Skip to main content
Infection and Immunity logoLink to Infection and Immunity
. 1976 Aug;14(2):363–367. doi: 10.1128/iai.14.2.363-367.1976

Cell-mediated immunity to Vibrio cholerae with ribonucleic acid-protein fractions of V. cholerae L-form lysates.

S C Agarwal, T Sundararaj
PMCID: PMC420891  PMID: 971951

Abstract

Different L-form lysate vaccines of Vibrio cholerae serotypes Ogawa and Inaba and their combination along with ethyl alcohol-precipitated ribonucleic acid (E-RNA) and phenol-extracted RNA (P-RNA) fractions of V. cholerae Ogawa lysates were tested for production of cell-mediated immunity. Both E-RNA and P-RNA fractions induced an increase in leukocyte migration inhibition, macrophage migration inhibition, and macrophage aggregation. They also induced delayed hypersensitivity in rabbits. More consistent results were obtained with the P-RNA fraction.

Full text

PDF
363

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Agarwal S. C., Ganguly N. K. Experimental oral immunization with L-forms of Vibrio cholerae. Infect Immun. 1972 Jan;5(1):31–34. doi: 10.1128/iai.5.1.31-34.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Agarwal S. C., Ganguly N. K. Oral immunization with L-forms of Vibrio cholerae in human volunteers. Infect Immun. 1972 Jul;6(1):17–20. doi: 10.1128/iai.6.1.17-20.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Araujo F. G., Remington J. S. Protection against Toxoplasma gondii in mice immunized with Toxoplasma cell fractions, RNA and synthetic polyribonucleotides. Immunology. 1974 Oct;27(4):711–721. [PMC free article] [PubMed] [Google Scholar]
  4. Blanden R. V., Lefford M. J., Mackaness G. B. The host response to Calmette-Guérin bacillus infection in mice. J Exp Med. 1969 May 1;129(5):1079–1107. doi: 10.1084/jem.129.5.1079. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Collins F. M., Mackaness G. B. The relationship of delayed hypersensitivity to acquired antituberculous immunity. II. Effect of adjuvant on the allergenicity and immunogenicity of heat-killed tubercle bacilli. Cell Immunol. 1970 Sep;1(3):266–275. doi: 10.1016/0008-8749(70)90048-1. [DOI] [PubMed] [Google Scholar]
  6. DAVID J. R., AL-ASKARI S., LAWRENCE H. S., THOMAS L. DELAYED HYPERSENSITIVITY IN VITRO. I. THE SPECIFICITY OF INHIBITION OF CELL MIGRATION BY ANTIGENS. J Immunol. 1964 Aug;93:264–273. [PubMed] [Google Scholar]
  7. KIRBY K. S. A new method for the isolation of ribonucleic acids from mammalian tissues. Biochem J. 1956 Nov;64(3):405–408. doi: 10.1042/bj0640405. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. Lolekha S., Dray S., Gotoff S. P. Macrophage aggregation in vitro: a correlate of delayed hypersensitivity. J Immunol. 1970 Feb;104(2):296–304. [PubMed] [Google Scholar]
  10. Maini R. N., Roffe L. M., Magrath I. T., Dumonde D. C. Standardization of the leucocyte migration test. Int Arch Allergy Appl Immunol. 1973;45(1):308–321. doi: 10.1159/000231048. [DOI] [PubMed] [Google Scholar]
  11. Neiburger R. G., Youmans G. P., Youmans A. S. Relationship between tuberculin hypersensitivity and cellular immunity to infection in mice vaccinated with viable attenuated Mycobacterial cells or with Mycobacterial ribonucleic acid preparations. Infect Immun. 1973 Jul;8(1):42–47. doi: 10.1128/iai.8.1.42-47.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Simon H. B., Sheagren J. N. Cellular immunity in vitro. I. Immunologically mediated enhancement of macrophage bactericidal capacity. J Exp Med. 1971 Jun 1;133(6):1377–1389. doi: 10.1084/jem.133.6.1377. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Smith R. A., Bigley N. J. Ribonucleic acid-protein fractions of virulent Salmonella typhimurium as protective immunogens. Infect Immun. 1972 Sep;6(3):377–383. doi: 10.1128/iai.6.3.377-383.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Smith R. L., Wysocki J. A., Bruun J. N., De Courcy S. J., Jr, Blakemore W. S., Mudd S. Efficacy of ribosomal preparations from Pseudomonas aeruginosa to protect against intravenous Pseudomonas challenge in mice. J Reticuloendothel Soc. 1974 Jan;15(1):22–30. [PubMed] [Google Scholar]
  15. Thompson H. C., Snyder I. S. Protection against pneumococcal infection by a ribosomal preparation. Infect Immun. 1971 Jan;3(1):16–23. doi: 10.1128/iai.3.1.16-23.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Venneman M. R., Bigley N. J., Berry L. J. Immunogenicity of Ribonucleic Acid Preparations Obtained from Salmonella typhimurium. Infect Immun. 1970 Jun;1(6):574–582. doi: 10.1128/iai.1.6.574-582.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Winston S., Berry L. J. Immunity induced by ribosomal extracts from Staphylococcus aureus. J Reticuloendothel Soc. 1970 Jul;8(1):66–73. [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES