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. 1976 Jul;14(1):266–270. doi: 10.1128/iai.14.1.266-270.1976

Nonpilar surface appendages of colony type 1 and colony type 4 gonococci.

Z A McGee, J Gross, R R Dourmashkin, D Taylor-Robinson
PMCID: PMC420872  PMID: 820643

Abstract

The nature and distribution of surface appendages morphologically distinct from pili were examined in three strains of gonococci. As reported previously by others, large appendages were seen emanating from colony type 1 gonococci when negatively stained preparations were examined by electron microscopy. Whereas the diameter of pili was 4 to 6 nm, the appendages varied from 8 to 40 nm in diameter with bulbous enlargements of up to 130 nm. However, in contrast to previous findings, the appendages were not limited to virulence-associated colonial types but occurred with almost equal frequency on organisms from colonial types 1 and 4. Thus, the presence of these appendages on gonococci was unrelated to colonial morphology, and there is currently no reason to associate them with gonococcal virulence. The large appendages were seldom seen if the organisms were suspended in isotonic fluid rather than in distilled water, which was used in previous studies. This suggests that the large appendages may occur infrequently in the isotonic environment of tissues or in slightly hypertonic purulent exudates. Nevertheless, we did observe small appendages or cell wall blebs on the surface of gonococci suspended in isotonic medium and noted similar structures as spheres free in the surrounding milieu. These findings suggest that the material of which the large appendages consist may form small appendages or cell wall blebs and be shed into the milieu under isotonic conditions. Such cell wall blebs have been demonstrated on meningococci and been shown to consist of endotoxin.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BAKER R. F. THE FINE STRUCTURE OF STROMALYTIC FORMS PRODUCED BY OSMOTIC HEMOLYSIS OF RED BLOOD CELLS. J Ultrastruct Res. 1964 Dec;11:494–507. doi: 10.1016/s0022-5320(64)80078-2. [DOI] [PubMed] [Google Scholar]
  2. Buchanan T. M., Gotschlich E. C. Studies on gonococcus infection. 3. Correlation of gonococcal colony morphology with infectivity for the chick embryo. J Exp Med. 1973 Jan 1;137(1):196–200. doi: 10.1084/jem.137.1.196. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Devoe I. W., Gilchrist J. E. Release of endotoxin in the form of cell wall blebs during in vitro growth of Neisseria meningitidis. J Exp Med. 1973 Nov 1;138(5):1156–1167. doi: 10.1084/jem.138.5.1156. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Duguid J. P., Anderson E. S., Campbell I. Fimbriae and adhesive properties in Salmonellae. J Pathol Bacteriol. 1966 Jul;92(1):107–138. doi: 10.1002/path.1700920113. [DOI] [PubMed] [Google Scholar]
  5. Gibbs D. L., Roberts R. B. The interaction in vitro between human polymorphonuclear leukocytes and Neisseria gonorrhoeae cultivated in the chick embryo. J Exp Med. 1975 Jan 1;141(1):155–171. doi: 10.1084/jem.141.1.155. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Glauert A. M., Thornley M. J. The topography of the bacterial cell wall. Annu Rev Microbiol. 1969;23:159–198. doi: 10.1146/annurev.mi.23.100169.001111. [DOI] [PubMed] [Google Scholar]
  7. Grimble A., Armitage L. R. Surface structures of the gonococcus. Br J Vener Dis. 1974 Oct;50(5):354–359. doi: 10.1136/sti.50.5.354. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jephcott A. E., Reyn A., Birch-Andersen A. Neisseria gonorrhoeae 3. Demonstration of presumed appendages to cells from different colony types. Acta Pathol Microbiol Scand B Microbiol Immunol. 1971;79(3):437–439. doi: 10.1111/j.1699-0463.1971.tb00086.x. [DOI] [PubMed] [Google Scholar]
  9. Johnston K. H., Gotschlich E. C. Isolation and characterization of the outer membrane of Neisseria gonorrhoeae. J Bacteriol. 1974 Jul;119(1):250–257. doi: 10.1128/jb.119.1.250-257.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. KELLOGG D. S., Jr, PEACOCK W. L., Jr, DEACON W. E., BROWN L., PIRKLE D. I. NEISSERIA GONORRHOEAE. I. VIRULENCE GENETICALLY LINKED TO CLONAL VARIATION. J Bacteriol. 1963 Jun;85:1274–1279. doi: 10.1128/jb.85.6.1274-1279.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kellogg D. S., Jr, Cohen I. R., Norins L. C., Schroeter A. L., Reising G. Neisseria gonorrhoeae. II. Colonial variation and pathogenicity during 35 months in vitro. J Bacteriol. 1968 Sep;96(3):596–605. doi: 10.1128/jb.96.3.596-605.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. McGee Z. A., Johnson A. P., Taylor-Robinson D. Human fallopian tubes in organ culture: preparation, maintenance, and quantitation of damage by pathogenic microorganisms. Infect Immun. 1976 Feb;13(2):608–618. doi: 10.1128/iai.13.2.608-618.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mergenhagen S. E., Bladen H. A., Hsu K. C. Electron microscopic localization of endotoxic lipopolysaccharide in gram-negative organisms. Ann N Y Acad Sci. 1966 Jun 30;133(2):279–291. doi: 10.1111/j.1749-6632.1966.tb52371.x. [DOI] [PubMed] [Google Scholar]
  14. Meynell E., Meynell G. G., Datta N. Phylogenetic relationships of drug-resistance factors and other transmissible bacterial plasmids. Bacteriol Rev. 1968 Mar;32(1):55–83. doi: 10.1128/br.32.1.55-83.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Swanson J., Kraus S. J., Gotschlich E. C. Studies on gonococcus infection. I. Pili and zones of adhesion: their relation to gonococcal growth patterns. J Exp Med. 1971 Oct 1;134(4):886–906. doi: 10.1084/jem.134.4.886. [DOI] [PMC free article] [PubMed] [Google Scholar]

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