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. 1977 Oct;18(1):165–172. doi: 10.1128/iai.18.1.165-172.1977

Genetic studies on Neisseria gonorrhoeae from disseminated gonococcal infections.

L W Mayer, G K Schoolnik, S Falkow
PMCID: PMC421209  PMID: 409684

Abstract

Isolates from uncomplicated and disseminated gonococcal infections were analyzed by using deoxyribonucleic acid-mediated transformation. Most pairs of auxotrophs could recombine, producing independent transformants. When the constellation of arginine (Arg), hypoxanthine (Hyx), and uracil (Ura) requirements was present in donor and recipient, no recombination for these traits could be detected. Except for Arg to Hyx, no linkage between Arg, Hyx, Ura, penicillin G sensitivity, and serum resistance could be demonstrated. Some distant linkage of Ura to nalidixic acid and rifampin resistances was found. The data show that the traits associated with disseminated gonococcal infection strains are not closely linked but are identical in all strains, indicating a common origin.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bachmann B. J., Low K. B., Taylor A. L. Recalibrated linkage map of Escherichia coli K-12. Bacteriol Rev. 1976 Mar;40(1):116–167. doi: 10.1128/br.40.1.116-167.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bumgarner L. R., Finkelstein R. A. Pathogenesis and immunology of experimental gonococcal infection: virulence of colony types of Neisseria gonorrhoeae for chicken embryos. Infect Immun. 1973 Dec;8(6):919–924. doi: 10.1128/iai.8.6.919-924.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CATLIN B. W. Transformation of Neisseria meningitidis by deoxyribonucleates from cells and from culture slime. J Bacteriol. 1960 Apr;79:579–590. doi: 10.1128/jb.79.4.579-590.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Carifo K., Catlin B. W. Neisseria gonorrhoeae auxotyping: differentiation of clinical isolates based on growth responses on chemically defined media. Appl Microbiol. 1973 Sep;26(3):223–230. doi: 10.1128/am.26.3.223-230.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Catlin B. W. Genetic transformation of biosynthetically defective Neisseria gonorrhoeae clinical isolates. J Bacteriol. 1974 Oct;120(1):203–209. doi: 10.1128/jb.120.1.203-209.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Catlin B. W. Nutritional profiles of Neisseria gonorrhoeae, Neisseria meningitidis, and Neisseria lactamica in chemically defined media and the use of growth requirements for gonococcal typing. J Infect Dis. 1973 Aug;128(2):178–194. doi: 10.1093/infdis/128.2.178. [DOI] [PubMed] [Google Scholar]
  7. Cayeux P., Acar J. F., Chabbert Y. A. Bacterial persistence in streptococcal endocarditis due to thiol-requiring mutants. J Infect Dis. 1971 Sep;124(3):247–254. doi: 10.1093/infdis/124.3.247. [DOI] [PubMed] [Google Scholar]
  8. Holmes K. K., Counts G. W., Beaty H. N. Disseminated gonococcal infection. Ann Intern Med. 1971 Jun;74(6):979–993. doi: 10.7326/0003-4819-74-6-979. [DOI] [PubMed] [Google Scholar]
  9. KELLOGG D. S., Jr, PEACOCK W. L., Jr, DEACON W. E., BROWN L., PIRKLE D. I. NEISSERIA GONORRHOEAE. I. VIRULENCE GENETICALLY LINKED TO CLONAL VARIATION. J Bacteriol. 1963 Jun;85:1274–1279. doi: 10.1128/jb.85.6.1274-1279.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Knapp J. S., Holmes K. K. Disseminated gonococcal infections caused by Neisseria gonorrhoeae with unique nutritional requirements. J Infect Dis. 1975 Aug;132(2):204–208. doi: 10.1093/infdis/132.2.204. [DOI] [PubMed] [Google Scholar]
  11. La Scolea L. J., Jr, Dul M. J., Young F. E. Stability of pathogenic colony types of Neisseria gonorrhoeae in liquid culture by using the parameters of colonial morphology and deoxyribonucleic acid transformation. J Clin Microbiol. 1975 Feb;1(2):165–170. doi: 10.1128/jcm.1.2.165-170.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Maness M. J., Sparling P. F. Multiple antibiotic resistance due to a single mutation in Neisseria gonorrhoeae. J Infect Dis. 1973 Sep;128(3):321–330. doi: 10.1093/infdis/128.3.321. [DOI] [PubMed] [Google Scholar]
  13. Mayer L. W., Holmes K. K., Falkow S. Characterization of plasmid deoxyribonucleic acid from Neisseria gonorrhoeae. Infect Immun. 1974 Oct;10(4):712–717. doi: 10.1128/iai.10.4.712-717.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sanderson K. E. Linkage map of Salmonella typhimurium, edition IV. Bacteriol Rev. 1972 Dec;36(4):558–586. doi: 10.1128/br.36.4.558-586.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sarubbi F. A., Jr, Blackman E., Sparling P. F. Genetic mapping of linked antibiotic resistance loci in Neisseria gonorrhoeae. J Bacteriol. 1974 Dec;120(3):1284–1292. doi: 10.1128/jb.120.3.1284-1292.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Schoolnik G. K., Buchanan T. M., Holmes K. K. Gonococci causing disseminated gonococcal infection are resistant to the bactericidal action of normal human sera. J Clin Invest. 1976 Nov;58(5):1163–1173. doi: 10.1172/JCI108569. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sparling P. F. Genetic transformation of Neisseria gonorrhoeae to streptomycin resistance. J Bacteriol. 1966 Nov;92(5):1364–1371. doi: 10.1128/jb.92.5.1364-1371.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wiesner P. J., Handsfield H. H., Holmes K. K. Low antibiotic resistance of gonococci causing disseminated infection. N Engl J Med. 1973 Jun 7;288(23):1221–1222. doi: 10.1056/NEJM197306072882308. [DOI] [PubMed] [Google Scholar]

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