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. 1977 Aug;17(2):366–370. doi: 10.1128/iai.17.2.366-370.1977

Local immune response to lipoprotein of the outer membrane of Escherichia coli in experimental pyelonephritis.

J W Smith
PMCID: PMC421129  PMID: 330406

Abstract

The local immune response to the lipoprotein of the outer membrane of Escherichia coli O6:K13:H1 was determined in experimental hematogenous pyelonephritis in rabbits. Local antibody was analyzed with the enzyme-linked immunosorbent assay on newly synthesized protein from kidney. Local or intrarenal antibody was detected by day 7 of infection, a few days later than serum antibody. The synthesis of antibody in immunoglobulin G class was present 6 days before the synthesis of immunoglobulin M antibody. Antibody activity to this antigen was also present in urine, and antibody could be eluted from bacteria coated with antibody. Local antibody to the lipoprotein was synthesized in equal quantities by animals infected with a heterologous species of E. coli. This antigen was not as mitogenic for splenic or kidney lymphocytes as was the O antigen. Hence, the lipoprotein of the organisms is a potent immunogen but a weak mitogen locally in pyelonephritis. This antibody response probably does not induce protection against infection, but represents a marker for presence of infection.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Braun V., Bosch V., Klumpp E. R., Neff I., Mayer H., Schlecht S. Antigenic determinants of murein lipoprotein and its exposure at the surface of Enterobacteriaceae. Eur J Biochem. 1976 Mar 1;62(3):555–566. doi: 10.1111/j.1432-1033.1976.tb10190.x. [DOI] [PubMed] [Google Scholar]
  2. Braun V., Rehn K. Chemical characterization, spatial distribution and function of a lipoprotein (murein-lipoprotein) of the E. coli cell wall. The specific effect of trypsin on the membrane structure. Eur J Biochem. 1969 Oct;10(3):426–438. doi: 10.1111/j.1432-1033.1969.tb00707.x. [DOI] [PubMed] [Google Scholar]
  3. Braun V., Wolff H. The murein-lipoprotein linkage in the cell wall of Escherichia coli. Eur J Biochem. 1970 Jun;14(2):387–391. doi: 10.1111/j.1432-1033.1970.tb00301.x. [DOI] [PubMed] [Google Scholar]
  4. Hand W. L., Smith J. W., Miller T. E., Barnett J. A., Sanford J. P. Immunoglobulin synthesis in lower urinary tract infection. J Lab Clin Med. 1970 Jan;75(1):19–29. [PubMed] [Google Scholar]
  5. Holmgren J., Smith J. W. Immunological aspects of urinary tract infections. Prog Allergy. 1975;18:289–352. [PubMed] [Google Scholar]
  6. Jones S. R., Johnson J. Further evaluation of the test for detection of antibody-coated bacteria in urine sediment. J Clin Microbiol. 1977 May;5(5):510–513. doi: 10.1128/jcm.5.5.510-513.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lehmann J. D., Smith J. W., Miller T. E., Barnett J. A., Sanford J. P. Local immune response in experimental pyelonephritis. J Clin Invest. 1969 Nov;47(11):2541–2550. doi: 10.1172/JCI105936. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Melchers F., Braun V., Galanos C. The lipoprotein of the outer membrane of Escherichia coli: a B-lymphocyte mitogen. J Exp Med. 1975 Aug 1;142(2):473–482. doi: 10.1084/jem.142.2.473. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. OSBORN M. J. STUDIES ON THE GRAM-NEGATIVE CELL WALL. I. EVIDENCE FOR THE ROLE OF 2-KETO- 3-DEOXYOCTONATE IN THE LIPOPOLYSACCHARIDE OF SALMONELLA TYPHIMURIUM. Proc Natl Acad Sci U S A. 1963 Sep;50:499–506. doi: 10.1073/pnas.50.3.499. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Schnaitman C. A. Protein composition of the cell wall and cytoplasmic membrane of Escherichia coli. J Bacteriol. 1970 Nov;104(2):890–901. doi: 10.1128/jb.104.2.890-901.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Smith J. W., Adkins M. J., McCreary D. Local immune response in experimental pyelonephritis in the rabbit. I. Morphological and functional features of the lymphocytic infiltrate. Immunology. 1975 Dec;29(6):1067–1076. [PMC free article] [PubMed] [Google Scholar]
  12. Smith J. W., Jones S. R., Kaijser B. Significance of antibody-coated bacteria in urinary sediment in experimental pyelonephritis. J Infect Dis. 1977 Apr;135(4):577–581. doi: 10.1093/infdis/135.4.577. [DOI] [PubMed] [Google Scholar]
  13. Smith J. W., Kaijser B. Local immunie response in experimental pyelonephritis in the rabbit. III. Lymphocyte responsivieness to O and K antigens of Escherichia coli. Immunology. 1976 Aug;31(2):233–237. [PMC free article] [PubMed] [Google Scholar]
  14. Smith J. W., Kaijser B. The local immune response to Escherichia coli O and K antigen in experimental pyelonephritis. J Clin Invest. 1976 Aug;58(2):276–281. doi: 10.1172/JCI108469. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Smith J. W. Local immune response in experimental pyelonephritis in the rabbit. II. Lymphocyte stimulation by lipopolysaccharide of infecting organism. Immunology. 1975 Dec;29(6):1077–1085. [PMC free article] [PubMed] [Google Scholar]
  16. Smith J., Holmgren J., Ahlstedt S., Hanson L. A. Local antibody production in experimental pyelonephritis: amount, avidity, and immunoglobulin class. Infect Immun. 1974 Sep;10(3):411–415. doi: 10.1128/iai.10.3.411-415.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. UHR J. W. THE HETEROGENEITY OF THE IMMUNE RESPONSE. Science. 1964 Jul 31;145(3631):457–464. doi: 10.1126/science.145.3631.457. [DOI] [PubMed] [Google Scholar]

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