Abstract
A method for purification of marble spleen disease virus (MSDV) from the spleens of infected turkeys and pheasants is described. It combines chloroform or fluorocarbon extraction with subsequent purification by centrifugation on a cushion of cesium chloride (CsCl). Further purification of MSDV was accomplished with a CsCl equilibrium density gradient. A viral buoyant density of approximately 1.32 to 1.33 g/cm3 was determined. Negative-stain electron microscopy revealed that virus isopycnically banded by CsCl gradient had non-enveloped icosahedral capsids composed of 252 capsomeres. The direct colorimetric diphenylamine assay indicated that MSDV has deoxyribonucleic acid as its nucleic acid. The above evidence demonstrates that MSDV is an avian adenovirus, the first recognized in the ring-necked pheasant (Phasianus colchicus L.).
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- BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Carlson H. C., Pettit J. R., Hemsley R. V., Mitchell W. R. Marble spleen disease of pheasants in Ontario. Can J Comp Med. 1973 Jul;37(3):281–286. [PMC free article] [PubMed] [Google Scholar]
- Fadly A. M., Winterfield R. W. Isolation and some characteristics of an agent associated with inclusion body hepatitis, hemorrhages, and aplastic anemia in chickens. Avian Dis. 1973 Jan-Mar;17(1):182–193. [PubMed] [Google Scholar]
- Gross W. B., Moore W. E. Hemorrhagic enteritis of turkeys. Avian Dis. 1967 May;11(2):296–307. [PubMed] [Google Scholar]
- Iltis J. P., Daniels S. B., Wyand D. S. Demonstration of an avian adenovirus as the causative agent of marble spleen disease. Am J Vet Res. 1977 Jan;38(1):95–100. [PubMed] [Google Scholar]
- Iltis J. P., Jakowski R. M., Wyand D. S. Experimentally transmitted marble spleen disease in pen-raised wild turkeys. J Wildl Dis. 1975 Oct;11(4):484–485. doi: 10.7589/0090-3558-11.4.484. [DOI] [PubMed] [Google Scholar]
- Iltis J. P., Jakowski R. M., Wyand D. S. Transmission of marble spleen disease in turkeys and pheasants. Am J Vet Res. 1975 Jan;36(1):97–101. [PubMed] [Google Scholar]
- Iltis J. P., Wyand D. S. Indications of a viral etiology for marble spleen disease in pheasants. J Wildl Dis. 1974 Jul;10(3):272–278. doi: 10.7589/0090-3558-10.3.272. [DOI] [PubMed] [Google Scholar]
- Jasty V., Chang P. W., Miller L. T., Yates V. J. Chicken embryo lethal orphan virus: virus localization and histopathology in chicken embryos. Avian Dis. 1969 Aug;13(3):519–527. [PubMed] [Google Scholar]
- Laver W. G., Younghusband H. B., Wrigley N. G. Purification and properties of chick embryo lethal orphan virus (an avian adenovirus). Virology. 1971 Sep;45(3):598–614. doi: 10.1016/0042-6822(71)90175-9. [DOI] [PubMed] [Google Scholar]
- McFerran J. B., Adair B., Connor T. J. Adenoviral antigens (CELO, QBV, GAL). Am J Vet Res. 1975 Apr;36(4 Pt 2):527–529. [PubMed] [Google Scholar]
- Norrby E. The relationship between the soluble antigens and the virion of adenovirus type 3. I. Morphological characteristics. Virology. 1966 Feb;28(2):236–248. doi: 10.1016/0042-6822(66)90148-6. [DOI] [PubMed] [Google Scholar]
- Wyand D. S., Jakowski R. M., Burke C. N. Marble spleen disease in ring-necked pheasants: histology and ultrastructure. Avian Dis. 1972 Jan-Mar;16(2):319–329. [PubMed] [Google Scholar]