Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1977 May;16(2):623–627. doi: 10.1128/iai.16.2.623-627.1977

Homogeneity of protein serotype antigens in Neisseria meningitidis group A.

J E Sippel, A Quan
PMCID: PMC421002  PMID: 405327

Abstract

Serotype antigens (STA), which have been shown to be constituents of outer-membrane protein, were extracted from group A meningococci with 0.2 M LiCl and pelleted by centrifugation at 150,000 X g. The STAs from 100 group A strains, which had been isolated from cases and carriers in various geographical locations, were compared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Every STA produced three major protein bands with molecular weights of approximately 35,000, 39,000, and 45,000, respectively. This SDS-PAGE pattern is clearly distinct from that produced by the serotype of group B and C meningococci most commonly isolated from cases (group B type 2 and group C type 2). The group A STAs were also indistinguishable by immunodiffusion. However, differences in bactericidal reactions were demonstrated, suggesting that there are other antigens that play a role in antibody response.

Full text

PDF
623

Images in this article

625Image
on p.625
625Image
on p.625

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Altmann G., Egoz N., Bogokovsky B. Observations on asymptomatic infections with Neisseria meningitidis. Am J Epidemiol. 1973 Dec;98(6):446–452. doi: 10.1093/oxfordjournals.aje.a121574. [DOI] [PubMed] [Google Scholar]
  2. Apicella M. A. Immunological and biochemical studies of meningococcal C polysaccharides isolated by diethylaminoethyl chromatography. Infect Immun. 1976 Jul;14(1):106–113. doi: 10.1128/iai.14.1.106-113.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Artenstein M. S., Gold R., Zimmerly J. G., Wyle F. A., Schneider H., Harkins C. Prevention of meningococcal disease by group C polysaccharide vaccine. N Engl J Med. 1970 Feb 19;282(8):417–420. doi: 10.1056/NEJM197002192820803. [DOI] [PubMed] [Google Scholar]
  4. Counts G. W., Seeley L., Beaty H. N. Identification of an epidemic strain of Neisseria meningitidis by bacteriocin typing. J Infect Dis. 1971 Jul;124(1):26–32. doi: 10.1093/infdis/124.1.26. [DOI] [PubMed] [Google Scholar]
  5. Farrell D. G., Dahl E. V. Nasopharyngeal carriers of Neisseria meningitidis. Studies among air force recruits. JAMA. 1966 Dec 12;198(11):1189–1192. [PubMed] [Google Scholar]
  6. Frasch C. E., Chapman S. S. Classification of Neisseria meningitidis group B into distinct serotypes. 3. Application of a new bactericidal-inhibition technique to distribution of serotypes among cases and carriers. J Infect Dis. 1973 Feb;127(2):149–154. doi: 10.1093/infdis/127.2.149. [DOI] [PubMed] [Google Scholar]
  7. Frasch C. E., Chapman S. S. Classification of Neisseria meningitidis group B into distinct serotypes. I. Serological typing by a microbactericidal method. Infect Immun. 1972 Jan;5(1):98–102. doi: 10.1128/iai.5.1.98-102.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Frasch C. E., Chapman S. S. Classification of Neisseria meningitidis group B into distinct serotypes. II. Extraction of type-specific antigens for serotyping by precipitin techniques. Infect Immun. 1972 Aug;6(2):127–133. doi: 10.1128/iai.6.2.127-133.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Frasch C. E., Chapman S. S. Classification of Neisseria meningitidis group B into distinct serotypes. IV. Preliminary chemical studies on the nature of the serotype antigen. Infect Immun. 1972 Nov;6(5):674–681. doi: 10.1128/iai.6.5.674-681.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Frasch C. E., Gotschlich E. C. An outer membrane protein of Neisseria meningitidis group B responsible for serotype specificity. J Exp Med. 1974 Jul 1;140(1):87–104. doi: 10.1084/jem.140.1.87. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Frasch C. E., McNelis R. M., Gotschlich E. C. Strain-specific variation in the protein and lipopolysaccharide composition of the group B meningococcal outer membrane. J Bacteriol. 1976 Aug;127(2):973–981. doi: 10.1128/jb.127.2.973-981.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Fraser P. K., Bailey G. K., Abbott J. D., Gill J. B., Walker D. J. The meningococcal carrier-rate. Lancet. 1973 Jun 2;1(7814):1235–1237. doi: 10.1016/s0140-6736(73)90541-2. [DOI] [PubMed] [Google Scholar]
  13. Gold R., Winklehake J. L., Mars R. S., Artenstein M. S. Identification of an epidemic strain of group C Neisseria meningitidis by bactericidal serotyping. J Infect Dis. 1971 Dec;124(6):593–597. doi: 10.1093/infdis/124.6.593. [DOI] [PubMed] [Google Scholar]
  14. Gold R., Wyle F. A. New Classification of Neisseria meningitidis by Means of Bactericidal Reactions. Infect Immun. 1970 May;1(5):479–484. doi: 10.1128/iai.1.5.479-484.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Greenfield S., Sheehe P. R., Feldman H. A. Meningococcal carriage in a population of "normal" families. J Infect Dis. 1971 Jan;123(1):67–73. doi: 10.1093/infdis/123.1.67. [DOI] [PubMed] [Google Scholar]
  16. Hassan A., Girgis N., Sippel J., Sorensen K., Yassin M. Use of sulfadiazine in the prophylaxis of group A meningococcal carriers in Egypt. Trop Geogr Med. 1974 Mar;26(1):87–90. [PubMed] [Google Scholar]
  17. Hill J. C., Weiss E. Protein fraction with immunogenic potential and low toxicity isolated from the cell wall of Neisseria meningitidis group B. Infect Immun. 1974 Sep;10(3):605–615. doi: 10.1128/iai.10.3.605-615.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Johnston K. H., Holmes K. K., Gotschlich E. C. The serological classification of Neisseria gonorrhoeae. I. Isolation of the outer membrane complex responsible for serotypic specificity. J Exp Med. 1976 Apr 1;143(4):741–758. doi: 10.1084/jem.143.4.741. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Kasper D. L., Seiler M. W. Immunochemical characterization of the outer membrane complex of Bacteroides fragilis subspecies fragilis. J Infect Dis. 1975 Oct;132(4):440–450. doi: 10.1093/infdis/132.4.440. [DOI] [PubMed] [Google Scholar]
  20. Kasper D. L., Winkelhake J. L., Brandt B. L., Artenstein M. S. Antigenic specificity of bactericidal antibodies in antisera to Neisseria meningitidis. J Infect Dis. 1973 Apr;127(4):378–387. doi: 10.1093/infdis/127.4.378. [DOI] [PubMed] [Google Scholar]
  21. Kingsbury D. T. Bacteriocin production by strains of Neisseria meningitidis. J Bacteriol. 1966 May;91(5):1696–1699. doi: 10.1128/jb.91.5.1696-1699.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Sanborn W. R., Bencić Z., Cvjetanović B., Gotschlich E. C., Pollock T. M., Sippel J. E. Trial of a serogroup A meningococcus polysaccharide vaccine in Nigeria. Prog Immunobiol Stand. 1971;5:497–505. [PubMed] [Google Scholar]
  23. Sippel J. E., Girgis N. I., el-Ghoroury A., Wahdan M. H., Hablas R., Rahka A. E. Prevalence of Neisseria meningitidis serogroups in Egypt. Trop Geogr Med. 1973 Dec;25(4):350–354. [PubMed] [Google Scholar]
  24. Wahdan M. H., Rizk F., el-Akkad A. M., el-Ghoroury A. A., Hablas R., Girgis N. I., Amer A., Boctar W., Sippel J. E., Gotschlich E. C. A controlled field trial of a serogroup A meningococcal polysaccharide vaccine. Bull World Health Organ. 1973 Jun;48(6):667–673. [PMC free article] [PubMed] [Google Scholar]
  25. Wiggins G. L., Hollis D. G., Weaver R. Prevalence of serogroups and sulfonamide resistance of meningococci from the civilian population in the United States, 1964-1970. Am J Public Health. 1973 Jan;63(1):59–65. doi: 10.2105/ajph.63.1.59. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Wyle F. A., Artenstein M. S., Brandt B. L., Tramont E. C., Kasper D. L., Altieri P. L., Berman S. L., Lowenthal J. P. Immunologic response of man to group B meningococcal polysaccharide vaccines. J Infect Dis. 1972 Nov;126(5):514–521. doi: 10.1093/infdis/126.5.514. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES