Abstract
Sheep erythrocytes sensitized with the heat-labile enterotoxin (LT) of Escherichia coli exhibited passive immune hemolysis (PIH) when exposed to specific antitoxin and complement. Thus, PIH serves as the basis for an in vitro serological assay for LT that is sufficiently specific and sensitive to differentiate LT-positive and LT-negative E. coli isolates. The PIH assay for E. coli LT has been performed with the standard Microtiter system and also by a tube method employing the spectrophotometric determination of hemoglobin release. The spectrophotometric method enhances the sensitivity, accuracy, and objectivity of the PIH assay. The increased sensitivity of the spectrophotometric method also facilitates the identification of LT-positive cultures employing polymyxin "mini-extracts" of whole overnight (18 h) broth cultures of 2.0% Casamino Acid-0.6% yeast extract-salts medium rather than mini-extracts of cells derived from 3.5-h subcultures. Thus, large numbers of E. coli isolates can be individually tested for LT in less than 24 h after broth inoculation by a rapid in vitro assay which requires anti-LT serum as the only specific reagent.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Donta S. T., Smith D. M. Stimulation of steroidogenesis in tissue culture by enterotoxigenic Escherichia coli and its neutralization by specific antiserum. Infect Immun. 1974 Mar;9(3):500–505. doi: 10.1128/iai.9.3.500-505.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DuPont H. L., Olarte J., Evans D. G., Pickering L. K., Galindo E., Evans D. J. Comparative susceptibility of latin american and united states students to enteric pathogens. N Engl J Med. 1976 Dec 30;295(27):1520–1521. doi: 10.1056/NEJM197612302952707. [DOI] [PubMed] [Google Scholar]
- Evans D. G., Evans D. J., Jr, Gorbach S. L. Identification of enterotoxigenic Escherichia coli and serum antitoxin activity by the vascular permeability factor assay. Infect Immun. 1973 Nov;8(5):731–735. doi: 10.1128/iai.8.5.731-735.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Evans D. J., Evans D. G., Gorbach S. L. Polymyxin B-Induced Release of Low-Molecular-Weight, Heat-Labile Enterotoxin from Escherichia coli. Infect Immun. 1974 Nov;10(5):1010–1017. doi: 10.1128/iai.10.5.1010-1017.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Evans D. J., Jr, Evans D. G., Gorbach S. L. Production of vascular permeability factor by enterotoxigenic Escherichia coli isolated from man. Infect Immun. 1973 Nov;8(5):725–730. doi: 10.1128/iai.8.5.725-730.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Evans D. J., Jr, Evans D. G. Inhibition of immune hemolysis: serological assay for the heat-labile enterotoxin of Excherichia coli. J Clin Microbiol. 1977 Jan;5(1):100–105. doi: 10.1128/jcm.5.1.100-105.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Evans D. J., Jr, Evans D. G. Three characteristics associated with enterotoxigenic Escherichia coli isolated from man. Infect Immun. 1973 Sep;8(3):322–328. doi: 10.1128/iai.8.3.322-328.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Giannella R. A. Suckling mouse model for detection of heat-stable Escherichia coli enterotoxin: characteristics of the model. Infect Immun. 1976 Jul;14(1):95–99. doi: 10.1128/iai.14.1.95-99.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gorbach S. L., Kean B. H., Evans D. G., Evans D. J., Jr, Bessudo D. Travelers' diarrhea and toxigenic Escherichia coli. N Engl J Med. 1975 May 1;292(18):933–936. doi: 10.1056/NEJM197505012921801. [DOI] [PubMed] [Google Scholar]
- Guerrant R. L., Moore R. A., Kirschenfeld P. M., Sande M. A. Role of toxigenic and invasive bacteria in acute diarrhea of childhood. N Engl J Med. 1975 Sep 18;293(12):567–572. doi: 10.1056/NEJM197509182931201. [DOI] [PubMed] [Google Scholar]
- Gyles C. L. Immunological study of the heat-labile enterotoxins of Escherichia coli and Vibrio cholerae. Infect Immun. 1974 Mar;9(3):564–570. doi: 10.1128/iai.9.3.564-570.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gyles C. L. Relationships among heat-labile enterotoxins of Escherichia coli and Vibrio cholerae. J Infect Dis. 1974 Mar;129(3):277–283. doi: 10.1093/infdis/129.3.277. [DOI] [PubMed] [Google Scholar]
- Holmgren J., Söderlind O., Wadström T. Cross-reactivity between heat labile enterotoxins of Vibrio cholerae and Escherichia coli in neutralization tests in rabbit ileum and skin. Acta Pathol Microbiol Scand B Microbiol Immunol. 1973 Dec;81(6):757–762. doi: 10.1111/j.1699-0463.1973.tb02272.x. [DOI] [PubMed] [Google Scholar]
- Klipstein F. A., Holdeman L. V., Corcino J. J., Moore W. E. Enterotoxigenic intestinal bacteria in tropical sprue. Ann Intern Med. 1973 Nov;79(5):632–641. doi: 10.7326/0003-4819-79-5-632. [DOI] [PubMed] [Google Scholar]
- Klipstein F. A., Short H. B., Engert R. F., Jean L., Weaver G. A. Contamination of the small intestine by enterotoxigenic coliform bacteria among the rural population of Haiti. Gastroenterology. 1976 Jun;70(6):1035–1041. [PubMed] [Google Scholar]
- Merson M. H., Morris G. K., Sack D. A., Wells J. G., Feeley J. C., Sack R. B., Creech W. B., Kapikian A. Z., Gangarosa E. J. Travelers' diarrhea in Mexico. A prospective study of physicians and family members attending a congress. N Engl J Med. 1976 Jun 10;294(24):1299–1305. doi: 10.1056/NEJM197606102942401. [DOI] [PubMed] [Google Scholar]
- Mundell D. H., Anselmo C. R., Wishnow R. M. Factors influencing heat-labile Escherichia coli enterotoxin activity. Infect Immun. 1976 Aug;14(2):383–388. doi: 10.1128/iai.14.2.383-388.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nalin D. R., al-Mahmud A., Curlin G., Ahmed A., Peterson J. Cholera toxoid boosts serum Escherichia coli antitoxin in humans. Infect Immun. 1974 Oct;10(4):747–749. doi: 10.1128/iai.10.4.747-749.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sack R. B., Hirschborn N., Woodward W. E., Sack D. A., Cash R. A. Antibodies to heat-labile Escherichia coli enterotoxin in Apaches in Whiteriver, Arizona. Infect Immun. 1975 Dec;12(6):1475–1477. doi: 10.1128/iai.12.6.1475-1477.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sack R. B. Human diarrheal disease caused by enterotoxigenic Escherichia coli. Annu Rev Microbiol. 1975;29:333–353. doi: 10.1146/annurev.mi.29.100175.002001. [DOI] [PubMed] [Google Scholar]
- Sack R. B., Jacobs B., Mitra R. Antitoxin responses to infections with enterotoxigenic Escherichia coli. J Infect Dis. 1974 Mar;129(3):330–335. doi: 10.1093/infdis/129.3.330. [DOI] [PubMed] [Google Scholar]
- Smith N. W., Sack R. B. Immunologic cross-reactions of enterotoxins from Escherichia coli and Vibrio cholerae. J Infect Dis. 1973 Feb;127(2):164–170. doi: 10.1093/infdis/127.2.164. [DOI] [PubMed] [Google Scholar]