Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1977 Jun;16(3):910–914. doi: 10.1128/iai.16.3.910-914.1977

Correlation between oral toxicity and in vitro stability of Clostridium botulinum type A and B toxins of different molecular sizes.

S Sugii, I Ohishi, G Sakaguchi
PMCID: PMC421049  PMID: 19355

Abstract

The in vitro sensitivity to acid and pepsin differed markedly among Clostridium botulinum type A and B toxins of different molecular sizes. The larger the molecular size of the toxin, the higher the resistance to these agents. Tye B derivative toxin was rapidly inactivated, but the progenitor toxins resisted in vitro exposure to rat intestinal juice. The molecular dissociation of the progenitor toxins did not occur in rat intestinal juice of pH 7.0, but did occur in a buffer solution of the same pH. The oral toxicity may depend mostly on the stability of toxin molecules in the stomach and, to a less extent, in the intestine. The present results seem to justify the conclusion that C. botulinum type A and B progenitor toxins with molecular sizes larger than 16S are more potent oral toxins than 12S progenitor toxins.

Full text

PDF
910

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. COLEMAN I. W. Studies on the oral toxicity of Clostridium botulinum toxin, type A. Can J Biochem Physiol. 1954 Jan;32(1):27–34. [PubMed] [Google Scholar]
  2. DUFF J. T., WRIGHT G. G., KLERER J., MOORE D. E., BIBLER R. H. Studies on immunity to toxins of Clostridium botulinum. I. A simplified procedure for isolation of type A toxin. J Bacteriol. 1957 Jan;73(1):42–47. doi: 10.1128/jb.73.1.42-47.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. HALLIWELL G. The action of proteolytic enzymes on Clostridium botulinum type A toxin. Biochem J. 1954 Sep;58(1):4–8. doi: 10.1042/bj0580004. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kitamura M., Sakaguchi S., Sakaguchi G. Dissociation of Clostridium botulinum type-E-toxin. Biochem Biophys Res Commun. 1967 Dec 29;29(6):892–897. doi: 10.1016/0006-291x(67)90305-1. [DOI] [PubMed] [Google Scholar]
  5. Kitamura M., Sakaguchi S., Sakaguchi G. Significance of 12S toxin of Clostridium botulinum type E. J Bacteriol. 1969 Jun;98(3):1173–1178. doi: 10.1128/jb.98.3.1173-1178.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kozaki S., Sakaguchi S., Sakaguchi G. Purification and some properties of progenitor toxins of Clostridium botulinum type B. Infect Immun. 1974 Oct;10(4):750–756. doi: 10.1128/iai.10.4.750-756.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kozaki S., Sugii S., Oishi I., Sakaguchi S., Sakaguchi G. Proceedings: Clostridium botulinum type A, B, E and F 12S toxins. Jpn J Med Sci Biol. 1975 Feb;28(1):70–72. [PubMed] [Google Scholar]
  8. Meyer E. A., Lamanna C. ACTIVITY OF TYPE A BOTULINAL TOXIN AND HEMAGGLUTININ EXPOSED TO PROTEOLYTIC ENZYME. J Bacteriol. 1959 Aug;78(2):175–180. doi: 10.1128/jb.78.2.175-180.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ohishi I., Sakaguchi G. Molecular construction of Clostridium botulinum type F progenitor toxin. Appl Microbiol. 1975 Apr;29(4):444–447. doi: 10.1128/am.29.4.444-447.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Ohishi I., Sugii S., Sakaguchi G. Oral toxicities of Clostridium botulinum toxins in response to molecular size. Infect Immun. 1977 Apr;16(1):107–109. doi: 10.1128/iai.16.1.107-109.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Sakaguchi G., Sakaguchi S. Oral toxicities of Clostridium botulinum type E toxins of different forms. Jpn J Med Sci Biol. 1974 Aug;27(4):241–244. doi: 10.7883/yoken1952.27.241. [DOI] [PubMed] [Google Scholar]
  12. Sugii S., Sakaguchi G. Molecular construction of Clostridium botulinum type A toxins. Infect Immun. 1975 Dec;12(6):1262–1270. doi: 10.1128/iai.12.6.1262-1270.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sugiyama H., DasGupta B. R., Yang K. H. Toxicity of purified botulinal toxin fed to mice. Proc Soc Exp Biol Med. 1974 Nov;147(2):589–591. doi: 10.3181/00379727-147-38394. [DOI] [PubMed] [Google Scholar]
  14. WAGMAN J., BATEMAN J. B. Botulinum type A toxin: properties of a toxic dissociation product. Arch Biochem Biophys. 1953 Aug;45(2):375–383. doi: 10.1016/s0003-9861(53)80014-7. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES