobilization. Can J Microbiol. 1968 Apr;14(4):487–489. doi: 10.1139/m68-081. [DOI] [PubMed] [Google Scholar]
  4. Duncan J. R., Wilkie B. N., Hiestand F., Winter A. J. The serum and secretory immunoglobulins of cattle: characterization and quantitation. J Immunol. 1972 Apr;108(4):965–976. [PubMed] [Google Scholar]
  5. Fields K. L. Comparison of the action of colicins E1 and K on Escherichia coli with the effects of abortive infection by virulent bacteriophages. J Bacteriol. 1969 Jan;97(1):78–82. doi: 10.1128/jb.97.1.78-82.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. GRUBB R., SWAHN B. Destruction of some agglutinins but not of others by two sulfhydryl compounds. Acta Pathol Microbiol Scand. 1958;43(3):305–309. doi: 10.1111/j.1699-0463.1958.tb04899.x. [DOI] [PubMed] [Google Scholar]
  7. Guentzel M. N., Berry L. J. Motility as a virulence factor for Vibrio cholerae. Infect Immun. 1975 May;11(5):890–897. doi: 10.1128/iai.11.5.890-897.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lederberg J. BACTERIAL PROTOPLASTS INDUCED BY PENICILLIN. Proc Natl Acad Sci U S A. 1956 Sep;42(9):574–577. doi: 10.1073/pnas.42.9.574. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. McCoy E. C., Doyle D., Burda K., Corbeil L. B., Winter A. J. Superficial antigens of Campylobacter (Vibrio) fetus: characterization of antiphagocytic component. Infect Immun. 1975 Mar;11(3):517–525. doi: 10.1128/iai.11.3.517-525.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. McCoy E. C., Doyle D., Wiltberger H., Burda K., Winter A. J. Flagellar ultrastructure and flagella-associated antigens of Campylobacter fetus. J Bacteriol. 1975 Apr;122(1):307–315. doi: 10.1128/jb.122.1.307-315.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Silverman M., Simon M. Flagellar rotation and the mechanism of bacterial motility. Nature. 1974 May 3;249(452):73–74. doi: 10.1038/249073a0. [DOI] [PubMed] [Google Scholar]
  12. Vaituzis Z., Doetsch R. N. Relationship between cell wall, cytoplasmic membrane, and bacterial motility. J Bacteriol. 1969 Oct;100(1):512–521. doi: 10.1128/jb.100.1.512-521.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. WEIBULL C. The isolation of protoplasts from Bacillus megaterium by controlled treatment with lysozyme. J Bacteriol. 1953 Dec;66(6):688–695. doi: 10.1128/jb.66.6.688-695.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Winter A. J. An antigenic analysis of Vibrio fetus. 3. Chemical, biologic, and antigenic properties of the endotoxin. Am J Vet Res. 1966 May;27(118):653–658. [PubMed] [Google Scholar]

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