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. 1977 May;16(2):461–466. doi: 10.1128/iai.16.2.461-466.1977

Characterization of antigens from type A and B yeast cells of Histoplasma capsulatum.

W Y Mok, H R Buckley, C C Campbell
PMCID: PMC420973  PMID: 193788

Abstract

The antigenic composition of cytoplasmic extract and culture filtrate antigens of type A and B yeast cells of Histoplasma capsulatum grown in a synthetic medium was studied. These preparations from type A and B yeast cells contained varying amounts of protein, carbohydrates, and protein-carbohydrate complexes. The antigenic analysis of these preparations was performed by antigen-antiserum absorption with subsequent immunodiffusion and cross-immunoelectrophoresis with absorption in situ in an intermediate gel. All protein antigens observed in culture filtrate of either type A or B yeast cells were also present in the cytoplasmic extracts of the same type. The cytoplasmic extract of type A and B yeast cells each contained certain characteristic antigens that were not shared by the other type. Rocket immunoelectrophoresis with different molecular weight fractions of the antigenic preparations from both types indicated the polydisperse nature of Histoplasma yeast cell antigens.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Axelsen N. H., Bock E. Identification and quantitation of antigens and antibodies by means of quantitative immunoelectrophoresis. A survey of methods. J Immunol Methods. 1972 Jan;1(2):109–121. doi: 10.1016/0022-1759(72)90038-5. [DOI] [PubMed] [Google Scholar]
  2. Axelsen N. H. Quantitative immunoelectrophoretic methods as tools for a polyvalent approach to standardization in the immunochemistry of Candida albicans. Infect Immun. 1973 Jun;7(6):949–960. doi: 10.1128/iai.7.6.949-960.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Berliner M. D. Primary subcultures of Histoplasma capsulatum. I. Macro and micro-morphology of the mycelial phase. Sabouraudia. 1968 Feb;6(2):111–118. [PubMed] [Google Scholar]
  4. CAMPBELL C. C. Antigenic fractions of Histoplasma capsulatum. Am J Public Health Nations Health. 1953 Jun;43(6 Pt 1):712–717. doi: 10.2105/ajph.43.6_pt_1.712. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Campbell C. C., Berliner M. D. Virulence differences in mice of type A and B Histoplasma capsulatum yeasts grown in continuous light and total darkness. Infect Immun. 1973 Oct;8(4):677–678. doi: 10.1128/iai.8.4.677-678.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Chick E. W., Baum G. L., Furcolow M. L., Huppert M., Kaufman L., Pappagianis R. Scientific Assembly statement. The use of skin tests and serologic tests in histoplasmosis, coccidioidomycosis, and blastomycosis, 1973. Am Rev Respir Dis. 1973 Jul;108(1):156–159. doi: 10.1164/arrd.1973.108.1.156. [DOI] [PubMed] [Google Scholar]
  7. Daniels L. S., Berliner M. D., Campbell C. C. Varying virulence in rabbits infected with different filamentous types of Histoplasma capsulatum. J Bacteriol. 1968 Nov;96(5):1535–1539. doi: 10.1128/jb.96.5.1535-1539.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Evans R. J., Bauer D. H., Bandemer S. L., Vaghefi S. B., Flegal C. J. Structure of egg yolk very low density lipoprotein. Polydispersity of the very low density lipoprotein and the role of lipovitellenin in the structure. Arch Biochem Biophys. 1973 Feb;154(2):493–500. doi: 10.1016/0003-9861(73)90001-5. [DOI] [PubMed] [Google Scholar]
  9. HILL G. B., CAMPBELL C. C. A further evaluation of histoplasmin and yeast phase antigen of Histoplasma capsulatum in the complement fixation test. J Lab Clin Med. 1956 Aug;48(2):255–263. [PubMed] [Google Scholar]
  10. Henneberry R. C., Carlton B. C. Characterization of the polydisperse closed circular deoxyribonucleic acid molecules of Bacillus megaterium. J Bacteriol. 1973 May;114(2):625–631. doi: 10.1128/jb.114.2.625-631.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  12. Lee K. L., Rega M. E., Watson R. R., Campbell C. C. Identification of yeast phase of pathogenic fungi by the specificity of their aminopeptidase(s). Sabouraudia. 1975 Jul;13(2):132–141. doi: 10.1080/00362177585190251. [DOI] [PubMed] [Google Scholar]
  13. Levin S. The fungal skin test as a diagnostic hindrance. J Infect Dis. 1970 Oct;122(4):343–345. doi: 10.1093/infdis/122.4.343. [DOI] [PubMed] [Google Scholar]
  14. MARKOWITZ H. POLYSACCHARIDE ANTIGENS FROM HISTOPLASMA CAPSULATUM. Proc Soc Exp Biol Med. 1964 Mar;115:697–700. doi: 10.3181/00379727-115-29010. [DOI] [PubMed] [Google Scholar]
  15. Murray I. G., Buckley H. R., Turner G. C. Serological evidence of Candida infection after open-heart surgery. J Med Microbiol. 1969 Nov 4;2(4):463–469. doi: 10.1099/00222615-2-4-463. [DOI] [PubMed] [Google Scholar]
  16. SALVIN S. B., SMITH R. F. Antigens from the yeast phase of Histoplasma capsulatum. III. Isolation, properties, and activity of a protein-carbohydrate complex. J Infect Dis. 1959 Jul-Aug;105(1):45–53. doi: 10.1093/infdis/105.1.45. [DOI] [PubMed] [Google Scholar]
  17. Sweet G. H. Antigens of Histoplasma capsulatum. II. Separation and characterization of yeast-phase precipitinogens. Am Rev Respir Dis. 1971 Sep;104(3):401–407. doi: 10.1164/arrd.1971.104.3.401. [DOI] [PubMed] [Google Scholar]
  18. Syverson R. E., Buckley H. R., Campbell C. C. Cytoplasmic antigens unique to the mycelial or yeast phase of Candida albicans. Infect Immun. 1975 Nov;12(5):1184–1188. doi: 10.1128/iai.12.5.1184-1188.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Tewari R. P., Berkhout F. J. Comparative pathogenicity of albino and brown types of Histoplasma capsulatum for mice. J Infect Dis. 1972 May;125(5):504–508. doi: 10.1093/infdis/125.5.504. [DOI] [PubMed] [Google Scholar]
  20. White T. G., Garrison R. G., Clark J. L., Johns L. E., Jr Antigens of Histoplasma capsulatum. I. Serologic differences between histoplasmins derived from albino and brown filamentous isolates. J Infect Dis. 1972 Mar;125(3):276–282. doi: 10.1093/infdis/125.3.276. [DOI] [PubMed] [Google Scholar]

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