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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Methods. 2014 May 27;68(3):417–424. doi: 10.1016/j.ymeth.2014.05.007

Table 2.

Heat-shock promoters and conditions

Promoter Target Construct Protocol Assay Reference
Phsp-16.48 Pmyo-2 >mCherry-STOP> GFP::HIS-11 1hr heat shock at 34º visualization of nuclear GFP in 3hrs, strong at 15 hrs [15]
Phsp-16.41 Ppro-1<GFP-STOP<lacZ 2hr at 30º acetone fix and βGal staining after 24hr at 20º [16]
Phsp-16.2 Plim-7<GFP-STOP<RFP 2, 3 or 4 hr at 33º (best at 3hr) visualization of RFP after 24hr at 25º [16]
Phsp-16.2 Ppro-1<GFP-STOP<lacZ 2 hr at 33º visualization of βGal after 24hr at 20º [16]
Phsp16.41 Plet-858<mCherry-STOP< EGFP-bicistronic-fem-3cDNA 1hr at 33ºC visualization of EGFP at 4hr and assay at 24hr [29]