Abstract
The cell membranes of a T-strain of mycoplasma, obtained by ultrasonic disruption, were as effective as whole organisms in eliciting metabolism-inhibiting and complement-fixing antibodies. The soluble fraction separated from cell membranes by centrifugation at 35,000 X g showed a minor ability to elicit an antibody response as measured by metabolism inhibition and complement fixation tests. After a further centrifugation at 100,000 X g, the immunogenic activity of the soluble fraction was completely lost. Immunogenic determinants in mycoplasma membranes could also be demonstrated by adsorption tests; cell membranes were more effective than soluble fractions in adsorbing antibody capacity from the immune sera against whole cells. It has been shown by further experiments that cell membranes have at least two major antigenic determinants, which differ either in chemical nature or in capacity to adsorb and evoke antibodies, characterized by different serological behaviors.
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Selected References
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- Amar A., Rottem S., Razin S. Characterization of the mycoplasma membrane proteins. IV. Disposition of proteins in the membrane. Biochim Biophys Acta. 1974 Jun 13;352(2):228–244. doi: 10.1016/0005-2736(74)90214-4. [DOI] [PubMed] [Google Scholar]
- Fleischer S., Fleischer B., Stoeckenius W. Fine structure of lipid-depleted mitochondria. J Cell Biol. 1967 Jan;32(1):193–208. doi: 10.1083/jcb.32.1.193. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kenny G. E. Heat-lability and organic solvent-solubility of mycoplasma antigens. Ann N Y Acad Sci. 1967 Jul 28;143(1):676–681. doi: 10.1111/j.1749-6632.1967.tb27713.x. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Ne'eman Z., Kahane I., Kovartovsky J., Razin S. Characterization of the myoplasma membrane proteins. 3. Gel filtration and immunological characterization of Acholeplasma laidlawii membrane proteins. Biochim Biophys Acta. 1972 Apr 14;266(1):255–268. doi: 10.1016/0005-2736(72)90140-x. [DOI] [PubMed] [Google Scholar]
- Purcell R. H., Taylor-Robinson D., Wong D., Chanock R. M. Color test for the measurement of antibody to T-strain mycoplasmas. J Bacteriol. 1966 Jul;92(1):6–12. doi: 10.1128/jb.92.1.6-12.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rega A. F., Weed R. I., Reed C. F., Berg G. G., Rothstein A. Changes in the properties of human erythrocyte membrane protein after solubilization by butanol extraction. Biochim Biophys Acta. 1967 Oct 23;147(2):297–312. doi: 10.1016/0005-2795(67)90408-4. [DOI] [PubMed] [Google Scholar]
- Romano N., Scarlata G. Serological activity of lipids of a T strain of Mycoplasma. Infect Immun. 1974 Jun;9(6):1062–1065. doi: 10.1128/iai.9.6.1062-1065.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Romano N., Smith P. F., Mayberry W. R. Lipids of a T strain of Mycoplasma. J Bacteriol. 1972 Feb;109(2):565–569. doi: 10.1128/jb.109.2.565-569.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]