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. 1976 Sep;14(3):631–635. doi: 10.1128/iai.14.3.631-635.1976

Hepatitis B antigen-associated deoxyribonucleic acid polymerase activity and e antigen/anti-e system.

M Imai, F C Tachibana, Y Moritsugu, Y Miyakawa, M Mayumi
PMCID: PMC420933  PMID: 965088

Abstract

Serum samples of 403 asymptomatic blood donors carrying hepatitis B surface antigen (HB5Ag) were concentrated threefold and tested for e antigen and antibody to e antigen (anti-e) by immunodiffusion. Hepatitis B antigen (HBAg)-associated deoxyribonucleic acid (DNA) polymerase activity was specifically determined by the difference in incorporation of [methyl-3H]thymidine 5' -triphosphate into DNA by an aliquot of centrifuged serum samples after it had been treated either with normal rabbit serum or with rabbit antibody to HBSAg. All of 58 serum samples containing e antigen revealed HBAg-associated DNA polymerase activity, whereas none of 96 samples containing anti-e did. In the remaining 249 samples in which neither e antigen nor anti-e was found, 62 showed specific DNA polymerase activity, although at lower levels than the samples containing e antigen.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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