Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1976 Jun;13(6):1626–1632. doi: 10.1128/iai.13.6.1626-1632.1976

Rapid quantitation of interferon with chronically oncornavirus-producing cells.

M Aboud, O Weiss, S Salzberg
PMCID: PMC420811  PMID: 61174

Abstract

The capacity of interferon to inhibit virus production in cells chronically infected with oncornavirus enabled us to develop a simple system for interferon quantitation that was independent of exogenous viral infection. The release of the virus to the culture medium was determined by its reverse transcriptase activity. The inhibitory effect of interferon in this system was linearly proportional to the log of its dilution over a range between 5 and 80% inhibiton. The sensitivity of the system was comparable to that of the vexicular stomatitis virus plaque reduction assay, whereas its reproducibility was found to be even better. This method is very rapid and can be completed within less than 24 h.

Full text

PDF
1626

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allen P. T., Giron D. J. Rapid sensitive assay for interferons based on the inhibition of MM virus nucleic acid synthesis. Appl Microbiol. 1970 Sep;20(3):317–322. doi: 10.1128/am.20.3.317-322.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Armstrong J. A. Semi-micro, dye-binding assay for rabbit interferon. Appl Microbiol. 1971 Apr;21(4):723–725. doi: 10.1128/am.21.4.723-725.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Billiau A., Edy V. G., De Clercq E., Heremans H., De Somer P. Influence of interferon on the synthesis of virus particles in oncornavirus carrier cell lines. III. Survey of effects on A-, B- and C-type oncornaviruses. Int J Cancer. 1975 Jun 15;15(6):947–953. doi: 10.1002/ijc.2910150610. [DOI] [PubMed] [Google Scholar]
  4. Billiau A., Edy V. G., Sobis H., de Somer P. Influence of interferon on virus-particle synthesis in oncornavirus-carrier lines. II. Evidence for a direct effect on particle release. Int J Cancer. 1974 Sep 15;14(3):335–340. doi: 10.1002/ijc.2910140306. [DOI] [PubMed] [Google Scholar]
  5. Billiau A., Sobis H., De Somer P. Influence of interferon on virus particle formation in different oncornavirus carrier cell lines. Int J Cancer. 1973 Nov 15;12(3):646–653. doi: 10.1002/ijc.2910120313. [DOI] [PubMed] [Google Scholar]
  6. Campbell J. B., Grunberger T., Kochman M. A., White S. L. A microplaque reduction assay for human and mouse interferon. Can J Microbiol. 1975 Aug;21(8):1247–1253. doi: 10.1139/m75-186. [DOI] [PubMed] [Google Scholar]
  7. FINTER N. B. QUANTITATIVE HAEMADSORPTION, A NEW ASSAY TECHNIQUE. I. ASSAY OF INTERFERON. Virology. 1964 Dec;24:589–597. doi: 10.1016/0042-6822(64)90212-0. [DOI] [PubMed] [Google Scholar]
  8. Falcoff E., Falcoff R., Lebleu B., Revel M. Correlation between the antiviral effect of interferon treatment and the inhibition of in vitro mRNA translation in noninfected L cells. J Virol. 1973 Sep;12(3):421–430. doi: 10.1128/jvi.12.3.421-430.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Friedman R. M., Chang E. H., Ramseur J. M., Myers M. W. Interferon-directed inhibition of chronic murine leukemia virus production in cell cultures: lack of effect on intracellular viral markers. J Virol. 1975 Sep;16(3):569–574. doi: 10.1128/jvi.16.3.569-574.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Friedman R. M., Ramseur J. M. Inhibition of murine leukemia virus production in chronically infected AKR cells: a novel effect of interferon. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3542–3544. doi: 10.1073/pnas.71.9.3542. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. GIFFORD G. E., MUSSETT M. V., HELLER E. COMPARATIVE STUDIES ON THE PRODUCTION AND ASSAY OF INTERFERON. J Gen Microbiol. 1964 Mar;34:475–481. doi: 10.1099/00221287-34-3-475. [DOI] [PubMed] [Google Scholar]
  12. HERMODSSON S., PHILIPSON L. A SENSITIVE METHOD FOR INTERFERON ASSAY. Proc Soc Exp Biol Med. 1963 Dec;114:574–579. doi: 10.3181/00379727-114-28735. [DOI] [PubMed] [Google Scholar]
  13. Knight E., Jr Heterogeneity of purified mouse interferons. J Biol Chem. 1975 Jun 10;250(11):4139–4144. [PubMed] [Google Scholar]
  14. LINDENMANN J., GIFFORD G. E. Studies on vaccinia virus plaque formation and its inhibition by interferon. III. A simplified plaque inhibition assay of interferon. Virology. 1963 Mar;19:302–309. doi: 10.1016/0042-6822(63)90068-0. [DOI] [PubMed] [Google Scholar]
  15. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  16. Lieberman D., Voloch Z., Aviv H., Nudel U., Revel M. Effects of interferon on hemoglobin synthesis and leukemia virus production in Friend cells. Mol Biol Rep. 1974 Dec;1(8):447–451. doi: 10.1007/BF00360670. [DOI] [PubMed] [Google Scholar]
  17. McAllister R. M., Nicolson M., Gardner M. B., Rongey R. W., Rasheed S., Sarma P. S., Huebner R. J., Hatanaka M., Oroszlan S., Gilden R. V. C-type virus released from cultured human rhabdomyosarcoma cells. Nat New Biol. 1972 Jan 5;235(53):3–6. doi: 10.1038/newbio235003a0. [DOI] [PubMed] [Google Scholar]
  18. McCormick J. J., Larson L. J., Rich M. A. RNase inhibition of reverse transcriptase activity in human milk. Nature. 1974 Oct 25;251(5477):737–740. doi: 10.1038/251737a0. [DOI] [PubMed] [Google Scholar]
  19. McWilliams M., Finkelstein M. S., Allen P. T., Giron D. J. Assay of chick interferons by the inhibition of viral ribonucleic acid synthesis. Appl Microbiol. 1971 May;21(5):959–961. doi: 10.1128/am.21.5.959-961.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Pidot A. L. Dye uptake assay: an efficient and sensitive method for human interferon titration. Appl Microbiol. 1971 Oct;22(4):671–677. doi: 10.1128/am.22.4.671-677.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Salzberg S., Robin M. S., Green M. Appearance of virus-specific RNA, virus particles, and cell surface changes in cells rapidly transformed by the murine sarcoma virus. Virology. 1973 May;53(1):186–195. doi: 10.1016/0042-6822(73)90477-7. [DOI] [PubMed] [Google Scholar]
  22. Sedmak J. J., Grossberg S. E. Interferon bioassay: reduction in yield of myxovirus neuraminidases. J Gen Virol. 1973 Oct;21:1–7. doi: 10.1099/0022-1317-21-1-1. [DOI] [PubMed] [Google Scholar]
  23. Sedmak J. J., Grossberg S. E., Jameson P. The neuraminidase yield-reduction bioassay of human other interferons. Proc Soc Exp Biol Med. 1975 Jun;149(2):433–438. doi: 10.3181/00379727-149-38822. [DOI] [PubMed] [Google Scholar]
  24. Stewart W. E., 2nd, De Clercq E., De Somer P. Cellular alteration by interferon: a virus-free system for assaying interferon. J Virol. 1972 Nov;10(5):896–901. doi: 10.1128/jvi.10.5.896-901.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Temin H. M., Baltimore D. RNA-directed DNA synthesis and RNA tumor viruses. Adv Virus Res. 1972;17:129–186. doi: 10.1016/s0065-3527(08)60749-6. [DOI] [PubMed] [Google Scholar]
  26. Tilles J. G., Finland M. Microassay for human and chick cell interferons. Appl Microbiol. 1968 Nov;16(11):1706–1707. doi: 10.1128/am.16.11.1706-1707.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES