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. 1977 Aug;17(2):282–285. doi: 10.1128/iai.17.2.282-285.1977

Production by mixed lymphocyte cultures of a type II interferon able to protect macrophages against virus infection.

J L Virelizier, A C Allison, E de Maeyer
PMCID: PMC421115  PMID: 197017

Abstract

In supernatants of mixed mouse spleen cell cultures established for 4 days, a species-specific inhibitor of virus replication with a broad antiviral spectrum was found. The inhibitor was destroyed by trypsin, was nondialyzable and acid labile, and was not neutralized by antibody to mouse L cell interferon. This indicates that in mixed lymphocyte cultures a type II interferon is made that has no immunological relationship with "fibroblast" interferon. This leukocyte product was shown to protect mouse hepatitis viruses. It is suggested that lymphocyte interferon may collaborate with macrophages in host defense against viruses, as a mediator of cellular immunity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allison A. C. On the role of mononuclear phagocytes in immunity against viruses. Prog Med Virol. 1974;18(0):15–31. [PubMed] [Google Scholar]
  2. Berg K., Ogburn C. A., Paucker K., Mogensen K. E., Cantell K. Affinity chromatography of human leukocyte and diploid cell interferons on sepharose-bound antibodies. J Immunol. 1975 Feb;114(2 Pt 1):640–644. [PubMed] [Google Scholar]
  3. De Maeyer E., De Maeyer-Guignard J., Jullien P. Inter- feron synthesis in x-irradiated animals. 3. The high radiosensitivity of myxovirus-induced circulating interferon production. Proc Soc Exp Biol Med. 1969 May;131(1):36–41. doi: 10.3181/00379727-131-33799. [DOI] [PubMed] [Google Scholar]
  4. Gifford G. E., Tibor A., Peavy D. L. Interferon production in mixed lymphocyte cell cultures. Infect Immun. 1971 Jan;3(1):164–166. doi: 10.1128/iai.3.1.164-166.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Green J. A., Cooperband S. R., Kibrick S. Immune specific induction of interferon production in cultures of human blood lymphocytes. Science. 1969 Jun 20;164(3886):1415–1417. doi: 10.1126/science.164.3886.1415. [DOI] [PubMed] [Google Scholar]
  6. Haller O., Arnheiter H., Lindenmann J. Genetically determined resistance to infection by hepatotropic influenza A virus in mice: effect of immunosuppression. Infect Immun. 1976 Mar;13(3):844–854. doi: 10.1128/iai.13.3.844-854.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Havell E. A., Berman B., Ogburn C. A., Berg K., Paucker K., Vilcek J. Two antigenically distinct species of human interferon. Proc Natl Acad Sci U S A. 1975 Jun;72(6):2185–2187. doi: 10.1073/pnas.72.6.2185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Le Prevost C., Levy-Leblond E., Virelizier J. L., Dupuy J. M. Immunopathology of mouse hepatitis virus type 3 infection. Role of humoral and cell-mediated immunity in resistance mechanisms. J Immunol. 1975 Jan;114(1 Pt 1):221–225. [PubMed] [Google Scholar]
  9. Rager-Zisman B., Allison A. C. Mechanism of immunologic resistance to herpes simplex virus 1 (HSV-1) infection. J Immunol. 1976 Jan;116(1):35–40. [PubMed] [Google Scholar]
  10. Rasmussen L. E., Jordan G. W., Stevens D. A., Merigan T. C. Lymphocyte interferon production and transformation after Herpes simplex infections in humans. J Immunol. 1974 Feb;112(2):728–736. [PubMed] [Google Scholar]
  11. Sipe J. D., de Maeyer-Guignard J., Fauconnier B., de Maeyer E. Purification of mouse interferon by affinity chromatography on a solid-phase immunoadsorbent. Proc Natl Acad Sci U S A. 1973 Apr;70(4):1037–1040. doi: 10.1073/pnas.70.4.1037. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Valle M. J., Bobrove A. M., Strober S., Merigan T. C. Immune specific production of interferon by human T cells in combined macrophage-lymphocyte cultures in response to Herpes simplex antigen. J Immunol. 1975 Jan;114(1 Pt 2):435–441. [PubMed] [Google Scholar]
  13. Virelizier J. L., Allison A. C. Correlation of persistent mouse hepatitis virus (MHV-3) infection with its effect on mouse macrophage cultures. Arch Virol. 1976;50(4):279–285. doi: 10.1007/BF01317953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Virelizier J. L., Dayan A. D., Allison A. C. Neuropathological effects of persistent infection of mice by mouse hepatitis virus. Infect Immun. 1975 Nov;12(5):1127–1140. doi: 10.1128/iai.12.5.1127-1140.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Virelizier J. L., Virelizier A. M., Allison A. C. The role of circulating interferon in the modifications of immune responsiveness by mouse hepatitis virus (MHV-3). J Immunol. 1976 Sep;117(3):748–753. [PubMed] [Google Scholar]
  16. Youngner J. S., Salvin S. B. Production and properties of migration inhibitory factor and interferon in the circulation of mice with delayed hypersensitivity. J Immunol. 1973 Dec;111(6):1914–1922. [PubMed] [Google Scholar]

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