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. 1977 Oct;18(1):46–51. doi: 10.1128/iai.18.1.46-51.1977

Cell-mediated immunity to tumor antigen in Marek's disease: susceptibility of effector cells to antithymocyte serum and enhancement of cytotoxic activity by Vibrio cholerae neuraminidase.

J M Sharma
PMCID: PMC421191  PMID: 302827

Abstract

Spleen cells from chickens inoculated 7 to 8 days previously with Marek's disease virus were cytotoxic for 51Cr-labeled cells of a Marek's disease lymphoblastoid cell line (MSB-1 line) in a 4-h in vitro cytotoxic assay. The cytotoxic activity of spleen cells was inhibited by pretreatment with antithymocyte serum and complement, but not with complement alone or in combination with anti-bursa cell serum or normal preimmune serum. The conclusion was that the effector cell in the above cytotoxic assay was a thymus-derived lymphocyte. Also, pretreatment of target cells with Vibrio cholerae neuraminidase enhanced in vitro cytotoxic activity of effector cells. Similar enzymatic treatment of effector cells had a negligible effect on cytotoxicity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Akiyama Y., Kato S. Two cell lines from lymphomas of Marek's disease. Biken J. 1974 Sep;17(3):105–116. [PubMed] [Google Scholar]
  2. Albini B., Wick G. Delineation of B and T lymphoid cells in the chicken. J Immunol. 1974 Feb;112(2):444–450. [PubMed] [Google Scholar]
  3. Cantor H., Boyse E. A. Functional subclasses of T lymphocytes bearing different Ly antigens. II. Cooperation between subclasses of Ly+ cells in the generation of killer activity. J Exp Med. 1975 Jun 1;141(6):1390–1399. doi: 10.1084/jem.141.6.1390. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cantor H., Boyse E. A. Functional subclasses of T-lymphocytes bearing different Ly antigens. I. The generation of functionally distinct T-cell subclasses is a differentiative process independent of antigen. J Exp Med. 1975 Jun 1;141(6):1376–1389. doi: 10.1084/jem.141.6.1376. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Colley D. G., Wu A. Y., Waksman B. H. Cellular differentiation in the thymus. 3. Surface properties of rat thymus and lymph node cells separated on density gradients. J Exp Med. 1970 Dec 1;132(6):1107–1121. doi: 10.1084/jem.132.6.1107. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Currie G. A., Bagshawe K. D. The role of sialic acid in antigenic expression: further studies of the Landschütz ascites tumour. Br J Cancer. 1968 Dec;22(4):843–853. doi: 10.1038/bjc.1968.99. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Currie G. A., Bagshawe K. D. Tumour specific immunogenicity of methylcholanthrene-induced sarcoma cells after incubation in neuraminidase. Br J Cancer. 1969 Mar;23(1):141–149. doi: 10.1038/bjc.1969.21. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Droege W. The antigen-inexperienced thymic suppressor cells: a class of lymphocytes in the young chicken thymus that inhibits antibody production and cell-mediated immune responses. Eur J Immunol. 1976 Apr;6(4):279–287. doi: 10.1002/eji.1830060409. [DOI] [PubMed] [Google Scholar]
  9. Droege W., Zucker R., Hannig K. Developmental changes in the cellular composition of the chicken thymus. Cell Immunol. 1974 May;12(2):186–193. doi: 10.1016/0008-8749(74)90071-9. [DOI] [PubMed] [Google Scholar]
  10. Drzeniek R. Differences in splitting capacity of virus and V. cholerae neuraminidases on sialic acid type substrates. Biochem Biophys Res Commun. 1967 Mar 21;26(6):631–638. doi: 10.1016/s0006-291x(67)80118-9. [DOI] [PubMed] [Google Scholar]
  11. Fernando W. W., Calnek B. W. Influence of bursa of Fabricius on infection and pathological response of chickens exposed to Marek's disease herpesvirus. Avian Dis. 1971 Jul-Sep;15(3):467–476. [PubMed] [Google Scholar]
  12. Han T., Pauly J. Synergism of stimulating capacity of pooled lymphocytes in the mixed lymphocyte reaction. Immunology. 1973 May;24(5):895–899. [PMC free article] [PubMed] [Google Scholar]
  13. Hudson L., Payne L. N. An analysis of the T and B cells of Marek's disease lymphomas of the chicken. Nat New Biol. 1973 Jan 10;241(106):52–53. doi: 10.1038/newbio241052a0. [DOI] [PubMed] [Google Scholar]
  14. Kisielow P., Hirst J. A., Shiku H., Beverley P. C., Hoffman M. K., Boyse E. A., Oettgen H. F. Ly antigens as markers for functionally distinct subpopulations of thymus-derived lymphocytes of the mouse. Nature. 1975 Jan 17;253(5488):219–220. doi: 10.1038/253219a0. [DOI] [PubMed] [Google Scholar]
  15. Nazerian K., Sharma J. M. Detection of T-cell surface antigens in a Marek's disease lymphoblastoid cell line. J Natl Cancer Inst. 1975 Jan;54(1):277–279. doi: 10.1093/jnci/54.1.277. [DOI] [PubMed] [Google Scholar]
  16. Nazerian K., Witter R. L. Properties of a chicken lymphoblastoid cell line from Marek's disease tumor. J Natl Cancer Inst. 1975 Feb;54(2):453–458. [PubMed] [Google Scholar]
  17. Phillips P. A., Biggs P. M. Course of infection in tissues of susceptible chickens after exposure to strains of Marek's disease virus and turkey herpesvirus. J Natl Cancer Inst. 1972 Nov;49(5):1367–1373. [PubMed] [Google Scholar]
  18. Powell P. C. Immunity to Marek's disease induced by glutaraldehyde-treated cells of Marek's disease lymphoblastoid cell lines. Nature. 1975 Oct 23;257(5528):684–685. doi: 10.1038/257684a0. [DOI] [PubMed] [Google Scholar]
  19. Powell P. C., Payne L. N., Frazier J. A., Rennie M. T lymphoblastoid cell lines from Marek's disease lymphomas. Nature. 1974 Sep 6;251(5470):79–80. doi: 10.1038/251079a0. [DOI] [PubMed] [Google Scholar]
  20. Powell P. C. Studies on Marek's disease lymphoma-derived cell lines. Bibl Haematol. 1975 Oct;(43):348–350. doi: 10.1159/000399163. [DOI] [PubMed] [Google Scholar]
  21. Ray P. K., Gewurz H., Simmons R. L. The serologic behaviour of neuraminidase-treated lymphoid cells. Alloantigenicity and complement sensitivity. Clin Exp Immunol. 1972 Jul;11(3):441–460. [PMC free article] [PubMed] [Google Scholar]
  22. Sanford B. H., Codington J. F. Further studies on the effect of neuraminidase on tumor cell transplantability. Tissue Antigens. 1971;1(4):153–161. doi: 10.1111/j.1399-0039.1971.tb00092.x. [DOI] [PubMed] [Google Scholar]
  23. Schlesinger M., Amos B. D. Effect of neuraminidase on serological properties of murine lymphoid cells. Transplant Proc. 1971 Mar;3(1):895–897. [PubMed] [Google Scholar]
  24. Sharma J. M., Coulson B. D. Cell-mediated cytotoxic response to cells bearing Marek's disease tumor-associated surface antigen in chickens infected with Marek's disease virus. J Natl Cancer Inst. 1977 Jun;58(6):1647–1651. doi: 10.1093/jnci/58.6.1647. [DOI] [PubMed] [Google Scholar]
  25. Sharma J. M., Nazerian K., Witter R. L. Reduced incidence of Marek's disease gross lymphomas in T-cell-depleted chickens. J Natl Cancer Inst. 1977 Mar;58(3):689–692. doi: 10.1093/jnci/58.3.689. [DOI] [PubMed] [Google Scholar]
  26. Sharma J. M. Resistance to Marek's disease in immunologically deficient chickens. Nature. 1974 Jan 11;247(5436):117–118. doi: 10.1038/247117a0. [DOI] [PubMed] [Google Scholar]
  27. Sharma J. M., Witter R. L., Purchase H. G. Absence of age-resistance in neonatally thymectomised chickens as evidence for cell-mediated immune surveillance in Marek's disease. Nature. 1975 Feb 6;253(5491):477–479. doi: 10.1038/253477a0. [DOI] [PubMed] [Google Scholar]
  28. Sharma J. M., Witter R. L. The effect of B-cell immunosuppression on age-related resistance of chickens to Marek's disease. Cancer Res. 1975 Mar;35(3):711–717. [PubMed] [Google Scholar]
  29. Shiku H., Kisielow P., Bean M. A., Takahashi T., Boyse E. A., Oettgen H. F., Old L. J. Expression of T-cell differentiation antigens on effector cells in cell-mediated cytotoxicity in vitro. Evidence for functional heterogeneity related to the surface phenotype of T cells. J Exp Med. 1975 Jan 1;141(1):227–241. doi: 10.1084/jem.141.1.227. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Siegfried L. M., Olson C. Characteristics of avian transmissible lymphoid tumor cells maintained in culture. J Natl Cancer Inst. 1972 Mar;48(3):791–795. [PubMed] [Google Scholar]
  31. Witter R. L., Stephens E. A., Sharma J. M., Nazerian K. Demonstration of a tumor-associated surface antigen in Marek's disease. J Immunol. 1975 Jul;115(1):177–183. [PubMed] [Google Scholar]
  32. Zucker R. M., Helfman D. M. Thymus subpopulations: density gradients and volume distributions studies. Cell Immunol. 1976 Mar 1;22(1):43–52. doi: 10.1016/0008-8749(76)90005-8. [DOI] [PubMed] [Google Scholar]

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