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. 1977 Jun;16(3):974–982. doi: 10.1128/iai.16.3.974-982.1977

Role of lysozyme in the microbicidal activity of rat alveolar macrophages.

W D Biggar, J M Sturgess
PMCID: PMC421060  PMID: 561033

Abstract

Lysozyme release from alveolar macrophages is stimulated by exposure to particles, such as latex and zymosan, and to bacteria. Rat alveolar marcophages contain 10-fold-greater intracellualr concentrations of lysozyme and release more lysozyme after stimulation than rat blood neutrophils. During 30 min of incubation in vitro, alveolar macrophages kill more than 99% of Micrococcus lysodeikticus in the incubation mixture, whereas neutrophils kill approximately 50% of the bacteria. The bactericidal capacity of alveolar macrophages for M. lysodeikticus exceeds that of neutrophils at all bacteria-to-cell ratios tested. This bacterial killing by alveolar macrophages is inhibited when specific rabbit antirat lysozyme serum is added to the incubation mixture. Electron microscopy studies indicate that bacterial killing occurs extracellularly. Initial degradation of bacteria occurs within 5 min, and lysis is complete by 25 to 30 min. Phagocytosis of lysed bacteria is maximum after 25 to 30 min. The greater quantities of lysozyme, both intracellularly and released into the extracellular environment by alveolar macrophages, suggest that this factor may be a mechanism by which alveolar macrophages contribute to pulmonary defense.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Biggar W. D., Buron S., Holmes B. Bactericidal mechanisms in rabbit alveolar macrophages: evidence against peroxidase and hydrogen peroxide bactericidal mechanisms. Infect Immun. 1976 Jul;14(1):6–10. doi: 10.1128/iai.14.1.6-10.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Biggar W. D., Holmes B., Good R. A. Opsonic defect in patients with cystic fibrosis of the pancreas. Proc Natl Acad Sci U S A. 1971 Aug;68(8):1716–1719. doi: 10.1073/pnas.68.8.1716. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Biggar W. D., Sturgess J. M. Peroxidase activity of alveolar macrophages. Lab Invest. 1976 Jan;34(1):31–42. [PubMed] [Google Scholar]
  4. Chodirker W. B., Bock G. N., Vaughan J. H. Isolation of human PMN leukocytes and granules: observations on early blood diluion and on heparin. J Lab Clin Med. 1968 Jan;71(1):9–19. [PubMed] [Google Scholar]
  5. GREEN G. M., KASS E. H. THE ROLE OF THE ALVEOLAR MACROPHAGE IN THE CLEARANCE OF BACTERIA FROM THE LUNG. J Exp Med. 1964 Jan 1;119:167–176. doi: 10.1084/jem.119.1.167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Glynn A. A. Lysozyme: antigen, enzyme and antibacterial agent. Sci Basis Med Annu Rev. 1968:31–52. [PubMed] [Google Scholar]
  7. Gordon S., Todd J., Cohn Z. A. In vitro synthesis and secretion of lysozyme by mononuclear phagocytes. J Exp Med. 1974 May 1;139(5):1228–1248. doi: 10.1084/jem.139.5.1228. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Green G. M. The J. Burns Amberson Lecture--in defense of the lung. Am Rev Respir Dis. 1970 Nov;102(5):691–703. doi: 10.1164/arrd.1970.102.5.691. [DOI] [PubMed] [Google Scholar]
  9. Hawkins D., Peeters S. The response of polymorphonuclear leukocytes to immune complexes in vitro. Lab Invest. 1971 Jun;24(6):483–491. [PubMed] [Google Scholar]
  10. Hill I. R., Porter P. Studies of bactericidal activity to Escherichia coli of porcine serum and colostral immunoglobulins and the role of lysozyme with secretory IgA. Immunology. 1974 Jun;26(6):1239–1250. [PMC free article] [PubMed] [Google Scholar]
  11. Karnovsky M. L. Chronic granulomatous disease--pieces of a cellular and molecular puzzle. Fed Proc. 1973 Apr;32(4):1527–1533. [PubMed] [Google Scholar]
  12. Lahav M., Ne'eman N., Adler E., Ginsburg I. Effect of leukocyte hydrolases on bacteria. I. Degradation of 14C-labeled Streptococcus and Staphylococcus by leukocyte lysates in vitro. J Infect Dis. 1974 May;129(5):528–537. doi: 10.1093/infdis/129.5.528. [DOI] [PubMed] [Google Scholar]
  13. MYRVIK Q. N., LEAKE E. S., FARISS B. Lysozyme content of alveolar and peritoneal macrophages from the rabbit. J Immunol. 1961 Feb;86:133–136. [PubMed] [Google Scholar]
  14. MYRVIK Q., LEAKE E. S., FARISS B. Studies on pulmonary alveolar macrophages from the normal rabbit: a technique to procure them in a high state of purity. J Immunol. 1961 Feb;86:128–132. [PubMed] [Google Scholar]
  15. McClelland D. B., van Furth R. In vitro synthesis of lysozyme by human and mouse tissues and leucocytes. Immunology. 1975 Jun;28(6):1099–1114. [PMC free article] [PubMed] [Google Scholar]
  16. WARDLAW A. C. The complement-dependent bacteriolytic activity of normal human serum. I. The effect of pH and ionic strength and the role of lysozyme. J Exp Med. 1962 Jun 1;115:1231–1249. doi: 10.1084/jem.115.6.1231. [DOI] [PMC free article] [PubMed] [Google Scholar]

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