Abstract
Hemagglutinating antibody responses of Lewis-Wistar and Sprague-Dawley rats to graded doses of type-specific pneumococcal polysaccharide were measured. Rats given a small dose (0.2 to 50 mug)of type 1 or 8 polysaccharide intraperitoneally developed type-specific hemagglutinating antibody. Rats given larger doses of polysaccharide (greater than or equal to200 mug) did not develop detectable hemagglutinating antibody, and they were unresponsive to a subsequence injection of a small (and normally antigenic) dose of polysaccharide. There was prolonged antigenemia in rats injected with a large dose of polysaccharide. There was prolonged antigenemia in rats injected with a large dose of polysaccharide, and the kinetics of antigen clearance in these animals resembled that reported for mice with polysaccharide immunological paralysis. These results indicate that a phenomenon resembling immunological paralysis with type-specific pneumococcal polysaccharides can be produced in rats.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Ammann A. J., Pelger R. J. Determination of antibody to pneumococcal polysaccharides with chromic chloride-treated human red blood cells and indirect hemagglutination. Appl Microbiol. 1972 Nov;24(5):679–683. doi: 10.1128/am.24.5.679-683.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brooke M. S. A hemagglutination test and a specific depolymerase for studying immunologic paralysis in mice. J Immunol. 1966 Feb;96(2):358–363. [PubMed] [Google Scholar]
- COHN M. The problem of specific inhibition of antibody synthesis in adult animals by immunization of embryos. Ann N Y Acad Sci. 1957 Mar 22;64(5):859–876. doi: 10.1111/j.1749-6632.1957.tb52480.x. [DOI] [PubMed] [Google Scholar]
- Coonrod J. D., Rytel M. W. Detection of type-specific pneumococcal antigens by counterimmunoelectrophoresis. I. Methodology and immunologic properties of pneumococcal antigens. J Lab Clin Med. 1973 May;81(5):770–777. [PubMed] [Google Scholar]
- FELTON L. D., KAUFFMANN G., PRESCOTT B., OTTINGER B. Studies on the mechanism of the immunological paralysis induced in mice by pneumococcal polysaccharides. J Immunol. 1955 Jan;74(1):17–26. [PubMed] [Google Scholar]
- Halliday W. J. Immunological paralysis of mice with pneumococcal polysaccharide antigens. Bacteriol Rev. 1971 Sep;35(3):267–289. doi: 10.1128/br.35.3.267-289.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Howard J. G., Christie G. H., Jacob M. J., Elson J. Studies on immunological paralysis. 3. Recirculation and antibody-neutralizing activity of 14C-labelled type 3 pneumococcal polysaccharide in paralysed mice. Clin Exp Immunol. 1970 Oct;7(4):583–596. [PMC free article] [PubMed] [Google Scholar]
- MORGAN P., WATSON D. W., CROMARTIE W. J. Immunization of rabbits with type II pneumococcal polysaccharide. Proc Soc Exp Biol Med. 1952 Jul;80(3):512–516. doi: 10.3181/00379727-80-19675. [DOI] [PubMed] [Google Scholar]
- SCHMIDT M. H., WOLFE H. R. Precipitin production in chickens. IX. A quantitative study of the antibody response to nine different purified substances. J Immunol. 1953 Oct;71(4):214–219. [PubMed] [Google Scholar]