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. 1976 Aug;14(2):372–375. doi: 10.1128/iai.14.2.372-375.1976

Cell-mediated immune response to products of Actinomyces viscosus cultures.

D W Turner, B S Roberson, R W Longton
PMCID: PMC420893  PMID: 971952

Abstract

Hartley strain guinea pigs were sensitized with 0.5 ml of concentrated cell-free Actinomyces viscosus culture supernatant fluids mixed with Freund complete adjuvant. Fourteen to 16 days later the animals were challenged by intradermal injection with 0.1 ml of the culture supernatant, and the reactions were observed at 4, 8, 16, 24, and 48 h. Peritoneal exudate cells from sensitized animals were used for determination of migration inhibition factor, and guinea pig peripheral blood served as a source of cells for determining the induction of mitogenesis by antigenic material. Skin responses were consistently positive to challenge with the test material, whereas reactions to noninoculated culture medium were negative. Sensitized cells, challenged with antigen, resulted in 60% or greater inhibition of migration of indicator cells in migration inhibition factor experiments. Tests for mitogenesis showed a greater than fourfold increase in isotope uptake when sensitized cells were challenged with test material. The data are consistent with the suggestion that A. viscosus culture supernatants contain substances that induce cell-mediated immune responses in guinea pigs.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Freedman H. L., Listgarten M. A., Taichman N. S. Electron microscopic features of chronically inflamed human gingiva. J Periodontal Res. 1968;3(4):313–327. doi: 10.1111/j.1600-0765.1968.tb01937.x. [DOI] [PubMed] [Google Scholar]
  2. Gerencser M. A., Slack J. M. Identification of human strains of Actinomyces viscosus. Appl Microbiol. 1969 Jul;18(1):80–87. doi: 10.1128/am.18.1.80-87.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Horton J. E., Leikin S., Oppenheim J. J. Human lymphoproliferative reaction to saliva and dental plaque-deposits: an in vitro correlation with periodontal disease. J Periodontol. 1972 Sep;43(9):522–527. doi: 10.1902/jop.1972.43.9.522. [DOI] [PubMed] [Google Scholar]
  4. Horton J. E., Raisz L. G., Simmons H. A., Oppenheim J. J., Mergenhagen S. E. Bone resorbing activity in supernatant fluid from cultured human peripheral blood leukocytes. Science. 1972 Sep 1;177(4051):793–795. doi: 10.1126/science.177.4051.793. [DOI] [PubMed] [Google Scholar]
  5. Ivanyi L., Lehner T. Stimulation of lymphocyte transformation by bacterial antigens in patients with periodontal disease. Arch Oral Biol. 1970 Nov;15(11):1089–1096. doi: 10.1016/0003-9969(70)90121-4. [DOI] [PubMed] [Google Scholar]
  6. Ivanyi L., Wilton J. M., Lehner T. Cell-mediated immunity in periodontal disease; cytotoxicity, migration inhibition and lymphocyte transformation studies. Immunology. 1972 Jan;22(1):141–145. [PMC free article] [PubMed] [Google Scholar]
  7. JORDAN H. V., KEYES P. H. AEROBIC, GRAM-POSITIVE, FILAMENTOUS BACTERIA AS ETIOLOGIC AGENTS OF EXPERIMENTAL PERIODONTAL DISEASE IN HAMSTERS. Arch Oral Biol. 1964 Jul-Aug;9:401–414. doi: 10.1016/0003-9969(64)90025-1. [DOI] [PubMed] [Google Scholar]
  8. KEYES P. H., JORDAN H. V. PERIODONTAL LESIONS IN THE SYRIAN HAMSTER. III. FINDINGS RELATED TO AN INFECTIOUS AND TRANSMISSIBLE COMPONENT. Arch Oral Biol. 1964 Jul-Aug;9:377–400. doi: 10.1016/0003-9969(64)90024-x. [DOI] [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. McCabe R. M., Hageage G. J., Jr, Keyes P. H. A simple method for estimating viable filamentous bacteria in plaque. J Dent Res. 1975 Jan-Feb;54(1):195–195. doi: 10.1177/00220345750540012801. [DOI] [PubMed] [Google Scholar]
  11. Nisengard R. J., Beutner E. H. Relation of immediate hypersensitivity to periodontitis in animals and man. J Periodontol. 1970 Apr;41(4):223–227. doi: 10.1902/jop.1970.41.4.223. [DOI] [PubMed] [Google Scholar]
  12. Okada H., Yoshikawa Y., Sano S., Inui M., Nakata H., Fukuda T., Aono M. Induction of cellular hypersensitivity to oral microorganisms in guinea-pigs. Arch Oral Biol. 1975 Apr;20(4):231–233. doi: 10.1016/0003-9969(75)90116-8. [DOI] [PubMed] [Google Scholar]
  13. Socransky S. S. Relationship of bacteria to the etiology of periodontal disease. J Dent Res. 1970 Mar-Apr;49(2):203–222. doi: 10.1177/00220345700490020401. [DOI] [PubMed] [Google Scholar]
  14. Sussman H. I., Bartels H. A., Stahl S. S. The potential of microorganisms to invade the lamina propria of human gingival tissues. J Periodontol. 1969 Apr;40(4):210–215. doi: 10.1902/jop.1969.40.4.210. [DOI] [PubMed] [Google Scholar]
  15. Wolstencroft R. A., Dumonde D. C. In vitro studies of cell-mediated immunity. I. Induction of lymphocyte transformation by a soluble "mitogenic" factor derived from interaction of sensitized guinea-pig lymphoid cells with specific antigen. Immunology. 1970 Apr;18(4):599–610. [PMC free article] [PubMed] [Google Scholar]

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