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. 1977 Sep;17(3):599–606. doi: 10.1128/iai.17.3.599-606.1977

Cytotoxicity of rat macrophages activated by persistent or biodegradable bacterial cell walls.

R J Smialowicz, J H Schwab
PMCID: PMC421169  PMID: 409679

Abstract

Peritoneal macrophages from Fischer 344 rats were cytotoxic for mouse fibroblasts after in vitro phagocytosis of non-biodegradable group A streptococcal cell walls. In contrast, macrophages from Buffalo rats were not activated to cytotoxicity by bacterial cell walls. Activation to cytotoxicity was only achieved by the macrophages after an interval of at least 3 days in culture after ingestion of group A cell walls. If macrophages were cultured only 24 h after ingestion of cell walls, a cytostatic effect on target cells was observed. Phagocytosis of biodegradable group D streptococcal cell walls did not activate macrophages from either rat strain to become cytotoxic. No direct toxicity of cell walls for macrophages or mouse L-cells was observed. The cytotoxicity of Fischer 344 macrophages appears to require direct cell contact with target cells.

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Selected References

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  1. Alexander P., Evans R. Endotoxin and double stranded RNA render macrophages cytotoxic. Nat New Biol. 1971 Jul 21;232(29):76–78. doi: 10.1038/newbio232076a0. [DOI] [PubMed] [Google Scholar]
  2. Bruley-Rosset M., Florentin I., Khalil A. M., Mathé G. Nonspecific macrophage activation by systemic adjuvants. Evaluation by lysosomal enzyme and in vitro tumoricidal activities. Int Arch Allergy Appl Immunol. 1976;51(5):594–607. doi: 10.1159/000231638. [DOI] [PubMed] [Google Scholar]
  3. Cromartie W. J., Craddock J. G. Rheumatic-like cardiac lesions in mice. Science. 1966 Oct 14;154(3746):285–287. doi: 10.1126/science.154.3746.285. [DOI] [PubMed] [Google Scholar]
  4. Davies P., Page R. C., Allison A. C. Changes in cellular enzyme levels and extracellular release of lysosomal acid hydrolases in macrophages exposed to group A streptococcal cell wall substance. J Exp Med. 1974 May 1;139(5):1262–1282. doi: 10.1084/jem.139.5.1262. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gordon S., Todd J., Cohn Z. A. In vitro synthesis and secretion of lysozyme by mononuclear phagocytes. J Exp Med. 1974 May 1;139(5):1228–1248. doi: 10.1084/jem.139.5.1228. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Heymer B., Haferkamp O. Reticuloendothelial system cells modifying bacterial antigen specificity. Med Microbiol Immunol. 1971;157(1):96–100. doi: 10.1007/BF02121295. [DOI] [PubMed] [Google Scholar]
  7. Hibbs J. B., Jr Heterocytolysis by macrophages activated by bacillus Calmette-Guérin: lysosome exocytosis into tumor cells. Science. 1974 Apr 26;184(4135):468–471. doi: 10.1126/science.184.4135.468. [DOI] [PubMed] [Google Scholar]
  8. Holtermann O. A., Klein E., Casale G. P. Selective cytotoxicity of peritoneal leucocytes for neoplastic cells. Cell Immunol. 1973 Dec;9(3):339–352. doi: 10.1016/0008-8749(73)90049-x. [DOI] [PubMed] [Google Scholar]
  9. Jones J. M., Schwab J. H. Effects of streptococcal cell wall fragments on phagocytosis and tissue culture cells. Infect Immun. 1970 Mar;1(3):232–242. doi: 10.1128/iai.1.3.232-242.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Keller R. Cytostatic elimination of syngeneic rat tumor cells in vitro by nonspecifically activated macrophages. J Exp Med. 1973 Sep 1;138(3):625–644. doi: 10.1084/jem.138.3.625. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. McLaughlin J. F., Ruddle N. H., Waksman B. H. Relationship between activation of peritoneal cells and their cytopathogenicity. J Reticuloendothel Soc. 1972 Sep;12(3):293–304. [PubMed] [Google Scholar]
  12. Nathan C. F., Karnovsky M. L., David J. R. Alterations of macrophage functions by mediators from lymphocytes. J Exp Med. 1971 Jun 1;133(6):1356–1376. doi: 10.1084/jem.133.6.1356. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ohanian S. H., Schwab J. H., Cromartie W. J. Relation of rheumatic-like cardiac lesions of the mouse to localization of group A streptococcal cell walls. J Exp Med. 1969 Jan 1;129(1):37–49. doi: 10.1084/jem.129.1.37. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Ohanian S. H., Schwab J. H. Persistence of group a streptococcal cell walls related to chronic inflammation of rabbit dermal connective tissue. J Exp Med. 1967 Jun 1;125(6):1137–1148. doi: 10.1084/jem.125.6.1137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Piessens W. F., Churchill W. H., Jr, David Macrophages activated in vitro with lymphocyte mediators kill neoplastic but not normal cells. J Immunol. 1975 Jan;114(1 Pt 2):293–299. [PubMed] [Google Scholar]
  16. Rickles N., Zilberstein Z., Kraus S., Arad G., Kaufstein M., Ginsburg I. Persistence of group A streptococci labeled with fluorescein isothiocyanate in inflammatory sites in the heart and muscle of mice and rabbits. Proc Soc Exp Biol Med. 1969 Jun;131(2):525–530. doi: 10.3181/00379727-131-33917. [DOI] [PubMed] [Google Scholar]
  17. SCHWAB J. H., CROMARTIE W. J. Studies on a toxic cellular component of group A streptococci. J Bacteriol. 1957 Nov;74(5):673–679. doi: 10.1128/jb.74.5.673-679.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Schwab J. H., Cromartie W. J., Ohanian S. H., Craddock J. G. Association of experimental chronic arthritis with the persistence of group A streptococcal cell walls in the articular tissue. J Bacteriol. 1967 Nov;94(5):1728–1735. doi: 10.1128/jb.94.5.1728-1735.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Smialowicz R. J., Schwab J. H. Processing of streptococcal cell walls by rat macrophages and human monocytes in vitro. Infect Immun. 1977 Sep;17(3):591–598. doi: 10.1128/iai.17.3.591-598.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Unkeless J. C., Gordon S., Reich E. Secretion of plasminogen activator by stimulated macrophages. J Exp Med. 1974 Apr 1;139(4):834–850. doi: 10.1084/jem.139.4.834. [DOI] [PMC free article] [PubMed] [Google Scholar]

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